Modification of Collagen for Biomedical Applications: A Review of Physical and Chemical Methods

Yu, Xiaoyue; Tang, Cuie; Xiong, Shanbai; Yuan, Qijuan; Gu, Zhipeng; Li, Zhen; Hu, Yang

doi:10.2174/1385272820666151102213025

Cited by 43 publications

(17 citation statements)

References 140 publications

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“…Gelatin, being low in calories, is widely used in food, photographic, cosmetic, biomedical, pharmaceutical, and neutraceutical industries (Al‐Kahtani et al, ; Kumar, Chandra, Elavarasan, & Shamasundar, ; Sha et al, ; Yu et al, ). It has been reported that the global demand for gelatin is expected to reach 450.7 kt in 2018 (Huang et al, ), with around 98.5% is extracted from mammalian sources including pig skins, bovine hides, and beef bones, etc.…”

Section: Introductionmentioning

confidence: 99%

Comparison of gelling properties and flow behaviors of microbial transglutaminase (MTGase) and pectin modified fish gelatin

Huang

Zhao

Fang

et al. 2019

Journal of Texture Studies

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The gelling and structural properties of microbial transglutaminase (MTGase) and pectin modified fish gelatin were compared to investigate their performances on altering fish gelatin properties. Our results showed that within a certain concentration, both MTGase and pectin had positive effects on the gelation point, melting point, gel strength, textural, and swelling properties of fish gelatin. Particularly, low pectin content (0.5%, w/v) could give fish gelatin gels the highest values of gel strength, melting temperature, and hardness. Meantime, flow behavior results showed that both MTGase and pectin could increase fish gelatin viscosity without changing its fluid characteristic, but the latter gave fish gelatin higher viscosity. Both MTGase and pectin could increase the lightness of fish gelatin gels but decreases its transparency. More importantly, fluorescence and UV absorbance spectra, particle size distribution, and confocal microscopy results indicated that MTGase and pectin could change the structure of fish gelatin with the formation of large aggregates. Compared with MTGae modified fish gelatin, pectin could endow fish gelatin had similar gel strength, thermal and textural properties to pig skin gelatin.

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Section: Introductionmentioning

confidence: 99%

Comparison of gelling properties and flow behaviors of microbial transglutaminase (MTGase) and pectin modified fish gelatin

Huang

Zhao

Fang

et al. 2019

Journal of Texture Studies

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“…The uterine horn, like the human Fallopian tube, is a moist tubal environment with dynamic fluid secretion and absorption and a virtual and corrugated lumen, requiring a conformable implant. An EDS-NHS crosslinked, porous collagen scaffold unlocked the functionalities of fluid absorption and resiliency to fill the uterine space without compaction of the porosity; these properties are a result of the covalent bonds formed through chemical crosslinking [10]. The conserved porosity was visible both under confocal microscopy (Figure 1A) and in situ (Figure 3B), validating that our structure is robust for this application.…”

Section: Discussionmentioning

confidence: 55%

Design, Manufacture, and In vivo Testing of a Tissue Scaffold for Permanent Female Sterilization by Tubal Occlusion

et al. 2018

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Current FDA-approved permanent female sterilization procedures are invasive and/or require the implantation of non-biodegradable materials. These techniques pose risks and complications, such as device migration, fracture, and tubal perforation. We propose a safe, non-invasive biodegradable tissue scaffold to effectively occlude the Fallopian tubes within 30 days of implantation. Specifically, the Fallopian tubes are mechanically de-epithelialized, and a tissue scaffold is placed into each tube. It is anticipated that this procedure can be performed in less than 30 minutes by an experienced obstetrics and gynaecology practitioner. Advantages of this method include the use of a fully bio-resorbable polymer, low costs, lower risks, and the lack of general anaesthesia. The scaffold devices are freeze-cast allowing for the custom-design of structural, mechanical, and chemical cues through material composition, processing parameters, and functionalization. The performance of the biomaterial and de-epithelialization procedure was tested in an in vivo rat uterine horn model. The scaffold response and tissue-biomaterial interactions were characterized microscopically post-implantation. Overall, the study resulted in the successful fabrication of resilient, easy-to-handle devices with an anisotropic scaffold architecture that encouraged rapid bio-integration through notable angiogenesis, cell infiltration, and native collagen deposition. Successful tubal occlusion was demonstrated at 30 days, revealing the great promise of a sterilization biomaterial.

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“…Collagen, as the most abundant protein in mammals, has been extensively researched and applied in the fields of medicine, tissue engineering, cosmetics, and so on, owing to its advantages such as good biocompatibility, low antigenicity, and controlled biodegradability . Therefore, collagen has been widely accepted as a quite promising biomaterial, however, some defects, particularly, the low mechanical strength, poor thermal stability, and vulnerability to enzymatic degradation still cannot be overlooked, which significantly restricted the applications of collagen . Accordingly, many chemical modifiers such as glutaraldehyde (GTA) , carbodiimide , acylazide , genipin , plant tannins , polyepoxy compound , and dialdehyde compound derived from natural biomass have been employed on collagen in order to obtain ideal physicochemical performance and thus meet the requirements for in vivo and in vitro applications.…”

Section: Introductionmentioning

confidence: 99%

“…Of the various modifiers, carbodiimide could form a typical “zero‐length” cross‐linking between collagen molecules , and thus perfectly avoiding any extra cytotoxicity into the modified collagen. In addition, N‐hydroxysuccinimide (NHS) could assist the O‐acylisourea group in converting into the more stable NHS‐activated carboxylic acid group , enhancing the carbodiimide cross‐linking efficiency for collagen . It should be pointed out that, however, the carboxylic and amine groups were not easy to be cross‐linked each other by carbodiimide for their distance.…”

Section: Introductionmentioning

confidence: 99%

Insight into the rheological behaviors of a polyanionic collagen fabricated with poly(γ‐glutamic acid)‐NHS ester

Ding

Yang

Lan

et al. 2019

Biotech and App Biochem

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The rheological behaviors of a polyanionic collagen, fabricated using poly(γ -glutamic acid)-N-hydroxysuccinimide (γ -PGA-NHS) as a novel modifier, were investigated in this study. It was found that both of the native and modified collagen solutions were pseudoplastic fluids, as shown from the steady-shear tests. While the storage modulus and loss modulus of collagen increased with increasing the amount of γ -PGA-NHS, or with increasing the degree of esterification of γ -PGA-NHS; meanwhile, the dynamic denaturation temperature determined by dynamic temperature sweep was also increased, indicating an improved thermal stability of collagen solution modified by γ -PGA-NHS. The creeprecovery measurements showed that the resistance to deformation was enhanced for modified collagen, probably due to the cross-linking occurred between the ε-amino groups of collagen molecules and α-COOH groups of γ -PGA-NHS, as well as the electrostatic interaction and hydrogen-bond interactions between the two molecules. Furthermore, the aggregation of collagen fibers was promoted due to these interactions between collagen and γ -PGA-NHS as observed by atomic force morphology. In addition, the modified collagen exhibited good cytocompatibility as demonstrated by cell growth culturing. The obtained information was expected to give valuable clues to the design and fabrication of controlled stable collagen-based products for applications in various biomedical fields.

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Modification of Collagen for Biomedical Applications: A Review of Physical and Chemical Methods

Cited by 43 publications

References 140 publications

Comparison of gelling properties and flow behaviors of microbial transglutaminase (MTGase) and pectin modified fish gelatin

Comparison of gelling properties and flow behaviors of microbial transglutaminase (MTGase) and pectin modified fish gelatin

Design, Manufacture, and In vivo Testing of a Tissue Scaffold for Permanent Female Sterilization by Tubal Occlusion

Insight into the rheological behaviors of a polyanionic collagen fabricated with poly(γ‐glutamic acid)‐NHS ester

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