Modification of oxygen evolving center by Tris-washing

Abstract

Tris-washing inhibits the O2-evolving center of chloroplasts and their particles specifically and reversibly, and it was applied to many investigations on O2-evolving center and PS II reaction center. In this review are introduced the various photosynthetic investigations in which Tris-washing was applied and are also discussed briefly on the site and the mechanism of Tris-inactivation, properties of P680 and Z, characteristic change in fluorescence and delayed light emission, and reactivation of O2-evolving center by DCPIP.H2-treatment and photo-reactivation of Tris-washed chloroplasts and their particles.

Oxygen and ROS in Photosynthesis

“…The E m values of the three forms of cyt b559 are (see review [239]): The ratio between the forms is variable and depends on isolation procedure. For example, modification of the donor side of PSII by removal of the Mn-cluster leads to the conversion of the HP form to the LP form [241]. In intact chloroplasts, the ratio of HP to LP forms was found to be 58 to 31, with respective redox potentials of 383 and 77 mV [242].…”