Modification of electrical characteristics of bilayer lipid membranes by intramitochondrial thyroid hormone receptor

Kozhomkulov, E.T.; Normatov, K; Azimova, Sh. S.; Marzoev, A. I.

doi:10.1007/bf01149498

Cited by 1 publication

(1 citation statement)

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“…One study of the effects of membrane proteins on electroporation claimed a twofold increase in the electroporation threshold after the insertion of liver mitochondrial proteins that increased membrane conductance (Kozhomkulov et al, 1984). Other researchers have speculated that transmembrane proteins or peptides could pack along the edges of pores and deter their resealing, thus making membrane resealing more difficult (Chernomordik et al, 1987).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Gramicidin on Electroporation of Lipid Bilayers

Troiano

Stebe

Raphael

et al. 1999

Biophysical Journal

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The effects of the channel-forming peptide gramicidin D (gD) on the conductance and electroporation thresholds of planar bilayer lipid membranes, made of the synthetic lipid 1-palmitoyl 2-oleoyl phosphatidylcholine (POPC), was studied. High-amplitude ( approximately 200-900 mV) rectangular voltage pulses of 15 ms duration were used to perturb the bilayers and monitor the transmembrane conductance. Electroporation voltage thresholds were found, and conductance was recorded before and after electroporation. Gramicidin was added to the system in peptide/lipid ratios of 1:10, 000, 1:500, and 1:15. The addition of gD in a ratio of 1:10,000 had no effect on electroporation, but ratios of 1:500 and 1:15 significantly increased the thresholds by 16% (p < 0.0001) and 40% (p < 0.0001), respectively. Membrane conductance before electroporation was measurable only after the addition of gD and increased monotonically as the peptide/lipid ratio increased. The effect of gD on the membrane area expansivity modulus (K) was tested using giant unilamellar vesicles (GUVs). When gD was incorporated into the vesicles in a 1:15 ratio, K increased by 110%, consistent with the increase in thresholds predicted by an electromechanical model. These findings suggest that the presence of membrane proteins may affect the electroporation of lipid bilayers by changing their mechanical properties.

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Section: Introductionmentioning

confidence: 99%