Modification of the Colorimetric Assay for Serum Unsaturated Iron-binding Capacity

Yamanishi, Hiroshi; Iyama, Shigeru; Yamaguchi, Yoshihiro; Kanakura, Yuzuru; Iwatani, Yoshinori

doi:10.1373/49.6.1023

Cited by 7 publications

(7 citation statements)

References 3 publications

(2 reference statements)

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“…The negative proportional bias was ~8%, meaning that results gained by the Olympus »calculation method« were 91.9% of the results obtained by the reference method. These findings are similar with those reported in other studies (9,(12)(13)(14).…”

Section: Discussionsupporting

confidence: 93%

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Reference interval for calculated total iron-binding capacity using Olympus AU2700 analyzer

Jovicic

Ignjatović

Dajak

et al. 2005

Jugoslav Med Biohem

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Total iron-binding capacity (TIBC) values are determined on Olympus AU2700 automated chemistry analyzer as the sum of serum iron and unsaturated iron-binding capacity (UIBC)-(calculated TIBC, TIBC cal). Considering that Olympus AU2700 automated analyzer was recently brought in function and TIBC values calculated from serum iron and UIBC values were significantly lower than those obtained by a direct and fully automated TIBC assay, it was necessary to determine the reference interval for TIBC, according to the recommendation that every laboratory should have its own reference ranges. The »calculation method« of TIBC determination showed satisfactory accuracy (p > 0.001) and precision, with CV values ranging from 0.91% to 1.63% within-run and from 2.30% to 2.80% day-today. The correlation between the TIBC values obtained with the »calculation method« using Olympus AU2700 analyzer (y) and those obtained with the direct method (x) was: y = 0.919x + 2.319 mmol/L (r = 0.980; Sy,x = 1.814; p < 0.001; N = 85). The reference interval for TIBC was determined using sera collected from 125 healthy individuals of both sexes, 15 to 80 years old. Since the values did not depend on sex, the reference interval calculated for the whole studied population ranged between 42.0 mmol/L and 64.3 mmol/L.

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Section: Discussionsupporting

confidence: 93%

“…As the number of unsaturated sites increases, more time is required for the reaction to come to completion. Therefore, the low UIBC result obtained by the automated UIBC method is attributable to insufficient time allowed for the saturation of transferrin (9,14).…”

Section: Discussionmentioning

confidence: 99%

Reference interval for calculated total iron-binding capacity using Olympus AU2700 analyzer

Jovicic

Ignjatović

Dajak

et al. 2005

Jugoslav Med Biohem

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“…Most iron in plasma is bound to transferrin and iron is loaded into transferrin principally through the activity of the ferroxidase, ceruloplasmin. 261 Ceruloplasmin is expressed in glia in the brain 262 as well as being abundant in plasma. The specific ferroxidase activity of ceruloplasmin is decreased B80% in the substantia nigra of idiopathic Parkinson's disease cases, possibly related to a marked drop in copper levels in the tissue.…”

Section: The Breakdown In Metal Homeostasis In Parkinson's Diseasementioning

confidence: 99%

Biological metals and metal-targeting compounds in major neurodegenerative diseases

2014

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Multiple abnormalities occur in the homeostasis of essential endogenous brain biometals in age-related neurodegenerative disorders, Alzheimer's disease, Parkinson's disease, Huntington's disease and amyotrophic lateral sclerosis. As a result, metals both accumulate in microscopic proteinopathies, and can be deficient in cells or cellular compartments. Therefore, bulk measurement of metal content in brain tissue samples reveal only the "tip of the iceberg", with most of the important changes occurring on a microscopic and biochemical level. Each of the major proteins implicated in these disorders interacts with biological transition metals. Tau and the amyloid protein precursor have important roles in normal neuronal iron homeostasis. Changes in metal distribution, cellular deficiencies, or sequestration in proteinopathies all present abnormalities that can be corrected in animal models by small molecules. These biochemical targets are more complex than the simple excess of metals that are targeted by chelators. In this review we illustrate some of the richness in the science that has developed in the study of metals in neurodegeneration, and explore its novel pharmacology.

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“…Iron-binding capacity (IBC) was calculated from serum iron and transferrin. 26 Plasma zinc and selenium were assayed by atomic absorption spectrometry by the Nutrition Centre of the South African Medical Research Council. The children's HIV status was assessed by enzyme-linked immunosorbent assay and positive results confirmed by a second assay, or in children younger than 18 months, by polymerase chain reaction.…”

Section: Micronutrient Investigationsmentioning

confidence: 99%

Nutritional status and its response to treatment of children, with and without HIV infection, hospitalized for the management of tuberculosis

Schaaf

Cilliers

Willemse

et al. 2012

Paediatrics and International Child Health

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Modification of the Colorimetric Assay for Serum Unsaturated Iron-binding Capacity

Cited by 7 publications

References 3 publications

Reference interval for calculated total iron-binding capacity using Olympus AU2700 analyzer

Reference interval for calculated total iron-binding capacity using Olympus AU2700 analyzer

Biological metals and metal-targeting compounds in major neurodegenerative diseases

Nutritional status and its response to treatment of children, with and without HIV infection, hospitalized for the management of tuberculosis

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