Modifications of membrane lipids in response to wounding of <i>Arabidopsis thaliana</i> leaves

Vu, Hieu; Roston, Rebecca; Shiva, Sunitha; Hur, Manhoi; Wurtele, Eve Syrkin; Wang, Xuemin; Shah, Jyoti; Welti, Ruth

doi:10.1080/15592324.2015.1056422

Cited by 22 publications

(26 citation statements)

References 38 publications

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“…As the cells chill and the membranes become partially damaged, leakage of small ions including protons and Mg 2+ could provide a convenient mechanism for rapidly activating the membrane protective machinery, beginning with the activation of SFR2 at the outer chloroplast envelope membrane. Consistent with this hypothesis, wounding by a crushing force was observed to cause SFR2 activity (Vu et al, 2014b(Vu et al, , 2015. Wounded tissue allows cytoplasmic mixing with acidic apoplastic fluid and, possibly through this simple mechanism, SFR2 activation.…”

Section: Discussionsupporting

confidence: 55%

Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING2

2016

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Low temperature is a seasonal abiotic stress that restricts native plant ranges and crop distributions. Two types of lowtemperature stress can be distinguished: chilling and freezing. Much work has been done on the mechanisms by which chilling is sensed, but relatively little is known about how plants sense freezing. Recently, Arabidopsis (Arabidopsis thaliana) SENSITIVE TO FREEZING2 (SFR2) was identified as a protein that responds in a nontranscriptional manner to freezing. Here, we investigate the cellular conditions that allow SFR2 activation. Using a combination of isolated organelle, whole-tissue, and whole-plant assays, we provide evidence that SFR2 is activated by changes in cytosolic pH and Mg 2+ . Manipulation of pH and Mg 2+ in coldacclimated plants is shown to cause changes similar to those of freezing. We conclude that pH and Mg 2+ are perceived as intracellular cues as part of the sensing mechanism for freezing conditions. This evidence provides a specific molecular mechanism to combat freezing.

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Section: Discussionsupporting

confidence: 55%

Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING2

2016

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“…In our experiments, Arabidopsides, which are known to be induced upon wounding in wild‐type Arabidopsis plants (Buseman et al ., ; Kourtchenko et al ., ; Vu et al ., ), showed increased abundances in non‐wounded fer mutants compared with the wild‐type; however, the effect of wounding in fer mutants is unknown. Therefore, it is essential to study the effect of wounding on the levels of Arabidopsides in both genotypes in order to explore the significance of Arabidopsides in the FERONIA pathway.…”

Section: Resultsmentioning

confidence: 99%

“…One study found that very low levels of Arabidopsides are present in unwounded wild‐type leaves, but that the levels of Arabidopsides A, B, and D increased 200‐ to 1000‐fold 15 min after wounding (Buseman et al ., ). Another study reported increases of ~100‐ or ~50‐fold 45 min after wounding for Arabidopside A and B, respectively (Vu et al ., ). Arabidopsides E and G, containing an additional OPDA attached to the galactose group, have also been reported to be induced by abiotic stress (Kourtchenko et al ., ; Nilsson et al ., ; Vu et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Arabidopsides are well known to be induced by wounding (Buseman et al ., ; Kourtchenko et al ., ; Vu et al ., , ), especially in Arabidopsis from which the name originates. One study found that very low levels of Arabidopsides are present in unwounded wild‐type leaves, but that the levels of Arabidopsides A, B, and D increased 200‐ to 1000‐fold 15 min after wounding (Buseman et al ., ).…”

Section: Discussionmentioning

confidence: 99%

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FERONIA mutation induces high levels of chloroplast‐localized Arabidopsides which are involved in root growth

et al. 2018

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The FERONIA (FER) signaling pathway is known to have diverse roles in Arabidopsis thaliana, such as growth, reproduction, and defense, but how this receptor kinase is involved in various biological processes is not well established. In this work, we applied multiple mass spectrometry techniques to identify metabolites involved in the FER signaling pathway and to understand their biological roles. A direct infusion Fourier transform ion cyclotron resonance (FT-ICR)-MS approach was used for initial screening of wild-type and feronia (fer) mutant plant extracts, and Arabidopsides were found to be significantly enriched in the mutant. As Arabidopsides are known to be induced by wounding, further experiments on wounded and nonwounded leaf samples were carried out to investigate these oxylipins as well as related phytohormones using a quadrupole-time-of-flight (Q-TOF) MS by direct injection and LC-MS/MS. In a root growth bioassay with Arabidopside A isolated from fer mutants, the wild-type showed significant root growth inhibition compared with the fer mutant. Our results therefore implicated Arabidopsides, and Arabidopside A specifically, in FER functions and/or signaling. Finally, matrix-assisted laser desorption/ionization MS imaging (MALDI-MSI) was used to visualize the localization of Arabidopsides, and we confirmed that Arabidopsides are highly abundant at wounding sites in both wild-type and fer mutant leaves. More significantly, five micron high-spatial resolution MALDI-MSI revealed that Arabidopsides are localized to the chloroplasts where many stress signaling molecules are made.

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“…Firstly, these molecules are largely produced under stress conditions. For example, A. thaliana esterified oxylipins peak under a wide range of different biotic and abiotic stresses, such as wounding, freezing and pathogen infection (Buseman et al 2006;Böttcher and Weiler 2007;Kourtchenko et al 2007;Glauser et al 2008Glauser et al , 2009Chechetkin et al 2009Chechetkin et al , 2013Koo et al 2009;Vu et al 2012Vu et al , 2015. Moreover, upon wounding, arabidopsides not only accumulate in wounded leaves but also in distal unwounded leaves of the same plant (Glauser et al 2009).…”

Section: Esterified Oxylipin Involvement In Plant Defense and Developmentioning

confidence: 99%

New insights into the biosynthesis of esterified oxylipins and their involvement in plant defense and developmental mechanisms

et al. 2018

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Plant oxylipins produced following oxidation of unsaturated fatty acids are structurally diverse metabolites that play crucial developmental and defensive roles. Whereas free oxylipins are well studied, oxylipins esterified in complex lipids such as galacto-and phospholipids are thought to be rare and have unclear roles. In the last few years, new analytical methods have been developed, leading to the discovery of many esterified oxylipins in a variety of plant species. This suggests that these molecules may be ubiquitous plant metabolites. While their precise functions are unclear, esterified oxylipins seem to play important roles in plant development and defense. This review focuses on new insights regarding diversity, biosynthesis and function of those interesting and understudied molecules. Keywords Arabidopsides Á Jasmonates Á Lipoxygenase Á Oxylipins Á Plant defense Abbreviations (dn)OPDA OPDA and dnOPDA A. thaliana Arabidopsis thaliana (L.) Heynh ACX1 Acyl-coenzyme A oxidase 1 ADH Alcohol dehydrogenase AGAP1 Acylated galactolipid associated phospholipase 1 AOC Allene oxide cyclase AOS Allene oxide synthase DES Divinyl ether synthase 4,5-ddhJA 4,5-Didehydro-jasmonic acid DGDG Digalactosyldiacylglycerol DGMG Digalactosylmonoacylglycerol dnOPDA 12-Dinor-oxo-phytodienoic acid DOX a-Dioxygenase DW Dry weight EAS Epoxy alcohol synthase EPB Epibrassinolide FW Fresh weight GC Gas chromatography

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Modifications of membrane lipids in response to wounding of Arabidopsis thaliana leaves

Cited by 22 publications

References 38 publications

Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING2

Chloroplast Membrane Remodeling during Freezing Stress Is Accompanied by Cytoplasmic Acidification Activating SENSITIVE TO FREEZING2

FERONIA mutation induces high levels of chloroplast‐localized Arabidopsides which are involved in root growth

New insights into the biosynthesis of esterified oxylipins and their involvement in plant defense and developmental mechanisms

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