Modification of stimulated lacrimal gland flow by sympathetic nerve impulses in rabbit

Botelho, SY; Martinez, EV; Pholpramool, Chumpol; Prooyen, HC; Janssen, JT; Palau, Antoni

doi:10.1152/ajplegacy.1976.230.1.80

Cited by 56 publications

(21 citation statements)

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“…Bolus injections of ACh were used to allow comparison of the present results with those of previous studies on rabbit in vivo lacrimal gland secretion, all of which employed bolus stimulation (Botelho, Goldstein & Hisada, 1969;Kikkawa, 1970;Botelho & Martinez, 1973;Botelho, Martinez, Pholpramool, van Prooyen, Janssen & De Palau, 1976;Horowitz, Lammers, Sussman & Botelho, 1976;Pholpramool & Tangkrisanavinont, 1976;Botelho et al 1978;Tangkrisanavinont & Pholpramool, 1979;Botelho & Dartt, 1980). Furthermore, bolus injections were used because ACh injected into the lacrimal gland arterial supply enters the systemic circulation through the venous drainage of the lacrimal gland and causes a decrease in femoral artery mean and diastolic pressure (Dartt, 1978) To determine the effect of unlabelled ouabain concentration on [3H]ouabain binding, slices were preincubated as already described and then incubated for 60 min in KHB containing 0 5 x 10-7 M-[3H]ouabain either alone or with unlabelled ouabain to give a final concentration of 10-4 M. The extracellular space marker was added and the slices washed and prepared for scintillation counting as already described.…”

Section: Methodsmentioning

confidence: 93%

Lacrimal gland electrolyte and water secretion in the rabbit: localization and role of (Na+ + K+)‐activated ATPase.

Dartt¹,

Møller²,

Poulsen³

1981

The Journal of Physiology

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SUMMARY1. The rate of acetylcholine (ACh)-induced fluid secretion was measured from the main excretory duct of rabbit lacrimal glands perfused in vivo with Krebs Henseleit bicarbonate solutions.2. Perfusion with ouabain (10-5 M) decreased the rate of lacrimal gland fluid secretion to 23 % of the control value.3. Perfusion with furosemide (10-4 and 1O-3 M), which has been shown to inhibit the coupled transport of Na+ and Cl-, reversibly decreased the rate of secretion to 43 and 33 % of the control value respectively. 6. It is concluded that ACh-induced secretion of electrolytes and water is dependent upon (Na+ + K+)-activated ATPase. In addition, coupled transport of Na+ and Cl-appears to be involved in secretion.7. Basolateral location of the (Na+ + K+)-activated ATPase implies that it plays an indirect role in electrolyte and water secretion. A possible role may be to energize a secondary active transport of Cl-that is mediated by a NaCl cotransport system.

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Section: Methodsmentioning

confidence: 93%

Lacrimal gland electrolyte and water secretion in the rabbit: localization and role of (Na+ + K+)‐activated ATPase.

Dartt¹,

Møller²,

Poulsen³

1981

The Journal of Physiology

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“…First they can alter blood flow, with vasodilation increasing electrolyte and water secretion, and with vasoconstriction decreasing them [265]. Second, sympathetic neurotransmitters can directly induce protein, electrolyte and water secretion [243,261,266,267].…”

Section: Neural Regulation Of Tear Productionmentioning

confidence: 99%

TFOS DEWS II pain and sensation report

et al. 2017

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Pain associated to mechanical and chemical irritation of the eye surface is mediated by trigeminal ganglia mechano- and polymodal nociceptor neurons while cold thermoreceptors detect wetness and reflexly maintain basal tear production and blinking rate. These neurons project into two regions of the trigeminal brain stem nuclear complex: ViVc, activated by changes in the moisture of the ocular surface and VcC1, mediating sensory-discriminative aspects of ocular pain and reflex blinking. ViVc ocular neurons project to brain regions that control lacrimation and spontaneous blinking and to the sensory thalamus. Secretion of the main lacrimal gland is regulated dominantly by autonomic parasympathetic nerves, reflexly activated by eye surface sensory nerves. These also evoke goblet cell secretion through unidentified efferent fibers. Neural pathways involved in the regulation of Meibonian gland secretion or mucins release have not been identified. In dry eye disease, reduced tear secretion leads to inflammation and peripheral nerve damage. Inflammation causes sensitization of polymodal and mechano-nociceptor nerve endings and an abnormal increase in cold thermoreceptor activity, altogether evoking dryness sensations and pain. Long-term inflammation and nerve injury alter gene expression of ion channels and receptors at terminals and cell bodies of trigeminal ganglion and brainstem neurons, changing their excitability, connectivity and impulse firing. Perpetuation of molecular, structural and functional disturbances in ocular sensory pathways ultimately leads to dysestesias and neuropathic pain referred to the eye surface. Pain can be assessed with a variety of questionaires while the status of corneal nerves is evaluated with esthesiometry and with in vivo confocal microscopy.

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“…Moreover, α1-adrenergic receptors exist in lacrimal gland acinar cells, stimulating the secretion of proteins 20. However, it is not known whether activation of these receptors promotes an increase in tear secretion.…”

Section: Discussionmentioning

confidence: 99%

Short-term effect of topical antiglaucoma medication on tear-film stability, tear secretion, and corneal sensitivity in healthy subjects

Terai¹,

Müller-Holz²,

Spoerl³

et al. 2011

OPTH

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Background:The purpose of this study was to investigate the short-term effect of topical antiglaucoma medication on tear-film stability, tear secretion, and corneal sensitivity in healthy subjects.Methods:In this prospective, double-blind crossover trial, break-up time and basal secretion (Jones test) were measured 60 minutes before, and 30, 60, and 90 minutes after topical antiglaucoma drop application in 30 healthy subjects. Corneal sensitivity was measured 60 minutes before, and five, 10, and 15 minutes after drop application using a Cochet–Bonnet esthesiometer.Results:Reduction of break-up time in the latanoprost group was −23.8% after 30 minutes (P = 0.21), −26.7% after 60 minutes (P = 0.03) and −51.4% after 90 minutes (P ≤ 0.003), which was statistically significant. Reduction of break-up time in all other treatment groups was not statistically significant. The Jones test revealed a significant reduction of basal secretion after application of brimonidine (−17.8%, P = 0.002; −22.5%, P < 0.001; −30.5%, P < 0.001), followed by apraclonidine (−10%, P = 0.06; −20.1%, P = 0.02; −22.1%, P = 0.002), latanoprost (−2.4%, P = 0.64; −18.6%, P = 0.001; −20.1%, P = 0.001) and dorzolamide (−0.5%, P = 0.9; 14.3%, P = 0.018; −17.3%, P = 0.004) at 30, 60, and 90 minutes after drop application. Reduction of basal secretion in all other treatment groups was not statistically significant.Conclusion:Latanoprost showed the most statistically significant reduction in break-up time, and brimonidine showed the most significant reduction in basal secretion of all the glaucoma medications used in this study. In conclusion, our data may be helpful for treatment decisions in glaucoma patients who also suffer from ocular surface problems.

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Modification of stimulated lacrimal gland flow by sympathetic nerve impulses in rabbit

Cited by 56 publications

References 0 publications

Lacrimal gland electrolyte and water secretion in the rabbit: localization and role of (Na+ + K+)‐activated ATPase.

Lacrimal gland electrolyte and water secretion in the rabbit: localization and role of (Na+ + K+)‐activated ATPase.

TFOS DEWS II pain and sensation report

Short-term effect of topical antiglaucoma medication on tear-film stability, tear secretion, and corneal sensitivity in healthy subjects

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