“…Free VCR, VCR/LPNs, and FA-VCR/LPNs (each contained 10 mg/kg of VCR) were intravenous injected via the tail vein, respectively. 26 Blood samples (300 µL) were collected at pre-determined times (0.25, 0.5, 0.75, 1, 2, 4, 6, 8, 12, 24, 48, and 72 h) in polypropylene microcentrifuge tubes, centrifuged at 15,000 rpm for 5 minutes to isolate the plasma, and stored at −80°C and subsequently analyzed by HPLC using the method mentioned in the “Drug encapsulation and loading efficiency” section.…”