Modification of intestinal flora with multispecies probiotics reduces bacterial translocation and improves clinical course in a rat model of acute pancreatitis

Minnen, L. Paul van; Timmerman, Harro M.; Lutgendorff, Femke; Verheem, André; Harmsen, W.S.; Konstantinov, Sergey R.; Smidt, Hauke; Visser, Maarten R.; Rijkers, Ger T.; Gooszen, Hein G.; Akkermans, L.M.A.

doi:10.1016/j.surg.2006.10.007

Cited by 115 publications

(92 citation statements)

References 54 publications

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“…Surgical procedures were performed as described previously (26). Briefly, all procedures were performed under general anesthesia using a combination of 2% isoflurane gas and 0.3 ml 10% buprenorphine intramuscularly administered (Temgesic; Reckitt Benckiser Healthcare Ltd., Hull, United Kingdom).…”

Section: Animalsmentioning

confidence: 99%

“…A portion of pancreatic tissue was fixed in 4% formalin and analyzed histopathologically, using standard hematoxylin and eosin (H&E) staining. The histopathological severity of acute pancreatitis was assessed based on a scoring system modified from Schmidt et al (21) as previously described (26). Several aspects of (peri)pancreatic histopathology were assessed (peritonitis, edema, ductal pathology, inflammatory infiltrate, acinar cells, acinar dilatation, and hemorrhagic changes).…”

Section: Animalsmentioning

confidence: 99%

“…All tissue samples were weighed and processed immediately for quantitative and qualitative culturing of aerobic and anaerobic microorganisms in appropriate media as described previously (26). Tissue samples were homogenized in cysteine broth and cultured in 10-fold dilution series.…”

Section: Animalsmentioning

confidence: 99%

“…We have previously shown in an experimental rat model for acute pancreatitis that prophylactic administration of a mixture of probiotic bacterial species resulted in reduced bacterial translocation to the pancreas and other extraintestinal organs, improved disease outcome, and reduced late mortality of acute pancreatitis (26). Although the effects of the probiotic therapy were highly significant, not all treated animals were protected from the adverse consequences of experimental acute pancreatitis.…”

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confidence: 99%

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Correlation between Protection against Sepsis by Probiotic Therapy and Stimulation of a Novel Bacterial Phylotype

Gerritsen

Timmerman

Minnen

et al. 2011

Appl Environ Microbiol

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Prophylactic probiotic therapy has shown beneficial effects in an experimental rat model for acute pancreatitis on the health status of the animals. Mechanisms by which probiotic therapy interferes with severity of acute pancreatitis and associated sepsis, however, are poorly understood. The aims of this study were to identify the probiotic-induced changes in the gut microbiota and to correlate these changes to disease outcome. Duodenum and ileum samples were obtained from healthy and diseased rats subjected to pancreatitis for 7 days and prophylactically treated with either a multispecies probiotic mixture or a placebo. Intestinal microbiota was characterized by terminal-restriction fragment length polymorphism (T-RFLP) analyses of PCRamplified 16S rRNA gene fragments. These analyses showed that during acute pancreatitis the host-specific ileal microbiota was replaced by an "acute pancreatitis-associated microbiota." This replacement was not reversed by administration of the probiotic mixture. An increase, however, was observed in the relative abundance of a novel bacterial phylotype most closely related to Clostridium lituseburense and referred to as commensal rat ileum bacterium (CRIB). Specific primers targeting the CRIB 16S rRNA gene sequence were developed to detect this phylotype by quantitative PCR. An ileal abundance of CRIB 16S rRNA genes of more than 7.5% of the total bacterial 16S rRNA gene pool was correlated with reduced duodenal bacterial overgrowth, reduced bacterial translocation to remote organs, improved pancreas pathology, and reduced proinflammatory cytokine levels in plasma. Our current findings and future studies involving this uncharacterized bacterial phylotype will contribute to unraveling one of the potential mechanisms of probiotic therapy.

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Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

Section: Animalsmentioning

confidence: 99%

mentioning

confidence: 99%

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Correlation between Protection against Sepsis by Probiotic Therapy and Stimulation of a Novel Bacterial Phylotype

Gerritsen

Timmerman

Minnen

et al. 2011

Appl Environ Microbiol

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“…75,76 A primeira parte do atual estudo foi desenvolvida como uma alternativa para a avaliação futura de translocação bacteriana utilizando o perfil de resistência bacteriana como um marcador. Este modelo consiste em modelo experimental simples e dispensa o uso de material radioativo e técnicas elaboradas e dispendiosas que envolvem biologia molecular.…”

Section: Discussionunclassified

Translocação bacteriana na isquemia-reperfusão hepática com e sem estase venosa intestinal: estudo experimental em ratos

Heijden¹

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Probiotics and Health Claims: Challenges for Tailoring their Efficacy

Chassard¹,

Grattepanche²,

Lacroix³

2010

Probiotics and Health Claims

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Modification of intestinal flora with multispecies probiotics reduces bacterial translocation and improves clinical course in a rat model of acute pancreatitis

Cited by 115 publications

References 54 publications

Correlation between Protection against Sepsis by Probiotic Therapy and Stimulation of a Novel Bacterial Phylotype

Correlation between Protection against Sepsis by Probiotic Therapy and Stimulation of a Novel Bacterial Phylotype

Translocação bacteriana na isquemia-reperfusão hepática com e sem estase venosa intestinal: estudo experimental em ratos

Probiotics and Health Claims: Challenges for Tailoring their Efficacy

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