Modification of Phalaenopsis Metabolism by Genetic Engineering

Chen, Y.-C.; Yang, Yiru; Chen, Y.-H.; Chao, Yuzhen; Chang, Lei; Chen, Y.-W.

doi:10.17660/actahortic.2010.878.61

Cited by 5 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results showed variation in genome size within 18 Phalaenopsis species. Further, new commercial hybrids were produced via chromosome doubling [21] and can be used for the comparative assessment of DNA content, providing insights into the evolution of Phalaenopsis orchids and assisting the orchid breeders in the selection of parent varieties for the hybridization process. Further, to understand the relationship and molecular characterization of different species, sequence-based microsatellite markers were used [205].…”

Section: Computational and Omics Approaches In Orchid Biologymentioning

confidence: 99%

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Tiwari,

Sharma,

Bose

et al. 2024

Horticulturae

View full text Add to dashboard Cite

Orchids constitute the largest and most diverse group of flowering plants and are classified in the family Orchidaceae. Exhibiting significance as the most exotic and ubiquitous flowering plant, the cultivation of orchids on a commercial level is gaining momentum worldwide. In addition to its ornamental and aesthetic value, the orchid industry has successfully generated employment for people in developing countries. Recent advances in biotechnological interventions in orchids have substantially contributed to the development of exotic varieties with novel traits, not to forget the inputs of traditional plant breeding methods and tissue culture approaches. In addition, the scientific developments in orchid biology have remarkably bridged the knowledge gaps in areas of orchid classification, phytochemistry, and cultivation strategies. This has facilitated the commercialization of novel varieties, opening new avenues in the orchid industry, and their global marketing as cut flowers and artificially propagated plants. Orchids constitute the first floriculture crops that revolutionized the orchid industry; however, they also hold several challenges in the natural propagation and conservation of several species that are on the verge of extinction. International organizations like CITES have come forward to address challenges associated with illegal global trade and indiscriminate use of orchid varieties, aiming for conservation and legal commercial goals. This thematic review is one-of-a-kind in providing comprehensive insights into the emerging momentum of orchid biology and how its globalization projects to considerably impact the orchid industry in the coming times. However, it is imperative to understand the challenges in the cultivation and conservation of orchid varieties and ensure legislative guidelines both on domestic and global levels to ensure a multipronged approach to the conservation and commercialization of orchids.

show abstract

Section: Computational and Omics Approaches In Orchid Biologymentioning

confidence: 99%

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Tiwari,

Sharma,

Bose

et al. 2024

Horticulturae

View full text Add to dashboard Cite

show abstract

“…correlate with their chromosome sizes and also observed highest degree of endoreduplication in P. equestris leaves. Through chromosomal doubling new commercial hybrids were produced in Phalaenopsis (Chen et al 2010b) and these hybrids can be used for comparative analyses of DNA content on evolution of Phalaenopsis or help to the orchid breeders and molecular geneticists for the selection of parental varieties for hybridization. Kao et al (2001) analyzed karyotypes of nine representative Phalaenopsis species and D. pulcherrima and reported genome size and the amount of constitutive heterochromatin (CH) among the species.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Phalaenopsis Orchid Improvement using Omics Approaches

Balilashaki

Zakizadeh

Olfati

et al. 2019

Plant Tissue Cult. & Biotech.

View full text Add to dashboard Cite

With recent advances in high-throughput sequencing (HTS) technologies to improve plants, there is a need to release orchid specific genomic resources and platforms that are crucial for managing omics elements in systematic manner. Authors provide details about the recent developments in biotechnological techniques, genomics, transcriptomics, proteomics, metabolomics and their applications for the industrial production, propagation, conservation and manipulation of Phalaenopsis orchid. Plant Tissue Cult. & Biotech. 29(1): 133-149, 2019 (June)

show abstract

“…So far, the two most-often used host orchid tissues, protocorms and PLBs have been genetically transformed equally well. PLBs are presumed to be genetically uniform and can be induced efficiently from various somatic tissues including flower stalks, young leaves and stem segments (Chen et al 2010 ; Yee et al 2008 ). However, protocorm transformations have been proposed to be simpler than subculture of PLBs (Mishiba et al 2005 ; Semiarti et al 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Efficient and heritable transformation of Phalaenopsis orchids

et al. 2016

View full text Add to dashboard Cite

Background Phalaenopsis orchid (Phal. orchid) is visually attractive and it is important economic floriculture species. Phal. orchids have many unique biological features. However, investigation of these features and validation on their biological functions are limited due to the lack of an efficient transformation method.ResultsWe developed a heritable and efficient Agrobacterium- mediated transformation using protocorms derived from tetraploid or diploid Phal. orchids. A T-DNA vector construct containing eGFP driven by ubiquitin promoter was subjected to transformation. An approximate 1.2–5.2 % transformation rate was achieved. Genomic PCR confirmed that hygromycin selection marker, HptII gene and target gene eGFP were integrated into the orchid genome. Southern blotting indicated a low T-DNA insertion number in the orchid genome of the transformants. Western blot confirmed the expression of eGFP protein in the transgenic orchids. Furthermore, the GFP signal was detected in the transgenic orchids under microscopy. After backcrossing the pollinia of the transgenic plants to four different Phal. orchid varieties, the BC1 progenies showed hygromycin resistance and all surviving BC1 seedlings were HptII positive in PCR and expressed GFP protein as shown by western blot.ConclusionsThis study demonstrated a stable transformation system was generated for Phal. orchids. This useful transformation protocol enables functional genomics studies and molecular breeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-016-0146-6) contains supplementary material, which is available to authorized users.

show abstract

Modification of Phalaenopsis Metabolism by Genetic Engineering

Cited by 5 publications

References 0 publications

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Advances in Orchid Biology: Biotechnological Achievements, Translational Success, and Commercial Outcomes

Recent Advances in Phalaenopsis Orchid Improvement using Omics Approaches

Efficient and heritable transformation of Phalaenopsis orchids

Contact Info

Product

Resources

About