Modification of expression of stem cell factor by various cytokines

Abstract

The local production of stem cell factor (SCF) may be an important mechanism for regulating proliferation, differentiation, and migration of various cells bearing c-kit receptors, and might be susceptible to the cytokines that serve in inflammation and tissue repair. We have demonstrated that in three murine cell lines, Balb/3T3A31, MC3T3-E1, and C3H-2K, which constitutively produced SCF with different quantity, the SCF mRNA expression was greatly enhanced in response to basic fibroblast growth factor (bFGF) or transforming growth factor beta1 (TGF-beta1). The study was carried out by in situ hybridization utilizing nonradioactive oligonucleotide probes and quantitative image analysis. Leukemia inhibitory factor (LIF) or interleukin-4 (IL-4) moderately increased SCF mRNA in all cell lines, but IL-3 did not. The dot-blot enzyme-linked immunosorbent assay (ELISA) further confirmed that SCF protein production in these cell lines and bone marrow stromal cells was markedly enhanced by TGF-beta1, although TGF-beta1 suppressed the proliferation of all these cells. bFGF also enhanced the SCF production in these cell lines, but did not in bone marrow stromal cells, suggesting a difference in their susceptibility to the cytokine. Our results suggest that TGF-beta1 and bFGF potentially modulate the biological function of cells bearing c-kit receptors through the modulation of SCF production in fibroblasts.

c-Jun Induces Mammary Epithelial Cellular Invasion and Breast Cancer Stem Cell Expansion

“…Primers for all the genes were either designed using Primer Express 5.1 (Applied Biosystems Inc., Foster City, CA) (Table 1) or as described in Ref. 41.…”

Proteomic changes associated with diabetes in the BB-DP rat

“…3-Oxo-5-␤-steroid 4-dehydrogenase is necessary in bile acid synthesis (31) and as such may have a role in the hypercholesterolemia that accompanies diabetes. D-Dopachrome tautomerase was downregulated in diabetes and is an inflammatory mediator important in T cell and macrophage migration inhibition (47,63) with no established role in diabetes beyond that ketones are effective inhibitors of this enzyme (19). Liver calcium/calmodulin-dependent 3Ј,5Ј-cyclic nucleotide phosphodiesterase 1C (CDCDP) was significantly downregulated in diabetes, suggesting a modification of the calcium signaling/handling pathways in diabetic liver as described for the heart.…”

Somatic Excision Demonstrates that c-Jun Induces Cellular Migration and Invasion through Induction of Stem Cell Factor

“…RNA quantitation was done in an Agilent 2100 Bioanalyzer (Palo Alto, CA), and equal quantities were used for the reverse transcription reactions. Primers for all the genes including housekeeping control gene transcripts were either designed using Primer Express 5.1 (Applied Biosystems Inc., Foster City, CA) (Table 1) or referenced from Sugimoto et al (54). Chromatin immunoprecipitation (ChIP) analysis was performed according to a standard protocol provided by Upstate Biotechnology, Inc., with minor modifications (14,22).…”