Modification of glycosylated haemoglobin concentration during artificial endocrine pancreas treatment of diabetics

Bolli, Geremia B.; Cartechini, M. G.; Compagnucci, P.; Santeusanio, Fausto; Massi‐Benedetti, Massimo; Calabrese, Giuseppe; Puxeddu, A; Brunetti, P

doi:10.1007/bf00290488

Cited by 35 publications

(8 citation statements)

References 21 publications

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“…2). The usefulness of HbAla_ r in monitoring long-term control of glycaemia has recently been disputed in view of several reports indicating that glycohaemoglobin levels may fall after correction of hyperglycaemia much more rapidly than expected [18][19][20][21][22][23][24][25][26]. No such rapid changes of HbAI~_ ~ could be observed in the present study.…”

Section: Discussioncontrasting

confidence: 79%

Different behaviour of haemoglobin A1a?c and glycosyl-albumin levels during recovery from diabetic ketoacidosis and non-acidotic coma

Dolhofer¹,

Renner²,

Wieland³

1981

Diabetologia

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Glycosylated haemoglobin (HbA1a-c) and serum albumin (glycosyl-albumin) have been determined in patients with severe diabetic ketoacidosis and non-acidotic coma. Within one week of therapy the level of glycosyl-albumin decreased from 184 mmol 5-hydroxymethylfurfural (HMF)/mol albumin to 152 mmol HMF/mol albumin (p less than 0.01) and was gradually lowered by some 40% during a period of 17 days. In contrast, the level of HbA1a-c remained unchanged. From these observations and findings in a patient with insulinoma, it appears that glycosyl-albumin provides a more acute measure of variation in relative glycaemia than HbA1a-c, and may prove useful as a measure of medium-term diabetes control.

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Section: Discussioncontrasting

confidence: 79%

Different behaviour of haemoglobin A1a?c and glycosyl-albumin levels during recovery from diabetic ketoacidosis and non-acidotic coma

Dolhofer¹,

Renner²,

Wieland³

1981

Diabetologia

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“…Rapid in vivo changes in HbA1 concentration have been noted less frequently. Karamanos [25] and Bolli [26] found significant changes in diabetics within 3 days. Leslie [27] reported a mean decrease of 2.5% HbA1 in 1 week.…”

Section: Discussionmentioning

confidence: 99%

Rapid changes in chromatographically determined haemoglobin A1c induced by short-term changes in glucose concentration

et al. 1980

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Chromatographically determined haemoglobin A1c concentration was measured during short-term (1-24h) changes in glucose concentration in vitro and in vivo. In vitro at 37 degrees C the HbA1c concentration increased with glucose concentration and time both in normal and diabetic erythrocytes. In normal erythrocytes incubated in 20--100 mmol/l glucose, the increases in the HbA1c concentration were maximal after 4--6 h and then stable for the next 18--20 h. During the first hour, increases in the HbA1c concentration were linear with time and on average 0.034% HbA1c x h-1 x mmol/l glucose-1. In erythrocytes, after a rapidly produced increase (2 h), HbA1c decreased to preincubation concentrations during a further incubation of the erythrocytes in a glucose-free medium at 37 degrees C for 4--6 h. The mean rate of linear decrease was 0.017% x h-1 x mmol/l glucose-1. After incubation of erythrocytes in 100 mmol/l glucose for 24 h, 1.3% HbA1c remained stable for 6 h in saline. The rapid increase in HbA1c concentration, as determined by chromatography, was not due to stable HbA1c (ketoamine linked glucose) as no increase was found in the HbA1c concentrations determined by the thiobarbiturate method. In juvenile diabetics controlled by an artificial beta-cell, rapid changes of blood glucose concentration (up to 20 mmol/l) resulted in increases in HbA1c concentration of as much as 1.9% within 12 h (mean 1.1%). Rapid in vivo increases in HbA1c concentration were reversible by normalization of the blood glucose concentration. That rapid changes in HbA1c may occur in daily diabetic life was evidenced by differences in HbA1c concentration between blood samples from out-patient diabetics incubated in saline for 16 hours at 4 degrees C and 37 degrees C (range of differences 0.2--1.4% HbA1c). The differences correlated to the blood glucose concentration at the time of sampling blood for HbA1c determination. Thus, incubation of blood at a low glucose concentration prior to determination of the glycosylated haemoglobin concentration may overcome interference from rapidly produced HbA1c.

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“…The determination of HbA j was carried out in duplicate by a rapid chromatographic method (1 h) [5,6], in thermostatically controlled macrocolumns (18 DC). Before chromatography, all haemolysates were gel-filtered with developer No.6 [7] in macrocolumns (internal diameter 1.5 cm), filled with Sephadex G-25 (10 cm high) at 4 DC, thus ensuring that haemolysates had the same pH and ionic strength as the eluent buffer.…”

Section: Chromatographymentioning

confidence: 99%

Analysis of short-term changes in reversibly and irreversibly glycosylated haemoglobin AI: Relevance to diabetes mellitus

et al. 1981

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We have determined the stable (irreversibly glycosylated) fraction of haemoglobin AI (HbAI) on Bio-Rex 70 after incubation of red blood cells in 0.9 % saline solution for 6 h at 37 degrees C. The total (reversibly + irreversibly glycosylated fractions) HbAI was determined before each incubation. Labile (reversibly glycosylated) HbAI represented the difference between total and stable HbAI fractions. Total and stable HbAI fractions were determined during insulin- or meal-induced blood glucose fluctuations in 24 insulin-dependent diabetics and in seven subjects with impaired glucose tolerance. In the diabetics, the maximal fluctuation of total HbAI was 1.47% over 2-12 h, while the simultaneous plasma glucose variation was 21.5 mmol/l. The stable HbAI fraction did not change significantly. In diabetics the differences between the maximal and minimal values of plasma glucose and total HbAI were significantly correlated. Plasma glucose correlated with simultaneously determined total and labile HbAI fractions, but not with stable HbAI. In subjects with impaired glucose tolerance, similar changes in total but not in stable HbAI were observed during an oral glucose tolerance test. We conclude that, although rapid changes in chromatographically determined HbAI are relatively small, the determination of stable HbAI should be performed to circumvent this problem and to ensure a more accurate index of blood glucose control.

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Modification of glycosylated haemoglobin concentration during artificial endocrine pancreas treatment of diabetics

Cited by 35 publications

References 21 publications

Different behaviour of haemoglobin A1a?c and glycosyl-albumin levels during recovery from diabetic ketoacidosis and non-acidotic coma

Different behaviour of haemoglobin A1a?c and glycosyl-albumin levels during recovery from diabetic ketoacidosis and non-acidotic coma

Rapid changes in chromatographically determined haemoglobin A1c induced by short-term changes in glucose concentration

Analysis of short-term changes in reversibly and irreversibly glycosylated haemoglobin AI: Relevance to diabetes mellitus

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