Modification of membrane properties of erythrocytes by PGI2

Pintigny, D; Wautier, Jean‐Luc; Wautier, Marie‐Paule; Caen, Jacques P.

doi:10.1016/0049-3848(89)90130-8

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…The mean 6kPGF1, level measured in these plasma samples was 0.18 + 0.06 (mean 2 SE, n = 18) pmol1mL. Although all of these samples were assayed in triplicate volumes (25,50, and 100 pL), only seven out of 18 of these samples had measurable eicosanoid levels with 50 p L plasma, whereas no 6kPGFl, was detected when 25 p L plasma were used in the assay. Although the variability among the duplicates generated using multiple aliquots of the same volume was within 5%, there was no linearity between the volume of plasma assayed versus 6kPGFl, levels measured.…”

Section: Comparison Of Plasma 6kpgf Levels Measured With Direct Assmentioning

confidence: 91%

“…The role of cyclooxygenase metabolites including prostacyclin on red cell-endothelial cell adherence has previously been evaluated with conflicting results. Wautier and co-workers found that although exogenously provided prostacyclin stimulated adherence, inhibition of endogenous prostacyclin production by flurbiprofen (a cyclooxygenase inhibitor), had no effect on the adherence process (25,26). In contrast, other investigators have demonstrated that iloprost (a stable analog of prostacyclin) inhibited the adherence process (27).…”

Section: Referencementioning

confidence: 99%

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Sickle Cell Vaso-occlusive Crisis Is Associated with Abnormalities in the Ratio of Vasoconstrictor to Vasodilator Prostanoids

1995

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Plasma levels of 6-keto-prostaglandin F1 alpha (6kPGF1 alpha) and thromboxane (Tx) B2 have been assessed in sickle cell disease (SCD) with discrepant results. Inasmuch as direct measurement of plasma prostanoids is fraught with the problem of interfering substances, we assessed plasma 6kPGF1 alpha and TxB2 levels in patients with SCD by RIA after extraction of eicosanoids and separation by HPLC. We demonstrate that the 6kPGF1 alpha and TxB2 levels in children with SCD in steady state as well as in vaso-occlusive crisis (VOC) are significantly lower when compared with those from age-matched controls. The VOC plasma 6kPGF1 alpha and TxB2 levels were, however, significantly elevated when compared with those from children in steady state. Changes similar to those noted with unpaired plasma samples were also observed when paired steady state and VOC plasmas from the same patients were assessed. The ratio of TxB2 to 6kPGF1 alpha was, however, significantly elevated in patients with SCD in crisis when compared with eicosanoid ratios obtained during steady state. In an attempt to understand whether the abnormality in 6kPGF1 alpha was due to an impairment in endothelial cell prostacyclin-regenerating ability, we compared the ability of plasma from controls and children with SCD to activate arachidonic acid (AA) release and prostacyclin production by [14C]AA-prelabeled bovine aortic endothelial cells. Our results suggest that the decreased 6kPGF1 alpha levels in plasma from children with SCD was not due to an effect on substrate AA release but rather a modulatory effect of sickle plasma components on endothelial cell cyclooxygenase activity.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Comparison Of Plasma 6kpgf Levels Measured With Direct Assmentioning

confidence: 91%

Section: Referencementioning

confidence: 99%