Modification of the Cadmium Reduction Assay for Detection of Nitrite Production Using Fluorescence Indicator 2,3-Diaminonaphthalene

Casey, Terrie E.; Hilderman, Richard H.

doi:10.1006/niox.1999.0265

Cited by 12 publications

(7 citation statements)

References 14 publications

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“…The assay is sensitive, able to measure concentrations of nitrite as low as ~0.5 mM and is available in various commercial kits. NO x can also be determined with fluorescent [82] and chemiluminescence [83] assays. However, its use in a clinical setting is questioned, mainly due to the fact that dietary intake of nitrate (mostly derived from proteins) heavily influences the values measured in human biological samples [84].…”

Section: Mda/tbarsmentioning

confidence: 99%

Evaluating Oxidative Stress in Human Cardiovascular Disease: Methodological Aspects and Considerations

Lee¹,

Margaritis²,

Channon³

et al. 2012

CMC

214

162

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Oxidative stress is a key feature in atherogenesis, since reactive oxygen species (ROS) are involved in all stages of the disease, from endothelial dysfunction to atheromatic plaque formation and rupture. It is therefore important to identify reliable biomarkers allowing us to monitor vascular oxidative stress status. These may lead to improved understanding of disease pathogenesis and development of new therapeutic strategies.Measurement of circulating biomarkers of oxidative stress is challenging, since circulation usually behaves as a separate compartment to the individual structures of the vascular wall. However, measurement of stable products released by the reaction of ROS and vascular/circulating molecular structures is a particularly popular approach. Serum lipid hydroperoxides, plasma malondialdehyde or urine F2-isoprostanes are widely used and have a prognostic value in cardiovascular disease.Quantification of oxidative stress at a tissue level is much more accurate. Various chemiluminescence and high performance liquid chromatography assays have been developed over the last few years, and some of them are extremely accurate and specific. Electron spin resonance spectroscopy and micro-electrode assays able to detect ROS directly are also widely used.In conclusion, measurement of circulating biomarkers of oxidative stress is valuable, and some of them appear to have predictive value in cardiovascular disease. However, these biomarkers do not necessarily reflect intravascular oxidative stress and therefore cannot be used as therapeutic targets or markers to monitor pharmacological treatments in clinical settings. Measurement of vascular oxidative stress status is still the only reliable way to evaluate the involvement of oxidative stress in atherogenesis.

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Section: Mda/tbarsmentioning

confidence: 99%

Evaluating Oxidative Stress in Human Cardiovascular Disease: Methodological Aspects and Considerations

Lee¹,

Margaritis²,

Channon³

et al. 2012

CMC

214

162

View full text Add to dashboard Cite

show abstract

“…Due to expense, time-consuming procedures and/or limitations in sensitivity of these methods, total NOx is typically determined by converting nitrate into nitrite followed by measuring total amount of nitrite in the sample. In this widely used practice, nitrite concentration is typically measured by a number of additional well-known methods such as colorimetric Griess assay [15,17], fluorescent assays [13,18], chemiluminescence assay [19] and electrochemical detection [11]. To determine nitrate concentration specifically, the same sample is measured twice separately with and without a reducing agent.…”

Section: Introductionmentioning

confidence: 99%

Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay

Sun

Zhang

Broderick

et al. 2003

Sensors

575

301

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“…Griess reaction was first described in 1879 . Because of its simplicity, it has been used extensively in the analysis of numerous natural or biological samples including plasma, urine, or saliva . In this method, nitrite is first treated in acidic media with a diazotizing reagent (sulfanilamide, SA) to form a transient diazonium salt.…”

mentioning

confidence: 99%

Boosting the Quantitative Inorganic Surface-Enhanced Raman Scattering Sensing to the Limit: The Case of Nitrite/Nitrate Detection

Correa‐Duarte

Pazos-Perez

Guerrini

et al. 2015

J. Phys. Chem. Lett.

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A high-performance ionic-sensing platform has been developed by an interdisciplinary approach, combining the classical colorimetric Griess reaction and new concepts of nanotechnology, such as plasmonic coupling of nanoparticles and surface-enhanced Raman scattering (SERS) spectroscopy. This approach exploits the advantages of combined SERS/surface-enhanced resonant Raman Scattering (SERRS) by inducing the formation of homogeneous hot spots and a colored complex in resonance with the laser line, to yield detection limits for nitrite down to the subpicomolar level. The performance of this new method was compared with the classical Griess reaction and ionic chromatography showing detection limits about 6 and 3 orders of magnitude lower, respectively.

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Modification of the Cadmium Reduction Assay for Detection of Nitrite Production Using Fluorescence Indicator 2,3-Diaminonaphthalene

Cited by 12 publications

References 14 publications

Evaluating Oxidative Stress in Human Cardiovascular Disease: Methodological Aspects and Considerations

Evaluating Oxidative Stress in Human Cardiovascular Disease: Methodological Aspects and Considerations

Measurement of Nitric Oxide Production in Biological Systems by Using Griess Reaction Assay

Boosting the Quantitative Inorganic Surface-Enhanced Raman Scattering Sensing to the Limit: The Case of Nitrite/Nitrate Detection

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