Modification of the Glucosamine Method for the Quantification of Fungal Contamination

Mills, Paul A; Rotter, R. G.; Marquardt, R. R.

doi:10.4141/cjas89-128

Cited by 50 publications

(24 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nitrogen was determined using an N analyzer (Model CNS-2000, Leco Corporation, St. Joseph, MI). Samples for AA analysis were prepared by acid hydrolysis according to the method of AOAC (1984; method 982.30) as modified by Mills et al (Mills et al 1989). Briefly, approximately 100 mg of each sample was digested in 4 mL of 6 N HCl for 24 h at 110 °C, followed by neutralization with 4 mL of 25 % (wt/vol) NaOH and further cooled to room temperature.…”

Section: Analysis Of Conventional Indexmentioning

confidence: 99%

Low-protein diets affect ileal amino acid digestibility and gene expression of digestive enzymes in growing and finishing pigs

et al. 2015

Amino Acids

View full text Add to dashboard Cite

The objective of this study was to evaluate effects of dietary crude protein (CP) intake on ileal amino acid digestibilities and expression of genes for digestive enzymes in growing and finishing pigs. In Experiment 1, 18 growing pigs (average initial BW = 36.5 kg) were assigned randomly into one of three treatments (n = 6/treatment group) representing normal (18 % CP), low (15 % CP), and very low (12 % CP) protein intake. In Experiment 2, 18 finishing pigs (average initial BW = 62.3 kg) were allotted randomly into one of three treatments (n = 6/treatment group), representing normal (16 % CP), low (13 % CP) and very low (10 % CP) protein intake. In both experiments, diets with low and very low CP were supplemented with crystalline amino acids to achieve equal content of standardized ileal digestible Lys, Met, Thr, and Trp, and were provided to pigs ad libitum. Daily feed intake, BW, and feed/gain ratios were determined. At the end of each experiment, all pigs were slaughtered to collect pancreas, small-intestine samples, and terminal ileal chymes. Samples were used for determining expression of genes for digestive enzymes and ileal amino acid digestibilities. Growing pigs fed the 12 % CP and 15 % CP diets had lower final body weight (P < 0.01) and ADG (P < 0.0001) when compared with pigs fed the 18 % dietary CP diet. Growing pigs fed with the 12 % CP diet showed higher digestibilities for CP (P < 0.05), DM (P < 0.05), Lys (P < 0.0001), Met (P < 0.01), Cys (P < 0.01), Thr (P < 0.01), Trp (P < 0.05), Val (P < 0.05), Phe (P < 0.05), Ala (P < 0.05), Cys (P < 0.01), and Gly (P < 0.05) than those fed the 18 % CP diet. Finishing pigs fed the 16 % CP diet had a higher (P < 0.01) final body weight than those fed the 10 % CP diet. mRNA levels for digestive enzymes (trypsinogen, chymotrypsin B, and dipeptidases-II and III) differed among the three groups of pigs (P < 0.05), and no difference was noted in the genes expression between control group and lower CP group. These results indicated that a reduction of dietary CP by a six-percentage value limited the growth performance of growing-finishing pigs and that a low-protein diet supplemented with deficient amino acids could reduce the excretion of nitrogen into the environment without affecting weight gain.

show abstract

Section: Analysis Of Conventional Indexmentioning

confidence: 99%

Low-protein diets affect ileal amino acid digestibility and gene expression of digestive enzymes in growing and finishing pigs

et al. 2015

Amino Acids

View full text Add to dashboard Cite

show abstract

“…Titanium dioxide content in the diet and ileal samples was measured according to the method described by Lomer et al (2000). The AA content was determined using a LKB 4151 Alpha plus AA analyzer (LKB Biochrom, Cambridge, UK) according to AOAC procedures (1990; method 944.12), and as modified by Mills et al (1989) as described by Woyengo et al (2010a). Briefly, about 100 mg of each sample was hydrolyzed with 6 N HCl, sealed, evacuated, and digested at 110 • C for 24 h. Before hydrolysis, cysteine and methionine were oxidized with formic acid.…”

Section: Sample Preparation and Chemical Analysesmentioning

confidence: 99%

Ileal amino acid digestibility in egg from hyperimmunized-hens fed to weaned pigs and piglet response to diets contain egg products

Heo

Woyengo

Kahindi

et al. 2015

Animal Feed Science and Technology

View full text Add to dashboard Cite

“…The SECM and EECM samples were analyzed for NDF according to the method of Van Soest et al (1991) using an Ankom 200 Fiber Analyzer (Ankom Technology, Fairport, NY) and for EE using hexane as the solvent according to the AOAC (1990; method 920.39). Samples for AA analysis were prepared by acid hydrolysis according to the method of AOAC (1990; method 982.30) and as modified by Mills et al (1989). Briefly, about 100 mg of each sample was digested in 4 mL of 6 N HCl for 24 h at 110°C, followed by neutralization with 4 mL of 25% (wt/vol) NaOH, and was cooled to room temperature.…”

Section: Sample Preparation and Analysesmentioning

confidence: 99%

Metabolizable energy and standardized ileal digestible amino acid contents of expeller-extracted canola meal fed to broiler chicks

2010

View full text Add to dashboard Cite

An experiment was conducted to determine the standardized ileal digestible amino acid (AA) and AMEn contents of expeller-extracted canola meal (EECM) fed to broiler chicks. One hundred forty-four broiler chicks were divided into 24 groups of 6 birds balanced for BW and fed 4 diets in a completely randomized design (6 groups per diet) from d 14 to 21 of age. The diets were a corn-soybean meal-based basal diet formulated to meet NRC nutrient requirements, the basal diet with energy- and AA-yielding ingredients replaced by 30% of either solvent-extracted canola meal (SECM) or EECM, and a low-protein casein-cornstarch-based diet. The SECM, which is commonly used in formulating poultry diets, was fed for comparison with EECM, whereas the casein-cornstarch-based diet was fed to estimate basal endogenous AA losses for determining standardized ileal digestibility of AA. All 4 diets contained titanium oxide (0.3%) as an indigestible marker, and nutrient digestibility and retention were determined by the substitution method. From d 19 to 21, excreta samples were collected for AMEn determination. On d 21, the birds were killed by cervical dislocation, and contents of the whole ileum were obtained for determination of AA digestibility. Expeller-extracted canola meal had greater (P<0.05) standardized ileal digestible contents of Gly, Leu, Ser, Thr, Asp, and Glu and tended to have greater (P<0.10) standardized ileal digestible contents of Ile, Lys, Phe, Ala, and Tyr than SECM. Compared with SECM, EECM had greater (P<0.001) AMEn (2,694 vs. 1,801 kcal/kg). The results show that the EECM evaluated in the current study had greater standardized ileal digestible AA and AMEn contents than SECM, and hence, it may be a better source of protein and energy for broiler chicks than SECM. The standardized ileal digestible AA and AMEn values of EECM used in the current study could be used when formulating broiler chicken diets using the same to minimize the N excretion and feeding cost.

show abstract

Modification of the Glucosamine Method for the Quantification of Fungal Contamination

Cited by 50 publications

References 1 publication

Low-protein diets affect ileal amino acid digestibility and gene expression of digestive enzymes in growing and finishing pigs

Low-protein diets affect ileal amino acid digestibility and gene expression of digestive enzymes in growing and finishing pigs

Ileal amino acid digestibility in egg from hyperimmunized-hens fed to weaned pigs and piglet response to diets contain egg products

Metabolizable energy and standardized ileal digestible amino acid contents of expeller-extracted canola meal fed to broiler chicks

Contact Info

Product

Resources

About