Modification of radiation resistance of Bacillus spores by water

Todoriki, Setsuko; Furuta, Mamoru; Nagai, Toshirou; Hayashi, Toru

doi:10.1016/s0969-806x(99)00420-x

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…While most microorganisms are sensitive to elevated radiation levels, certain extremophiles, such as Deinococcus radiodurans (Mattimore and Battista, 1996), and spore-formers exhibit remarkable resistance to radiation (Bruhn et al 1999;Natalia et al 2009). Notably, some microorganisms frequently present in bentonite, such as Bacillus and Acinetobacter, also exhibit high radiation resistance (Stroes-Gascoyne et al 1995; Todoriki et al 2000). The simultaneous application of heat and radiation can stimulate biphasic survival responses, indicating synergistic effects between heat and radiation (Stroes-Gascoyne and West 1997)…”

Section: Introductionmentioning

confidence: 99%

Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: Implications for deep geological repository

Bartak,

Šachlová,

Kašpar

et al. 2024

Preprint

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Bentonite is an integral part of the engineered barrier system (EBS) in deep geological repositories (DGRs) for nuclear waste, but its indigenous microorganisms may jeopardize long-term EBS integrity. To predict microbial activity in DGRs, it is essential to understand microbial reactions to the early hot phase of DGR evolution. Two bentonites (BCV and MX-80) with varied bentonite/water ratios and saturation levels (compacted to 1600 kg.m-3 dry density/powder/suspension), were subjected to heat (90°C or 150°C) and irradiation (0.4 Gy.h-1) in the long-term experiments (up to 18 months). Molecular-genetic, microscopic, and cultivation-based techniques assessed microbial survivability. Exposure to 90°C and 150°C notably diminished microbial viability, irrespective of bentonite form, with negligible impacts from irradiation or sample type compared to temperature. Bentonite powder samples exhibited microbial recovery after 90°C heating for up to 6 months but not 12 months in most cases; exposure to 150°C had an even stronger effect. Further long-term experiments at additional temperatures combined with mathematical prediction of temperature evolution in DGR are recommended to validate the possible evolution and spatial distribution of microbially depleted zones in bentonite buffer around waste canister and refine predictions of microbial effects over time in the DGR.

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Section: Introductionmentioning

confidence: 99%

Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: Implications for deep geological repository

Bartak,

Šachlová,

Kašpar

et al. 2024

Preprint

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Decontamination of Biological Warfare Agents

Niederwöhrmeier¹,

Richardt²

2008

Decontamination of Warfare Agents

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Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: implications for deep geological repository

Bartak,

Šachlová,

Kašpar

et al. 2024

World J Microbiol Biotechnol

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Bentonite is an integral part of the engineered barrier system (EBS) in deep geological repositories (DGR) for nuclear waste, but its indigenous microorganisms may jeopardize long-term EBS integrity. To predict microbial activity in DGRs, it is essential to understand microbial reactions to the early hot phase of DGR evolution. Two bentonites (BCV and MX-80) with varied bentonite/water ratios and saturation levels (compacted to 1600 kg.m− 3 dry density/powder/suspension), were subjected to heat (90–150 °C) and irradiation (0.4 Gy.h− 1) in the long-term experiments (up to 18 months). Molecular-genetic, microscopic, and cultivation-based techniques assessed microbial survivability. Exposure to 90 °C and 150 °C notably diminished microbial viability, irrespective of bentonite form, with negligible impacts from irradiation or sample type compared to temperature. Bentonite powder samples exhibited microbial recovery after 90 °C heating for up to 6 months but not 12 months in most cases; exposure to 150 °C had an even stronger effect. Further long-term experiments at additional temperatures combined with the mathematical prediction of temperature evolution in DGR are recommended to validate the possible evolution and spatial distribution of microbially depleted zones in bentonite buffer around the waste canisters and refine predictions of microbial effects over time in the DGR.

show abstract

Modification of radiation resistance of Bacillus spores by water

Cited by 3 publications

References 4 publications

Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: Implications for deep geological repository

Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: Implications for deep geological repository

Decontamination of Biological Warfare Agents

Dramatic loss of microbial viability in bentonite exposed to heat and gamma radiation: implications for deep geological repository

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