Modification of titanium implants using biofunctional nanodiamonds for enhanced antimicrobial properties

Krok, Emilia; Balakin, Sascha; Jung, Jonas; Groß, Frank; Opitz, Joerg; Cuniberti, Gianaurelio

doi:10.1088/1361-6528/ab6d9b

Cited by 9 publications

(4 citation statements)

References 50 publications

(66 reference statements)

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“…This distinct characteristic, compared to other surgical infections, requires a prolonged antimicrobial activity [8,14,15]. Direct conjugation of antimicrobial molecules [16], deposition of drug-loaded nanoporous titanium layers [17], nanodiamonds [18] or single drug eluting coatings [19,20] on the surface of titanium are examples of the numerous approaches have been attempted to prepare antimicrobial releasing titanium surfaces; these approaches however have not demonstrated antimicrobial activity for prolong period of time as drug release was completed within hours or days, therefore their performance is still not fully satisfactory. The most promising releasing system, in terms of being able to sustain drug elution for extended periods of time, is multilayers coatings; these consist of oppositely charged polyelectrolytes alternately deposited on the surface of implants [21]; during the deposition, the drug is entrapped between layers and is later released via film-erosion or diffusion [22].…”

Section: Introductionmentioning

confidence: 99%

Long acting anti-infection constructs on titanium

Perni

Alotaibi

Yergeshov

et al. 2020

Journal of Controlled Release

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Peri-prosthetic joint infections (PJI) are a serious adverse event following joint replacement surgeries; antibiotics are usually added to bone cement to prevent infection offset. For uncemented prosthesis, alternative antimicrobial approaches are necessary in order to prevent PJI; however, despite elution of drug from the surface of the device being shown one of the most promising approach, no effective antimicrobial eluting uncemented device is currently available on the market. Consequently, there is a clinical need for non-antibiotic antimicrobial uncemented prosthesis as these devices present numerous benefits, particularly for young patients, over cemented artificial joints. Moreover, non-antibiotic approaches are driven by the need to address the growing threat posed by antibiotic resistance.We developed a multilayers functional coating on titanium surfaces releasing chlorhexidine, a wellknown antimicrobial agent used in mouthwash products and antiseptic creams, embedding the drug between alginate and poly-beta-amino-esters.Chlorhexidine release was sustained for almost 2 months and the material efficacy and safety was proven both in vitro and in vivo. The coatings did not negatively impact osteoblast and fibroblast cells growth and were capable of reducing bacterial load and accelerating wound healing in an excisional wound model.As PJI can develop weeks and months after the initial surgery, these materials could provide a viable solution to prevent infections after arthroplasty in uncemented prosthetic devices and, simultaneously, help the fight against antibiotic resistance.

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Section: Introductionmentioning

confidence: 99%

Long acting anti-infection constructs on titanium

Perni

Alotaibi

Yergeshov

et al. 2020

Journal of Controlled Release

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“…The secretion of these bacteria on implant surfaces forms biofilms, which protect them against the immune system and antibacterial agents. Consequently, treatment with antibiotics has become a vital issue 19 . Since bacteria and osteoblast cells are in competition for attachment, implants having antibacterial surface characteristics can decrease bacterial attachment and colony formation so they can be used for treating bone defects 20 , 21 .…”

Section: Introductionmentioning

confidence: 99%

Effect of electrochemical oxidation and drug loading on the antibacterial properties and cell biocompatibility of titanium substrates

Nowruzi

Imani

Faghihi

2022

Sci Rep

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A combination of $${\text{ TiO}}_{2}$$ TiO 2 nanotube array (TON) and controlled drug release system is employed to provide enhanced surface properties of titanium implants. Electrochemical anodization process is used to generate TON for introducing, vancomycin, an effective antibacterial drug against Staphylococcusaureus. TON loaded vancomycin is then coated with a number of layers of 10% gelatin using spin coating technique. The gelatin film is reinforced with graphene oxide (GO) nanoparticles to improve the surface bioactivity. The surface of the samples is characterized by field emission electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDS), and contact angle measurement. The results illustrate that the TON was constructed and vancomycin molecules are successfully loaded. The drug release study shows that the amount of released vancomycin is controlled by the thickness of gelatin layers. With an increase in gelatin film layers from 3 to 7, the release of vancomycin in the burst release phase decreased from 58 to 31%, and sustained release extended from 10 to 17 days. The addition of GO nanoparticles seems to reduce drug release in from 31 to 22% (burst release phase) and prolonged drug release (from 17 to 19 days). MTT assay indicates that samples show no cytotoxicity, and combination of GO nanoparticles with gelatin coating could highly promote MG63 cell proliferation. Soaking the samples in SBF solution after 3 and 7 days demonstrates that hydroxy apatite crystals were deposited on the TON surface with GO-gelatin coating more than surface of TON with gelatin. Moreover, based on the results of disc diffusion assay, both samples (loaded with Vancomycin and coated with gelatin and gelatin-GO) with the inhibition zones equaled to 20 mm show effective antibacterial properties against S. aureus. The evidence demonstrates that titania nanotube loaded with vancomycin and coated with gelatin-GO has a great potential for general applicability to the orthopedic implant field.

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“…It may prevent the occurrence of microbial growth, increase protection against ions liberation, stimulate cellular growth, and increase mechanical features, among many other improvements. Research projects assess how the different combined modifications can foment better healing mechanisms to patients [35][36][37] .…”

Section: Introductionmentioning

confidence: 99%

Titanium-Tantalum Alloy Surface Modification by Hydroxyapatite Layer on TiO2 Nanotubes: Effect on Microbial Activity

et al. 2021

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One of the major health security challenges of the 21st century is the occurrence of microbial infections and bacterial complications that could affect 10 million people by 2050. On the biomaterial field, implant metallic currently replaces partial or total body parts and can fail to be integrated into the body due to infections. This study performs two combined surface modifications on Ti-30Ta alloy, in order to obtain an infection-resistance and osseointegration surface on metallic implants to be tested within bacterial biofilm. The Group 1 investigated surface modifications by the anodization process in the electrolyte glycerol + NH 4 F 0.25% at 30V-9 hours and annealed in 530°C (5°C/min). The Group 2 underwent the same process as Group 1 and, additionally, the samples were immersed in 0.3 M CaCl 2 and 0.5 M Na 2 HPO 4 solutions for hydroxyapatite growth. The substrate was characterized using scanning electron microscopy (SEM), X-ray diffractometer (XRD) and dynamic contact angle. S. epidermidis bacterial adhesion and biofilm formation. The results indicated that the Group 1 shows a higher antimicrobial activity, hydrophilic behavior and potential to be used for metallic implant applications. The Group 2 with the hydroxyapatite film coating did not have an improvement in the antimicrobial response.

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Modification of titanium implants using biofunctional nanodiamonds for enhanced antimicrobial properties

Cited by 9 publications

References 50 publications

Long acting anti-infection constructs on titanium

Long acting anti-infection constructs on titanium

Effect of electrochemical oxidation and drug loading on the antibacterial properties and cell biocompatibility of titanium substrates

Titanium-Tantalum Alloy Surface Modification by Hydroxyapatite Layer on TiO2 Nanotubes: Effect on Microbial Activity

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