Modification of Intradermal Delayed Hypersensitivity by Components of Leukocyte Dialysates

Xenogeneic immunotherapy of transplantable guinea pig hepatoma with primate leukocyte lysates

“…Modification of dermal delayed hypersensitivity with dialyzable human leukocyte lysate components has been reported by Gottlieb et al [10]. These investigators observed that dialysates had nonspecific dermal inflammatory effects in the absence of antigen, as well as dialysate substances that appeared to amplify donor reactivity to specific antigen [10].…”

“…These investigators observed that dialysates had nonspecific dermal inflammatory effects in the absence of antigen, as well as dialysate substances that appeared to amplify donor reactivity to specific antigen [10]. Mischak and Spitler [20] reported that nonspecific human leukocyte dialysates were able to potentiate delayed skin responses to a chemically defined antigen in guinea pigs.…”

“…Other investigators have reported on the nonspecific effects of leukocyte dialysates obtained from sensitized cells [8,10,37]. For example, Waxman et al [37] reported that marmoset lymphocytes treated with dialyzed leukocyte extracts from PPD-positive donors released MIF after incubation with KLH.…”

Regulation of Lymphocyte Transformation by Components Identified in Ultrafiltrates Prepared from Leukocyte Extracts

“…Con versely, other components in DLE suppress delayedtype hypersensitivity reactions [9,14,15]. The aug menter is recoverable in association with the major fluram peak (G-2 to G-3 fraction) by gel filtration of the S* fraction (MW < 5,000) on a Sephadex G-10 col umn.…”