Modification of a transplantable colon tumor and immune responses in mice fed different sources of protein, fat and carbohydrate

Nutter, Robert L.; Gridley, Daila S.; Andres, Melba L.; Aprecio, Raydolfo M.; Slater, James M.

doi:10.1016/0304-3835(83)90117-9

Cited by 15 publications

(6 citation statements)

References 19 publications

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“…In diet and cancer studies with three different mouse tumor systems (18)(19)(20)(21), we observed that in addition to level of dietary fat, the source of dietary protein also affected tumor development. The milk-or casein-fed groups had less tumor growth and higher mitogeninduced lymphoproliferative ability compared with other proteins that were fed, including beef.…”

Section: Introductionmentioning

confidence: 89%

“…51 cells [a DMH-induced colon tumor cell line derived in our laboratories (20)] labeled with [ 125 I]iododeoxyuridine (IdUrd) were used as target cells (19,24,25). The assays were performed in triplicate in microtiter plates at effector spleen cell-to-target cell ratios of 500:1.…”

Section: Methodsmentioning

confidence: 99%

“…In mouse tumor systems used in previous diet studies in our laboratories (18)(19)(20), RSW values were increased with tumor growth. In this study, the Diet 1 group of DMH-injected mice had the highest RSW and also the greatest tumor burden.…”

mentioning

confidence: 87%

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Effects of dietary fat and protein on DMH‐induced tumor development and immune responses

Nutter

Kettering

Aprecio

et al. 1990

Nutrition and Cancer

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Although in three different mouse tumor systems with corn oil as dietary fat we previously found that milk protein decreased tumor development compared with beef, the results were reversed in 1,2-dimethylhydrazine (DMH)-injected mice. The purpose of this study was to determine if the latter result was due to the protein source. BALB/c mice (n = 280) were divided into five diet groups and injected 10 times at weekly intervals with DMH (20 mg/kg wt) or saline. Four diets contained 11% protein (casein, milk, or beef) and 5% fat (corn oil or beef tallow), and the AIN-76A diet was used as a control diet. The source of fat was a significant modulator of tumor development. Corn oil markedly increased total tumor volume and the number of tumors per mouse compared with beef tallow. Its tumor-enhancing effects were evident when it was combined with milk but not with casein. In addition, significantly lower lymphoproliferation and T-cell cytotoxicity against colon tumor cell targets was associated with corn oil consumption, whereas nonfat milk as the protein source was related to normal oxidative burst capacity of phagocytes. These results demonstrate that the source of dietary fat, in addition to the protein source, has a profound effect on both tumor development and immune responsiveness in this animal tumor system.

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Section: Introductionmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

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Effects of dietary fat and protein on DMH‐induced tumor development and immune responses

Nutter

Kettering

Aprecio

et al. 1990

Nutrition and Cancer

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“…In 1983, Reddy et al 22 indicated that yogurt reduced the number of tumor cells by a factor 0.2-0.28 in mice which were inoculated with Ehrlich ascites tumor cells. Furthermore, in the study of Nutter et al 23 the mice which were under a milk protein formula diet, displayed an inhibition of tumor volume by a factor of 0.2-0.7. The study of Tsuru et al 24 indicated that various types of cheese and yogurt suppressed the growth of several tumors in mice, and tumor size was reduced by a factor of 0.17-0.70 depending on the type.…”

Section: Food Proteinsmentioning

confidence: 96%

Use of Proteins as Biomarkers and Their Role in Carcinogenesis

Zarogoulidis¹,

Tsakiridis²,

Karapantzou³

et al. 2015

J. Cancer

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Summary: Improved diagnostic methods and medical therapies are necessary for early detection and treatment and an improved prognosis. It is thus vital to both examine and evaluate the role of the various existing proteins as biomarkers in carcinogenesis and to assess the contribution of these proteins in anti-cancer activity, for consideration in therapeutic strategies. It is essential to both examine and evaluate the role of the various existing proteins as biomarkers in carcinogenesis and to assess the contribution of these proteins in anti-cancer activity, for consideration in therapeutic strategies. The purpose of this review is twofold. Firstly, it is to evaluate recent data about which proteins can be utilized as biomarkers in carcinogenesis. The proteins reviewed include: CPTP, IL-6, CCN, and S100. Secondly, it is to evaluate the contribution of dietary proteins in cancer activity. Specifically, how whey protein, soy proteins and lectin, a phytochemical could be useful in cancer prevention and treatment.Recent Findings: Whey protein, present in dairy products, is an excellent source of the sulphur amino acid cysteine, the rate limiting substrate in glutathione synthesis. Notably, this protein survives digestion and has been shown to have anti-carcinogenic properties in animal studies. Lectins are phytochemicals present in plant foods, and have active components which alters cancer initiation, promotion and progression. Lectins have been characterized as a useful tool in biochemistry, cell biology, immunology and in diagnostic and therapeutic purposes in cancer research. Soy proteins contain various compounds, including isoflavones, protease inhibitors and protein kinase inhibitors, which have been proven effective in tumor growth inhibition. They have therefore, been greatly emphasized in cancer prevention and treatment. It has been proved that soy food consumption was associated with decreased risk of death and recurrence of breast cancer. CPTP is a recently discovered protein whose main role is to transport C1P, a pro-inflammatory molecule. The discovery of CPTP may shine a light on the mechanism of inflammatory diseases, and hopefully offer a potential target for therapeutic purposes in cancer research. Interleukin-6 is a multifunctional cytokine that affects the activity of cancer cells. It is involved in tumor growth, and elevated levels is associated with an increased risk of cancer. S100B is a well-established biomarker for malignant melanoma, and useful in assessing tumor load, stage and prognosis for patients with this disease. Other members of this family of proteins include S100A4, which has been associated with several malignancies and S100A2, which has been found to be decreased in some cancers. CCN are a group of regulatory proteins, located in the extracellular matrix (maricellular). They are involved in cellular adhesion, mitogenesis, chemotaxis, cell survival, and wound healing. CCN proteins are also able to modulate the signals of several proteins, which may also influence skeletal develo...

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“…Our laboratory has been studying two BALB/c mouse tumor systems; one in which tumors are produced by injection of chemically-transformed cells (#51), and the other in which the tumors are produced as a result of injection of virally-transformed cells (H238) (18,(38)(39)(40). With both systems, tumor-bearing mice showed splenomegaly.…”

Section: Introductionmentioning

confidence: 99%

Effects of the removal of adherent and phagocytic cells on the spleen cell lymphoproliferative response of tumor-bearing mice

Hyde

Stagg

McEachron

et al. 1985

Cancer Immunol Immunother

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Cell-mediated immunity was investigated in two BALB/c mouse tumor systems using the lymphoblastogenesis test with phytohemagglutinin as the mitogen. This lymphoproliferative response was quantitated using the Stimulation Index (SI). There was little evidence for suppressor cell activity in cell mixing experiments in which spleen cells from #51 cell-injected mice were mixed with spleen cells from normal mice. Following macrophage removal by Sephadex G-10 columns and carbonyl iron ingestion, there were no significant changes in the SI values for spleen cells from the #51 cell-injected mice. In contrast, spleen cells from mice injected with H238 cells, a herpes virus-transformed cell line, had a significantly lower SI value than that of normal mice. Suppressor cell activity was demonstrated in cell mixing experiments in which spleen cells from H238 cell-injected mice were mixed with normal spleen cells. Removal of adherent cells from spleen cells from H238 cell-injected mice by Sephadex G-10 columns restored the SI value to that of normal mice. An increased SI value was also seen after removal of phagocytic cells by carbonyl iron. These results suggested that cells with the functional properties of macrophages played an important part in the immunosuppression observed in the H238 tumor system. Comparison of the two macrophage depletion methods suggested that another cell population was also involved in the suppressive effect. Results of immunofluorescent techniques with anti-Lyt-1 and anti-Lyt-2 monoclonal antibodies show these cells to be Ly 1-, Ly 2,3+ phenotypes of T-lymphocytes.

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Modification of a transplantable colon tumor and immune responses in mice fed different sources of protein, fat and carbohydrate

Cited by 15 publications

References 19 publications

Effects of dietary fat and protein on DMH‐induced tumor development and immune responses

Effects of dietary fat and protein on DMH‐induced tumor development and immune responses

Use of Proteins as Biomarkers and Their Role in Carcinogenesis

Effects of the removal of adherent and phagocytic cells on the spleen cell lymphoproliferative response of tumor-bearing mice

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