Modification in oxidative processes in muscle tissues exposed to laser- and light-emitting diode radiation

Abstract

Exposure of living tissues to high-intensity red or near-infrared light can produce the oxidative stress effects both in the target zone and adjacent ones. The protein oxidative modification (POM) products can be used as reliable and early markers of oxidative stress. The contents of modified proteins in the investigated specimens can be evaluated by the 2,4-dinitrophenylhydrazine assay (the DNPH assay). Low-intensity red light is able to decrease the activity of oxidative processes and the DNPH assay data about the POM products in the biological tissues could show both an oxidative stress level and an efficiency of physical agent protection against the oxidative processes. Two control groups of white rats were irradiated by laser light, the first control group by red light and the second one by near-infrared radiation (NIR).Two experimental groups were consequently treated with laser and red low-level light-emitting diode radiation (LED). One of them was exposed to red laser light + LED and the other to NIR + LED. The fifth group was intact. Each group included ten animals. The effect of laser light was studied by methods of protein oxidative modifications. We measured levels of both induced and spontaneous POM products by the DNPH assay. The dramatic increase in levels of POM products in the control group samples when compared with the intact group data as well as the sharp decrease in the POM products in the experimental groups treated with LED low-level light were statistically significant (p ≤ 0.05). Exposure of skeletal muscles to high-intensity red and near-infrared laser light causes oxidative stress that continues not less than 3 days. The method of measurement of POM product contents by the DNPH assay is a reliable test of an oxidative process rate. Red low-intensity LED radiation can provide rehabilitation of skeletal muscle tissues treated with high-intensity laser light.

Normal and pathological ultrastructure of mucous membrane epithelium of the tongue of white rats

“…Furthermore, the number of patients seeking medical help for this problem is steadily increasing. Pathology is known to have a persistent, long-term course, in some cases leading to personal disorganisation of patients [1][2][3][4][5][6][7]. At the same time, therapy for this group of patients has had little success to date.…”

“…One of the the directions of complex treatment of the "glowing mouth" syndrome is physiotherapeutic treatment methods, in particular non-invasive neuromodulation, that is, the regional-integrative influence of red light of broad range [4] (transcranially, in the cervical collar zone and in the area of painful areas of the tongue and mucous membrane of the mouth). However, use of this method in practice is restrained due to lack of clear idea of the character of structural and functional features of epithelium of oral mucosa and tongue as a result of noninvasive neuromodulation.…”

Reabilitação Sensório-Motora Associada À Fotobiomodulação Transcraniana Em Pacientes Com Lesão Do Sistema Nervoso Central

“…A aplicabilidade da Terapia Laser de Baixa Intensidade (TLBI) ou Fotobiomodulação (FBM), como é conhecida atualmente, tem se mostrado efetiva dentro do processo terapêutico para objetivos que variam desde cicatrização de feridas, alívio de dor crônica e processos inflamatórios, diminuição da fadiga, diminuição de marcadores bioquímicos pós exercícios à melhora do desempenho muscular de indivíduos sadios e neurológicos (LEAL JUNIOR et al, 2010;DE MARCHI et al, 2011;ALMEIDA et al, 2011;CHAIEB et al, 2015;REIS et al, 2015;NAMPO et al, 2016;MONICH;BAVRINA;MALINOVSKAYA, 2018;NEVES et al, 2020).…”