Modification of Metoclopramide GLC Assay: Application to Human Biological Specimens

Tam, Y K; Axelson, James E.; Ongley, R. C.

doi:10.1002/jps.2600681015

Cited by 19 publications

(2 citation statements)

References 4 publications

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“…Chromatographic methods have been described for the quantitative determination of metoclopramide in biological fluids. These include gas chromatography (RossLee et al, 1980;Tam et al, 1979) and high performance liquid chromatography (Graffner et al, 1979;Bateman et al, 1981;Vergin et al, 1983;El-Sayed et al, 1994;Dobrev et al, 1995). Most of the HPLC determinations, with two exceptions (Vergin et al, 1983;El-Sayed et al, 1994), were performed on a normal silica stationary phase and all utilized UV detection.…”

Section: Introductionmentioning

confidence: 98%

RP‐HPLC method with electrochemical detection for the determination of metoclopramide in serum and its use in pharmacokinetic studies

Lamparczyk¹,

Chmielewska²,

Konieczna³

et al. 2001

Biomedical Chromatography

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A rapid and sensitive reversed-phase high performance liquid chromatographic method has been developed for the determination of metoclopramide in serum. The assay was performed after single extraction with ethyl ether using methyl parahydroxybenzoate as internal standard. Chromatographic separations were performed on C(18) stationary phase with a mobile phase composed of methanol-phosphate buffer pH 3 (30:70 v/v). Analytes were detected electrochemically. The quantification limit for metoclopramide in serum was 2 ng mL(-1). Linearity of the method was confirmed in the range of 5-120 ng mL(-1) (correlation coefficient 0.9998). Within-day relative standard deviations (RSDs) ranged from 0.3 to 5.5% and between-day RSDs from 0.8 to 6.0%. The analytical method was successfully applied for the determination of pharmacokinetic parameters after ingestion of 10 mg dose of metoclopramide. Studies were performed on 18 healthy volunteers of both sexes.

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Section: Introductionmentioning

confidence: 98%

RP‐HPLC method with electrochemical detection for the determination of metoclopramide in serum and its use in pharmacokinetic studies

Lamparczyk¹,

Chmielewska²,

Konieczna³

et al. 2001

Biomedical Chromatography

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“…For this reason use of much more sensitive method is necessary. Trace amounts need instrumental techniques, such as derivative gas chromatography [59][60][61][62][63][64][65][66][67][68]. Separately described HPLC-MS method reported about significantly lower quantitation limit of hydrochlorothiazide [29].…”

Section: Introductionmentioning

confidence: 99%

Sensitive quantification of chosen drugs by reversed-phase chromatography with electrochemical detection at a glassy carbon electrode

Chmielewska

Konieczna

Plenis

et al. 2006

Journal of Chromatography B

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High performance liquid chromatographic determination of metoclopramide in plasma and urine and its application to biopharmaceutical investigations

Nieder¹,

Jaeger²

1987

J. High Resol. Chromatogr.

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SummaryAnoptimized HPLC method forthe quantificationof metoclopramide (MCP) in human plasma and urine is described. MCP and internal standard are extracted from alkalinized substrate into diethyl ether and back-extracted into dilute acid. The analytes are separated with a ternary mobile phase at cyanopropyl-silica and detected at 312 nm (UV detection). The lower limit of quantification is 0.5 ng/ml in plasma and 50 n g h l in urine. Optimization of extraction, chromatography, and detection is discussed. The method is selective to numerous common drug substances with excellent accuracy and precision data. After validation, the method is applied to the samples of a pharmacokinetic study. Pharmacokinetic parameters indicate the need for a sophisticated method as tool for optimization of metoclopramide formulations.

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Modification of Metoclopramide GLC Assay: Application to Human Biological Specimens

Cited by 19 publications

References 4 publications

RP‐HPLC method with electrochemical detection for the determination of metoclopramide in serum and its use in pharmacokinetic studies

RP‐HPLC method with electrochemical detection for the determination of metoclopramide in serum and its use in pharmacokinetic studies

Sensitive quantification of chosen drugs by reversed-phase chromatography with electrochemical detection at a glassy carbon electrode

High performance liquid chromatographic determination of metoclopramide in plasma and urine and its application to biopharmaceutical investigations

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