Modification of glass channel walls for separation of biological particles by gravitational field-flow fractionation

Plocek, Jiřı́; Konečný, Přemysl; Chmelı́k, Josef

doi:10.1016/s0378-4347(94)80106-1

Cited by 20 publications

(5 citation statements)

References 14 publications

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“…As has been widely described, different types of FFF techniques can be distinguished according to the nature of the applied external field; one of these techniques is gravitational field‐flow fractionation (GrFFF), a sedimentation FFF subtechnique that employs the Earth's gravitational field applied perpendicularly to the channel. Over the years, GrFFF has been shown to be suitable for the characterization of various micrometer‐size particles of different origin, particularly for biological samples as applications have demonstrated (8–13). This technique is very advantageous for cell sorting and posterior characterization as a result of its simplicity, reduced risk of sample degradation, and low cost.…”

Section: Introductionmentioning

confidence: 99%

Determination of Viable Yeast Cells by Gravitational Field‐Flow Fractionation with Fluorescence Detection

Sanz

Galcerán

Puignou

2004

Biotechnology Progress

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Vinification processing is largely related to yeast performance and depends on the initial cell viability. To optimize the quality of wine fermentation, control of the yeast quality is mandatory. The present paper describes a new method using gravitational field flow fractionation (GrFFF) with fluorescence detection for the determination of yeast cell viability before the fermentation process. A GrFFF calibration procedure was developed using commercial yeast to prepare standards of viable cells and propidium iodide (PI) as fluorescent probe for nonviable cells. The suitability of the new method was tested with several commercial yeast strains with a g/L content ranging from 1 to 3. The validation of the method was performed by comparing GrFFF viability values with those obtained using Coulter counter and flow cytometry techniques.

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Section: Introductionmentioning

confidence: 99%

Determination of Viable Yeast Cells by Gravitational Field‐Flow Fractionation with Fluorescence Detection

Sanz

Galcerán

Puignou

2004

Biotechnology Progress

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“…The gravitational force and the hydrodynamic lift-forces are the most apparent that affect the particles in GFFF. However, previous experiments showed that other forces also can influence the separation process, namely surface (colloidal) forces (the electrostatic and van der Waals forces), although most previous papers on FFF regarded them as relatively small perturbations (SdFFF in centrifuge) [28-301. In the case of GFFF, Cardot and colleagues [13, 19, [32] attempted to suppress the electrostatic forces by coating the accumulation wall by a nonionic layer (silicon oils, polystyrene lacquer) or by making the wall from polycarbonate [HI. Pazourek and colleagues [lo,161 presented factograms of a mixture of polystyrene beads obtained in aqueous solutions of different surfactants as the carrier liquids.…”

Section: Introductionmentioning

confidence: 99%

Effect of flowrate and ionic strength on retention of nonporous micron-sized silica gel particles in gravitational field-flow fractionation

2005

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Abstract. Retention of nonporous micron-sized silica gel particles in gravitational field-flow fractionation (GFFF) was investigated with respect to the activity of the hydrodynamic lift-forces and surface forces. The hydrodynamic lift-forces act near the channel wall in the same direction as repulsive electrostatic forces against the gravity so that the particles are focused. Although a complete quantitative description of the hydrodynamic lift-forces, which would enable prediction of the retention time, is not yet available, the experiments show that the magnitude of both forces is comparable with the particle effective weight and that the forces can be used for efficient separation of micron-sized particles. Higher ionic strengths of the carrier liquid attained by addition of NaCl decrease the repulsive electrostatic force and dramatically influence retention of the particles, yet to be reported in GFFF. o 1996 John Wiley & Sons, Inc.

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“…Field-flow fractionation (FFF) is a separation technique for macromolecules, and can be useful in various fields including biotechnology. [4][5][6] FFF is also well suited to analytical-scale separation and characterization of nano to micron-sized particles. 7,8 FFF has been successfully applied to characterization and separation of colloids with biological interest such as yeast.…”

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confidence: 99%

Monitoring of Mixed Culture of Saccharomyces cerevisiae and Acetobacter aceti Using Gravitation Field-flow Fractionation and Gas Chromatography

Kim¹,

Kim²,

Kim³

et al. 2013

Bulletin of the Korean Chemical Society

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Modification of glass channel walls for separation of biological particles by gravitational field-flow fractionation

Cited by 20 publications

References 14 publications

Determination of Viable Yeast Cells by Gravitational Field‐Flow Fractionation with Fluorescence Detection

Determination of Viable Yeast Cells by Gravitational Field‐Flow Fractionation with Fluorescence Detection

Effect of flowrate and ionic strength on retention of nonporous micron-sized silica gel particles in gravitational field-flow fractionation

Monitoring of Mixed Culture of Saccharomyces cerevisiae and Acetobacter aceti Using Gravitation Field-flow Fractionation and Gas Chromatography

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