Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to metal stress

Machado, Manuela D.; Soares, Eduardo V.

doi:10.1016/j.aquatox.2013.11.017

Cited by 55 publications

(56 citation statements)

References 30 publications

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“…This is in line with other studies that demonstrated that in poor-quality environments populations invest in maintaining cell condition (38,39). In C. reinhardtii, cell size is related to population growth through its effect on cell division, as a critical size must be reached before cells divide (40)(41)(42). This trend of phenotypic reversion during adaptive evolution has also been seen in high-CO 2 environments (43), indicating that some phenotypic reversion may be a common outcome of evolution (44).…”

Section: Discussionsupporting

confidence: 73%

Evolutionary consequences of multidriver environmental change in an aquatic primary producer

Brennan

Colegrave

Collins

2017

Proc. Natl. Acad. Sci. U.S.A.

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Climate change is altering aquatic environments in a complex way, and simultaneous shifts in many properties will drive evolutionary responses in primary producers at the base of both freshwater and marine ecosystems. So far, evolutionary studies have shown how changes in environmental drivers, either alone or in pairs, affect the evolution of growth and other traits in primary producers. Here, we evolve a primary producer in 96 unique environments with different combinations of between one and eight environmental drivers to understand how evolutionary responses to environmental change depend on the identity and number of drivers. Even in multidriver environments, only a few dominant drivers explain most of the evolutionary changes in population growth rates. Most populations converge on the same growth rate by the end of the evolution experiment. However, populations adapt more when these dominant drivers occur in the presence of other drivers. This is due to an increase in the intensity of selection in environments with more drivers, which are more likely to include dominant drivers. Concurrently, many of the trait changes that occur during the initial short-term response to both single and multidriver environmental change revert after about 450 generations of evolution. In future aquatic environments, populations will encounter differing combinations of drivers and intensities of selection, which will alter the adaptive potential of primary producers. Accurately gauging the intensity of selection on key primary producers will help in predicting population size and trait evolution at the base of aquatic food webs.

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Section: Discussionsupporting

confidence: 73%

Evolutionary consequences of multidriver environmental change in an aquatic primary producer

Brennan

Colegrave

Collins

2017

Proc. Natl. Acad. Sci. U.S.A.

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“…The lower and upper boundaries selected correspond to individual algae cell size distribution. These values are in agreement with the data recently reported in the literature, which described an average size (length×width) of 9.1× 3.2 μm for P. subcapitata (Machado and Soares 2014). It is important to note that a histogram of "cell size vs. number of cells" is always displayed on the equipment screen.…”

Section: Discussionsupporting

confidence: 80%

“…However, the slow growth (doubling time of~12 h) of P. subcapitata cells (Machado and Soares 2014) makes it difficult to use this method, as it takes 1-2 weeks to visibly observe colonies. In this context, the staining exclusion assay emerges as a good alternative.…”

Section: Discussionmentioning

confidence: 99%

Quantification and viability analyses of Pseudokirchneriella subcapitata algal cells using image-based cytometry

Machado

Soares

2014

J Appl Phycol

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This work aims to evaluate the feasibility of using image-based cytometry (IBC) in the analysis of algal cell quantification and viability, using Pseudokirchneriella subcapitata as a cell model. Cell concentration was determined by IBC to be in a linear range between 1×10 5 and 8×10 6 cells mL −1. Algal viability was defined on the basis that the intact membrane of viable cells excludes the SYTOX Green (SG) probe. The disruption of membrane integrity represents irreversible damage and consequently results in cell death. Using IBC, we were able to successfully discriminate between live (SG-negative cells) and dead algal cells (heattreated at 65°C for 60 min; SG-positive cells). The observed viability of algal populations containing different proportions of killed cells was well correlated (R 2 =0.994) with the theoretical viability. The validation of the use of this technology was carried out by exposing algal cells of P. subcapitata to a copper stress test for 96 h. IBC allowed us to follow the evolution of cell concentration and the viability of copperexposed algal populations. This technology overcomes several main drawbacks usually associated with microscopy counting, such as labour-intensive experiments, tedious work and lack of the representativeness of the cell counting. In conclusion, IBC allowed a fast and automated determination of the total number of algal cells and allowed us to analyse viability. This technology can provide a useful tool for a wide variety of fields that utilise microalgae, such as the aquatic toxicology and biotechnology fields.

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“…Three nominal concentrations of each metal [Cd(II), Cr(VI), Cu(II) and Zn(II)] were used, as previously defined (Machado and Soares 2014)…”

Section: Methodsmentioning

confidence: 99%

Use of a fluorescence-based approach to assess short-term responses of the alga Pseudokirchneriella subcapitata to metal stress

Machado

Soares

2014

J Appl Phycol

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This work explores the use of fluorescent probes to evaluate the responses of the green alga Pseudokirchneriella subcapitata to the action of three nominal concentrations of Cd(II), Cr(VI), Cu(II) and Zn(II) for a short time (6 h). The toxic effect of the metals on algal cells was monitored using the fluorochromes SYTOX Green (SG, membrane integrity), fluorescein diacetate (FDA, esterase activity) and rhodamine 123 (Rh123, mitochondrial membrane potential). The impact of metals on chlorophyll a (Chl a) autofluorescence was also evaluated. Esterase activity was the most sensitive parameter. At the concentrations studied, all metals induced the loss of esterase activity. SG could be used to effectively detect the loss of membrane integrity in algal cells exposed to 0.32 or 1.3 μmol L −1 Cu(II). Rh123 revealed a decrease in the mitochondrial membrane potential of algal cells exposed to 0.32 and 1.3 μmol L −1 Cu(II), indicating that mitochondrial activity was compromised. Chl a autofluorescence was also affected by the presence of Cr(VI) and Cu(II), suggesting perturbation of photosynthesis. In conclusion, the fluorescence-based approach was useful for detecting the disturbance of specific cellular characteristics. Fluorescent probes are a useful diagnostic tool for the assessment of the impact of toxicants on specific targets of P. subcapitata algal cells.

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Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to metal stress

Cited by 55 publications

References 30 publications

Evolutionary consequences of multidriver environmental change in an aquatic primary producer

Evolutionary consequences of multidriver environmental change in an aquatic primary producer

Quantification and viability analyses of Pseudokirchneriella subcapitata algal cells using image-based cytometry

Use of a fluorescence-based approach to assess short-term responses of the alga Pseudokirchneriella subcapitata to metal stress

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