Modification-free amperometric biosensor for the detection of wild-type p53 protein based on the in situ formation of silver nanoparticle networks for signal amplification

Hou, Lijun; Huang, Yaliang; Hou, Weilin; Yan, Yurou; Liu, Jinlin; Xia, Ning

doi:10.1016/j.ijbiomac.2020.04.271

Cited by 31 publications

(12 citation statements)

References 60 publications

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“…Application of ECBs encompasses a wide variety of research area from small biomolecules (dopamine, glucose, xanthine, etc.) to cancer biomarkers and other large biological systems [3,[25][26][27][28][29][30][31][32][33][34][35][36][37]42,44]. These biosensors vary from each other based on the use of biorecognition components (BRC) and EAT for the detection process [1,4].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…Among many, gold (AuNPs), silver (AgNPs), and platinum NPs (PtNPs) are some of the most commonly utilized in ECBs [21][22][23][24][25][26][27][28]. In fabricating biosensors, these MNPs often provide the anchoring site for biorecognition components such as antibodies, enzymes, single stranded RNA (ssRNA) and DNA (ssDNA), aptamers, and affibodies [21][22][23][24]27,[29][30][31]. The effectiveness and stability of these biochemical interactions are largely dependent on the physicochemical properties of the MNPs [1,20,32].…”

Section: Introductionmentioning

confidence: 99%

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Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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With the rise in public health awareness, research on point-of-care testing (POCT) has significantly advanced. Electrochemical biosensors (ECBs) are one of the most promising candidates for the future of POCT due to their quick and accurate response, ease of operation, and cost effectiveness. This review focuses on the use of metal nanoparticles (MNPs) for fabricating ECBs that has a potential to be used for POCT. The field has expanded remarkably from its initial enzymatic and immunosensor-based setups. This review provides a concise categorization of the ECBs to allow for a better understanding of the development process. The influence of structural aspects of MNPs in biocompatibility and effective sensor design has been explored. The advances in MNP-based ECBs for the detection of some of the most prominent cancer biomarkers (carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), Herceptin-2 (HER2), etc.) and small biomolecules (glucose, dopamine, hydrogen peroxide, etc.) have been discussed in detail. Additionally, the novel coronavirus (2019-nCoV) ECBs have been briefly discussed. Beyond that, the limitations and challenges that ECBs face in clinical applications are examined and possible pathways for overcoming these limitations are discussed.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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“…AgNP aggregation-based electrochemical sensors were reported for determining Amyloid-β oligomers (AβOs) and gonadotropins [142][143] . Additionally, electrochemical sensors based on AgNP oxidation amplification and inhibition were used to assess T4 PNK, p53 protein, biomarker NS1, influenza virus H7, gastrodin, glucose, and DNA 113,119,142,[144][145][146][147] . Moreover, analyte-based electrochemical sensors using AgNPs as electrode modifiers were used to detect caffeine, creatinine, glucose, and estriol…”

Section: Electrochemical Detection and Characterization Of Agnpsmentioning

confidence: 99%

Recent advances in silver nanoparticle-based electrochemical sensors for determining organic pollutants in water: a review

et al. 2021

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“…AgNP networks formed in situ on the sensor electrode surface were utilized as electroactive reporters for signal readout. The biosensor is simple, cost-effective, and obviates the modification of nano-labels for molecular recognition [117]. Permual et al produced a green graphene nanofiber laser biosensor (LSG-NF) decorated with oil palm lignin-based synthetic AgNPs bonded with a single strand of DNA for the production of a tuberculosis bioelectrode to detect TB target DNA.…”

Section: Nanoparticlesmentioning

confidence: 99%

Technological advances in electrochemical biosensors for the detection of disease biomarkers

et al. 2021

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With an increasing focus on health in contemporary society, interest in the diagnosis, treatment, and prevention of diseases has grown rapidly. Accordingly, the demand for biosensors for the early diagnosis of disease is increasing. However, the measurement range of existing electrochemical sensors is relatively high, which is not suitable for early disease diagnosis, requiring the detection of small amounts of biocomponents. Various attempts have been made to overcome this and amplify the signal, including binding with various labeling molecules, such as DNA, enzymes, nanoparticles, and carbon materials. Efforts are also being made to increase the sensitivity of electrochemical sensors, and the combination of nanomaterials, materials, and biotechnology offers the potential to increase sensitivity in a variety of ways. Recent studies suggest that electrochemical sensors can be a powerful tool in providing comprehensive insights into the targeting and detection of disease-associated biomarkers. Significant advances in nanomaterial and biomolecule approaches for improved sensitivity have resulted in the development of electrochemical biosensors capable of detecting multiple biomarkers in real time in clinically relevant samples. In this review, we have discussed the recent studies on electrochemical sensors for detection of diseases such as diabetes, degenerative diseases, and cancer. Further, we have highlighted new technologies to improve sensitivity using various materials, including DNA, enzymes, nanoparticles, and carbon materials.

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Modification-free amperometric biosensor for the detection of wild-type p53 protein based on the in situ formation of silver nanoparticle networks for signal amplification

Cited by 31 publications

References 60 publications

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Recent advances in silver nanoparticle-based electrochemical sensors for determining organic pollutants in water: a review

Technological advances in electrochemical biosensors for the detection of disease biomarkers

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