Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol

Abe, Takahito; Kawahara-Miki, Ryouka; Hara, Tomotaka; Noguchi, Toshihiro; Hayashi, Takeshi; Shirasuna, Koumei; Kuwayama, Takehito; Iwata, Hisataka

doi:10.1262/jrd.2016-182

Cited by 24 publications

(22 citation statements)

References 34 publications

(41 reference statements)

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“…High concentration (2.0 μM) significantly reduced the total blastocyst formation rate compared to the control or low concentrations (Table 1). This was consistent with previous studies indicating that high concentrations of resveratrol might have negative effects on embryonic development [25,26]. However, since different results have been reported, differences in the fertilization method, the culture medium used, and the mice species might be associated with such discrepancies [10].…”

Section: Discussionsupporting

confidence: 92%

Effects of resveratrol, granulocyte-macrophage colony-stimulating factor or dichloroacetic acid in the culture media on embryonic development and pregnancy rates in aged mice

Yoon

Juhn

Jung

et al. 2020

Aging

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The success rate of assisted reproductive technology is closely correlated with maternal age. Reproductive aging pathologies are frequently caused by impaired DNA repair, genomic instability, and mitochondrial dysfunction. Several reports have shown that resveratrol can prevent age-related diseases by improving mitochondrial function. Improved blastocyst development and mitochondrial output by dichloroacetic acid (DCA) supplementation were reported in aged mice. Granulocyte-macrophage colony-stimulating factor (GM-CSF) has significant effects on implantation rates in women with previous miscarriages. Therefore, this study was conducted to observe how those compounds influence the developmental and the reproductive potential of aged oocytes. BDF1 female mice at 58-62 weeks old were used for this study. MII oocytes were fertilized and cultured in MRC media supplemented with or without resveratrol (0.5 µM), GM-CSF (2 ng/ml) or DCA (1.0 mM). The addition of resveratrol, GM-CSF or DCA tended to increase blastocyst development and pregnancy rates. Supplementation with resveratrol significantly increased the pregnancy and implantation rates (p < 0.05). Moreover, resveratrol decreased reactive oxygen species production and increased mitochondrial membrane potential. These results suggest that the addition of resveratrol can increase pregnancy outcomes in women of advanced maternal age.

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Section: Discussionsupporting

confidence: 92%

Effects of resveratrol, granulocyte-macrophage colony-stimulating factor or dichloroacetic acid in the culture media on embryonic development and pregnancy rates in aged mice

Yoon

Juhn

Jung

et al. 2020

Aging

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“…In the present study, the supplementation of the embryo culture medium with resveratrol enhanced the developmental rate till the blastocyst stage. Previous reports have highlighted the beneficial effects of resveratrol on the embryonic development of porcine and bovine embryos had been reported previously [ 22 , 37 ]. In the present study, the incubation of 8-cell stage bovine embryos in the resveratrol-containing medium resulted in an increase in the expression level of SIRT1 in the resultant blastocysts [ 22 ].…”

Section: Discussionmentioning

confidence: 93%

“…Previous reports have highlighted the beneficial effects of resveratrol on the embryonic development of porcine and bovine embryos had been reported previously [ 22 , 37 ]. In the present study, the incubation of 8-cell stage bovine embryos in the resveratrol-containing medium resulted in an increase in the expression level of SIRT1 in the resultant blastocysts [ 22 ]. Furthermore, resveratrol had no effect on the mitochondrial number and total cell number of blastocysts.…”

Section: Discussionmentioning

confidence: 93%

“…Resveratrol was diluted in ethanol (vehicle) and added to the medium (2,000×) at a concentration of 0.5 μM. This concentration of resveratrol was selected based on the previous studies using bovine early embryos and blastocysts [ 22 , 23 ]. Embryos cultured in the medium containing ethanol without resveratrol were used as the control groups.…”

Section: Methodsmentioning

confidence: 99%

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Resveratrol enhances the clearance of mitochondrial damage by vitrification and improves the development of vitrified-warmed bovine embryos

Hara¹,

Kin²,

Aoki³

et al. 2018

PLoS ONE

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The present study investigated the vitrification-induced deterioration of mitochondrial functions that may reduce the developmental ability of post-warming bovine embryos. In addition, the effect of supplementation of the culture medium with resveratrol on the mitochondrial functions and post-warming embryonic development was examined. Two days after in vitro fertilization, embryos with 8–12 cells (referred to hereafter as 8-cell embryos) were vitrified and warmed, followed by in vitro incubation for 5 days in a culture medium containing either the vehicle or 0.5 μM resveratrol. Vitrification reduced embryonic development until the blastocyst stage, reduced the ATP content of embryos, and impaired the mitochondrial genome integrity, as determined by real-time polymerase chain reaction. Although the total cell number and mitochondrial DNA copy number (Mt-number) of blastocysts were low in the vitrified embryos, the Mt-number per blastomere was similar among the blastocysts derived from fresh (non-vitrified) and vitrified-warmed embryos. Supplementation of the culture medium with resveratrol enhanced the post-warming embryonic development and reduced the Mt-number and reactive oxygen species level in blastocysts and blastomeres without affecting the ATP content. An increase in the content of cell-free mitochondrial DNA in the spent culture medium was observed following cultivation of embryos with resveratrol. These results suggested that vitrification induces mitochondrial damages and that resveratrol may enhance the development of post-warming embryos and activates the degeneration of damaged mitochondria, as indicated by the increase in the cell-free mitochondrial DNA content in the spent culture medium and the decrease in the Mt-number of blastocysts and blastomeres.

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“…Consistent with these reports, we found that post-warming blastocysts had lower ATP content than their fresh counterparts. Furthermore, the ATP content in frozen-thawed embryos were still low after 24 hr of incubation (1.31 pM, Figure 5-A) compared with fresh blastocysts examined under the same conditions (1.77 pM, Abe et al, 2017), in addition mitochondrial copy number in the frozen-thawed embryos were also low compared with those in fresh embryos (Figure 3).…”

Section: Discussionmentioning

confidence: 92%

Effects of resveratrol treatment on mitochondria and subsequent embryonic development of bovine blastocysts cryopreserved by slow freezing

Hayashi

Kansaku

Abe

et al. 2019

Animal Science Journal

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This study evaluated the effects of cryopreservation by slow freezing on the mitochondrial function, DNA integrity, and developmental ability of bovine embryos and examined whether resveratrol treatment of the frozen‐thawed blastocysts improved embryonic viability. In vitro produced bovine embryos were subjected to slow freezing. After thawing, the ATP content and mitochondrial DNA integrity (mtDNA), determined by real‐time PCR targeting short and long mitochondrial sequences, was found to be lower in frozen‐thawed embryos than in fresh embryos, and mtDNA copy number was significantly reduced during the 24‐hr incubation post warming. Furthermore, immunostaining against double‐strand DNA revealed DNA damage in frozen‐thawed embryos. When frozen‐thawed embryos were incubated in the medium containing 0.5 µM resveratrol, SIRT1 expression, and survival rate of the embryos significantly improved compared with the vehicle‐treated embryos. In addition, cell‐free mtDNA content in medium was higher in case of resveratrol‐treated embryos than of vehicle‐treated embryos. In conclusion, slow freezing affects mitochondrial integrity and function in the blastocysts. In the frozen‐thawed embryos, mitochondria were removed during post‐thawing incubation and resveratrol enhanced the process, resulting in improved survivability of the embryos.

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Modification of mitochondrial function, cytoplasmic lipid content and cryosensitivity of bovine embryos by resveratrol

Cited by 24 publications

References 34 publications

Effects of resveratrol, granulocyte-macrophage colony-stimulating factor or dichloroacetic acid in the culture media on embryonic development and pregnancy rates in aged mice

Effects of resveratrol, granulocyte-macrophage colony-stimulating factor or dichloroacetic acid in the culture media on embryonic development and pregnancy rates in aged mice

Resveratrol enhances the clearance of mitochondrial damage by vitrification and improves the development of vitrified-warmed bovine embryos

Effects of resveratrol treatment on mitochondria and subsequent embryonic development of bovine blastocysts cryopreserved by slow freezing

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