Modifications induced by diabetes on the physicochemical and functional properties of erythrocyte plasma membrane

Mazzanti, Laura; Rabini, R. A.; Testa, I; Bertoli, Enrico

doi:10.1111/j.1365-2362.1989.tb00311.x

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…These results suggest two hypotheses: i ) that acute hyperglycemia exerts different effects on the plasma membrane in comparison with chronic hyperglycemia or 2) that the alterations observed in type 1 diabetic platelet membranes are not caused by the effects of glucose but by the disease per se, which might act through different pathogenetic mechanisms, such as modifications in the lipid structure of the membrane, hi both the present and previous works by our group (32,33), no relation was observed between cellular membrane fluidity and/or enzymatic activities and HbA lc and/or fasting glycemia in diabetic patients, which seems to give support to the latter hypothesis, i.e., that diabetes might affect plasma membranes in a way not strictly dependent on plasma glucose levels.…”

Section: 2mentioning

confidence: 58%

Altered Platelet Membrane Dynamic Properties in Type 1 Diabetes

et al. 1997

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A modified platelet response to aggregating stimuli is supposed to play a role in the pathogenesis of diabetic macroangiopathy. We studied the fluidity and microheterogeneity of the external surface of the platelet membrane and the activities of the plasma membrane Na+-K+-ATPase and Ca2+-ATPase in 21 men with type 1 diabetes and in 20 control subjects before and after in vitro thrombin addition. In the resting state, platelets from type 1 diabetic patients showed an increased fluidity and microheterogeneity of the platelet membrane, a higher Ca2+-ATPase activity, and a reduced Na+-K+-ATPase activity in comparison with platelets from healthy subjects. The fatty acid composition was also modified, with increased C 16:1 and decreased C 18:0 content. Control cells incubated with thrombin showed a modification of the membrane parameters opposite to the response observed in type 1 cells after the stimulation. The incubation of control platelets in the resting state with high concentrations of glucose modified the fluidity of the plasma membrane Na+-K+-ATPase and Ca2+-ATPase activities in an opposite way in comparison with the alterations observed in type 1 platelets. This study suggests that in type 1 diabetic patients, the platelet membrane responds to activation with a molecular remodeling different from the response of healthy subjects. The abnormal organization of the membrane might contribute to the altered platelet functions in type 1 diabetic patients, but acute exposure to high glucose levels does not seem able to modify the platelet membrane in the way observed in type 1 diabetes.

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Section: 2mentioning

confidence: 58%

Altered Platelet Membrane Dynamic Properties in Type 1 Diabetes

et al. 1997

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“…The basal fluidity of erythrocyte membranes from healthy subjects and patients with non-insulindependent diabetes mellitus was shown to be different. Increase [15,16], decrase [17,18] or no change [19][20][21] in the membrane fluidity of erythrocytes of patients with non-insulin-dependent diabetes mellitus have been reported in the literature. The treatment of patients and the analysis techniques used could be responsible for the controversial results obtained.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the alterations induced in erythrocyte membrane physical characteristics of non-insulin-dependent type II diabetic Patients: an ESR study

Genç

Korkmaz

2006

Appl. Magn. Reson.

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The physical and dynamic properties of intact erythrocyte membranes of two groups, diabetic patients treated by diet alone (27 women and 41 men, 40-75 years old) and healthy subjects (controls) (25 women and 35 men, 35-75 years old) were investigated by electron spin resonance (ESR) spectroscopy. 5-doxyl stearic acid (5-DSA) and 4-maleimido-2,2,6,6-tetramethyl-piperidine-1-oxyl (MAL-6) are employed as spin labels throughout the experiments. While 5-DSA monitors the membrane surface region and gives information about the order of lipids in this region through the order parameters S', MAL-6 binds thiol groups S-H to the membrane protein and gives information about possible protein conformational changes. Order parameters were calculated from room temperature spectra recorded for intact erythrocytes labeled with 5-DSA and the values of 0.682__.0.004 and 0.666+0.004 were obtained for diabetic patients and controls, respectively. Variations of the ht_~), h~o v ht+t) and 2A, parameters in the temperature range of 10-50 ~ were also studied and fairly different behaviors were observed for both groups, especially for the ht_~) parameter. Line intensities W and S characte¡ the concentrations of weakly and strongly immobilized MAL-6 species bound to S-H groups of intact erythrocyte membrane proteins were used to calculate the W/S ratios. They were found to be 34.1+_ 1.9 and 30.5-+2.4 for diabetic patients and controls, respectively. While line intensities T, those of the M~ = -I high-field resonance line associated with the weakly immobilized S-H sites, of both groups exhibit a very similar microwave saturation behavior in the range of 0.5-20 mW, line intensities W of both groups saturated differently. Va¡ of line intensities S, W and T with temperature were also investigated in the temperature range of 10-50 ~ and characteristic differences were observed to exist between two groups implying a less fluid erythrocyte membrane for diabetic patients. Increases in the order parameter S' and in the W/S ratio are considered as major factors attributable to the alteration in the physical and dynamic properties of erythrocyte membrane in the diabetic state.

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