Modifications of mice gut microflora following oral consumption ofLactobacillus acidophilus and Bifidobacterium bifidum probiotics

Abstract

Background/aim: Thirty male BALB/c mice were equally divided into three groups: control, L. acidophilus, and B. bifidum for the assessment of the probiotics' stability in the gut microflora.Materials and methods: First, the gut microflora of the mice was checked every 3 days (days 3, 6, 9, and 12) without probiotic consumption, and then the mice were daily given orally 1.5 g of probiotics in 30 cc of drinking water. The consumption of probiotics was then stopped for recovery and then the consumption continued for 5 months.Results: On day 9 after the consumption of the probiotics, L. acidophilus and B. bifidum were significantly increased from 4% to 83% and from 1% to 61%, respectively. L. acidophilus count showed no significant decrease at the end of 5 months compared to day 9 of probiotic consumption (74%), but B. bifidum count was dramatically decreased to 45% and 36% at the end of 1 and 5 months, respectively. Conclusion:Our results revealed that, unlike B. bifidum, the amount of L. acidophilus remained almost unchanged in the long term, indicating more stability of L. acidophilus than B. bifidum in the gut microflora.

A Potential Novel Probiotic Strain and its Comparative Antagonism Evaluation with a Multi-Strain Probiotic Combination along with an Innovative Approach for Quantifying the Viable Microbiota

“…The inhibition zone diameter against C. albicans ATCC 10231 was 14.5±0.36 mm (15.0±0.0 mm, and 14.50±0.71 mm), which was the lowest ZOI (Table 3). The result of this experiment suggested that the Numerous earlier researches had demonstrated how the three probiotics (Source-1) can coexist and thrive at the same time [35,36,37]. They are the normal flora of the human intestine, and can grow mutually as well as suppress pathogens in the intestine.…”

Pterostilbene and Probiotic Complex in Chemoprevention of Putative Precursor Lesions for Colorectal Cancer in an Experimental Model of Intestinal Carcinogenesis with 1,2-Dimethylhydrazine

“…It has excellent tolerance to gastric acidity and bile, optimal intestinal mucosa adherence, and pathogen inhibitory actions, and favors immunological responses [ 19 ]. L. acidophilus shows greater stability than B. bifidum in the intestinal microflora of mice in the long-term (5 months) [ 20 ]. PRO with lactobacillus and bifidobacterium has a long history of safety [ 21 ].…”