Modification of in vitro immune lymphocyte-target cell interaction by some biologically active drugs

Brondz, B

doi:10.1016/0161-5890(73)90062-x

Cited by 7 publications

(8 citation statements)

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“…Monoclonal antibodies to various cellular antigens [33,42] or synthetic carbohydrate antigens [31,34] may have their fine specificities determined by immunodominant carbohydrate determinants. Attempts to resolve the controversy concerning recognition of carbohydrate determinants by T cells showed that alteration of the carbohydrate portion of an antigen (1) inhibited [5,30], (2) stimulated [7,29], or (3) had no effect [21,35] on the ability of T cells to recognize and respond to the antigen. These controversial results may have been due to the fact that most T cell responses appear to be directed against processed antigen in association with self-restricting antigens of the MHC [45].…”

Section: Discussionmentioning

confidence: 99%

T cell recognition of a tumor-associated glycoprotein and its synthetic carbohydrate epitopes: stimulation of anticancer T cell immunity in vivo

Henningsson

Selvaraj

MacLean

et al. 1987

Cancer Immunol Immunother

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The Thomsen, Friedenreich (TF) and Tn carbohydrate antigens are expressed on the vast majority of human adenocarcinomas and are associated with aggressive behavior of certain tumors. TF and Tn antigens are also expressed on certain murine cancer cell lines including TA3-Ha, a highly lethal, transplantable mammary adenocarcinoma. TF and Tn cancer-associated carbohydrate haptens were synthesized, conjugated to protein carriers and used to demonstrate that delayed-type hypersensitivity (DTH) effector T cells can specifically recognize and respond to carbohydrate determinants on the TA3-Ha tumor-associated glycoprotein, epiglycanin. The effector cells were shown to have the helper DTH phenotype (Lyt1+, Lyt2-, Thy1+) and it was demonstrated that they respond to specific carbohydrate determinants in an MHC-restricted fashion. These experiments provide the rationale for the use of synthetic tumor-associated glycoconjugates (S-TAGs) to stimulate anticancer T cell immunity. In support of this hypothesis, it was shown that preimmunization with the appropriate S-TAGs could provide a degree of protection against a subsequent tumor transplant and that antitumor effector Lyt1+, Lyt2- T cells could be generated in vitro using the appropriate S-TAGs as antigens.

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Section: Discussionmentioning

confidence: 99%

T cell recognition of a tumor-associated glycoprotein and its synthetic carbohydrate epitopes: stimulation of anticancer T cell immunity in vivo

Henningsson

Selvaraj

MacLean

et al. 1987

Cancer Immunol Immunother

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“…This augmentation was observed with four independent preparations, and may result from decreased repulsion between treated liposomes and negatively charged surfaces of pCTL. In fact, removal of negatively charged sialic acid by neuraminidase increases target cell sensitivity to CTL (7,8). Stability of the H-2K k molecule in either its native or deglycosylated state is underscored by maintenance of immunogenicity despite overnight incubation at 37 °C.…”

Section: Resultsmentioning

confidence: 99%

Carbohydrate moieties of major histocompatibility complex class I alloantigens are not required for their recognition by T lymphocytes.

Goldstein¹,

Mescher²

1985

The Journal of Experimental Medicine

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Cytotoxic T lymphocytes (CTL) have been implicated as effectors in allograft rejection and in control of viral infection. Induction of a cytolytic response requires T cell recognition of foreign major histocompatibility complex (MHC) class I glycoproteins, or syngeneic MHC antigens along with a foreign (i.e., viral) protein. This recognition process leads to proliferation and differentiation of precursor cells to effector CTL capable of lysing target cells that bear the same antigens. While class I oligosaccharide residues do not contribute to serologic determinants (1-4) their role in T cell recognition has remained unclear. Modification of the carbohydrates of membrane glycoproteins on intact stimulating or target cells has been observed to inhibit (1, 5, 6), stimulate (7, 8), or have no effect (2, 6, 9) on T cell responses. In all of these cases, it is difficult to conclude whether the observed effects were due to altered MHC antigen oligosaccharides, since modifications could not be limited to the surface glycoprotein of interest, and the extent of modification could not be readily determined.Class I antigens of the murine MHC (H-2) are polymorphic two-chain cell surface glycoproteins consisting of a glycosylated transmembrane heavy chain (Mr 40,000-50,000) noncovalently associated with /32-microglobulin, an invariant, nonglycosylated polypeptide (Mr 1 1,500). Amino acid and DNA sequence data suggest that the extracellular portion of the heavy chain can be divided into three globular domains of ~91 amino acids each (~, c~z, and as). Determinants recognized by T cells appear to reside in the c~l and a2 domains (10), regions of maximal amino acid variability (11). Glycosyl moieties are also present in these regions, N-linked to asparagine residues at positions 86 and 176. A third glycan attachment site in K d, D b, and L d antigens is at residue 256 (11).Purified class I alloantigen in artificial membranes (liposomes) can stimulate a CTL response of comparable magnitude to that obtained using intact stimulating cells when added to in vitro cultures of spleen cells from mice previously primed to the same alloantigen (12). The magnitude of the response to the liposomes provides a direct measure of the effectiveness of antigen recognition

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“…The binding of lymphocytes to target cells has been demonstrated repeatedly, and several methods are available for the study of the binding process [6,[9][10][11][12]. Macrophage and fibroblast monolayers were initially used [9-121.…”

Section: Discussionmentioning

confidence: 99%

Lymphocyte‐Mediated Cytolysis

Berke

1988

Annals of the New York Academy of Sciences

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Modification of in vitro immune lymphocyte-target cell interaction by some biologically active drugs

Cited by 7 publications

References 0 publications

T cell recognition of a tumor-associated glycoprotein and its synthetic carbohydrate epitopes: stimulation of anticancer T cell immunity in vivo

T cell recognition of a tumor-associated glycoprotein and its synthetic carbohydrate epitopes: stimulation of anticancer T cell immunity in vivo

Carbohydrate moieties of major histocompatibility complex class I alloantigens are not required for their recognition by T lymphocytes.

Lymphocyte‐Mediated Cytolysis

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