Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers

Reiter, Martina; Walf, Vanessa M.; Christians, Arne; Pfaffl, Michael W.; Meyer, H. H. D.

doi:10.1016/j.aca.2006.10.049

Cited by 48 publications

(51 citation statements)

References 34 publications

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“…As a consequence, the positive identification of growth-promoter treated animals occurs infrequently which is at variance with evidence that there is continued sporadic use of illicit growth-promoters throughout Europe [5]. The use of biological evidence from a range of sources has been proposed as a potential additional tool to improve the effectiveness of procedures to detect illicit growth-promoter use [6][7][8]. An indirect approach based on detecting deviant or unusual perturbations in the circulating levels of plasma analytes has recently been shown to demonstrate potential [9].…”

Section: Introductionmentioning

confidence: 98%

Biosensor-based detection of reduced sex hormone-binding globulin binding capacities in response to growth-promoter administrations

Mooney¹,

Bergwerff

Meeuwen

et al. 2009

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 98%

Biosensor-based detection of reduced sex hormone-binding globulin binding capacities in response to growth-promoter administrations

Mooney¹,

Bergwerff

Meeuwen

et al. 2009

Analytica Chimica Acta

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“…Second, to identify a set of differentially expressed genes in treated animals compared with controls. Gene expression might then be used as an indirect biological marker (57,74) to screen for illicit use of corticosteroids in samples collected at slaughterhouses.…”

mentioning

confidence: 99%

Expression profiling of skeletal muscle in young bulls treated with steroidal growth promoters

Carraro¹,

Ferraresso²,

Cardazzo³

et al. 2009

Physiological Genomics

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Dexamethasone (Dex), alone or in association with estrogens, is often illegally administered per os at very low dosage as a growth promoter in beef cattle, with effects that are opposite to the muscle wasting and atrophy induced by repeated administration at therapeutic dosages. In vitro and in vivo studies have investigated the catabolic effects of Dex at therapeutic doses on skeletal muscle, demonstrating an increase in the expression of GDF8 (myostatin) gene, a well-known negative regulator of skeletal muscle mass, in a dose-dependent way. This suggested a direct role of myostatin in Dex-induced muscle wasting. In the present study, an oligonucleotide microarray platform was used to compare expression profiles of beef cattle muscle in animals treated with either Dex or Dex plus 17-β estradiol (Estr) administered at subtherapeutic dosage, against untreated controls. Data analysis demonstrates that the expression profiles were strongly affected by Dex treatment with hundreds of genes upregulated with relevant fold-change, whereas seven genes were downregulated including the myostatin gene. On the contrary, the number of differentially regulated genes was lower in response to the addition of Estr to the Dex treatment. Differentially regulated genes were analyzed to describe the effects of these treatments on muscle physiology, highlighting the importance of specific pathways (e.g., Wnt or cytokine signaling) and cellular processes (e.g., cell shape and motility). Finally, the observed differences in the expression profile will allow the development of indirect bio-markers to detect illegal Dex treatments in beef cattle using quantitative RT-PCR.

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“…In uterine tissue, 13 significantly regulated genes could be identified, whereas the expression changes were up to 6-fold. [25] These results again indicate that hormone-dependent tissues are potential targets for the identification of gene expression biomarkers to screen for the misuse of anabolic steroids.…”

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tmentioning

confidence: 86%

“…[26] Comparing these results with those published by Reiter et al, it can be observed, that AR and Caspase 3 were also significantly regulated by Finaplix W indicating that these genes could act as first biomarkers in uterine tissue for treatment with Trenbolone Acetate. [25] Regarding the results obtained in ovary, where 22 genes were significantly regulated, it can be concluded that ovary will be a more potential organ for the identification of gene expression changes in order to find biomarkers for the abuse of anabolic steroids. [26] Another new approach in the same animal trial was the quantification of gene expression in vaginal smear containing vaginal epithelial cells.…”

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tmentioning

confidence: 99%

“…As already described above, Reiter et al examined the expression of different genes in hind limb muscle and neck muscle upon treatment with MGA, Finaplix W , and Ralgro W , whereas they found eight and eleven significantly regulated genes, respectively. [25] De Jager et al used microarray analysis to identify differently expressed genes in longissimus dorsi muscle after implantation of Revalor-H W (Trenbolone Acetate plus Estradiol). In this experiment, 121 out of 16 944 quantified genes were differentially expressed.…”

Section: Efforts Made In the Analysis Of The Transcriptome In Order Tmentioning

confidence: 99%

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The physiological way: Monitoring RNA expression changes as new approach to combat illegal growth promoter application

Riedmaier

Pfaffl

Meyer

2012

Drug Testing and Analysis

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The use of growth-promoting agents in food-producing animals is forbidden in the European Union (EU). Therefore a strict control programme has been developed, detecting residues of all known growth-promoting agents using chromatographical methods in combination with mass spectrometry or immunoassays. New designed xenobiotic substances or hormone cocktails are difficult to identify with these methods and therefore the development of new sensitive test methods is important.A promising indirect approach is the detection of physiological effects of the administered growth promoters on the molecular level using 'omic' technologies. The analysis of the transcriptome on mRNA and miRNA level and thereby identifying biomarkers for the use of anabolic agents is one possible strategy for developing a new screening method. This paper describes the technologies available for gene expression profiling and summarizes the efforts made in the analysis of the transcriptome in order to identify potential gene expression biomarkers for the use of growth promoters in cattle.

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Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers

Cited by 48 publications

References 34 publications

Biosensor-based detection of reduced sex hormone-binding globulin binding capacities in response to growth-promoter administrations

Biosensor-based detection of reduced sex hormone-binding globulin binding capacities in response to growth-promoter administrations

Expression profiling of skeletal muscle in young bulls treated with steroidal growth promoters

The physiological way: Monitoring RNA expression changes as new approach to combat illegal growth promoter application

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