MODIFICATION DE SURFACE DE POLY (HYDROXYBUTYRATE-CO-9% HYDROXYVALERATE) PAR POLYMERISATION PAR PLASMA d'ALCOOL ALLYLIQUE

Mas, André; Jaaba, H.; Schué, François; Belu, A. M.; Kassis, Camille M.; Linton, Richard W.; DeSimone, Joseph M.

doi:10.1016/s0014-3057(96)00154-1

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…The angle of contact of a sessile water drop was used to subjectively compare the hydrophobicity of untreated PHBV and AAc grafted PHBV. The advancing water contact angle of a solvent cast PHBV film was found to be 86° which compares well with literature values of 78−80° . After graft-co-polymerization with AAc (grafting yield of 3.0%), the water contact angle decreased to 60°.…”

Section: Resultssupporting

confidence: 85%

“…The advancing water contact angle of a solvent cast PHBV film was found to be 86°which compares well with literature values of 78-80°. 34 After graftco-polymerization with AAc (grafting yield of 3.0%), the water contact angle decreased to 60°. Considering that the water contact angle for PAAc 35 is 50°, we can conclude that a significant amount of the surface is functionalized with AAc in the modified film.…”

Section: Resultsmentioning

confidence: 99%

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Polymeric Grafting of Acrylic Acid onto Poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Surface Functionalization for Tissue Engineering Applications

2005

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Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) melt processed disks and solvent cast films were modified by graft co-polymerization with acrylic acid (AAc) in methanol solution at ambient temperature using gamma irradiation (dose rate of 4.5 kGy/h). To assess the presence of carboxylic acid groups on the surface, reaction with pentafluorophenol was performed prior to X-ray photoelectron spectroscopy analysis. The grafting yield for all samples increased with monomer concentration (2-15%), and for the solvent cast films, it also increased with dose (2-9 kGy). However, the grafting yield of the melt processed disks was largely independent of the radiation dose (2-8 kGy). Toluidine blue was used to stain the modified materials facilitating visual information about the extent of carboxylic acid functionalization and depth penetration of the grafted copolymer. Covalent linking of glucosamine to the functionalized surface was achieved using carbodiimide chemistry verifying that the modified substrates are suitable for biomolecule attachment.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Polymeric Grafting of Acrylic Acid onto Poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Surface Functionalization for Tissue Engineering Applications

2005

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“…To investigate the effect of grafting, XPS analysis on the surface was employed. The XPS multiplex scan of a solvent cast PHB sample shows a 1 : 1 : 1 ratio of CO : CO : C(CO) peaks as expected from the structure and in agreement with previous studies 35, 36. The C1s spectrum of cellulose was resolved into three components with different bonding state: the first at 285 eV assigned to CC or CH bond; the second at 286.8 eV assigned to CO bond; the third at 288 eV assigned to CO bond.…”

Section: Resultssupporting

confidence: 91%

Grafting biodegradable polyesters onto cellulose

Samain

Langlois

Renard

et al. 2011

J of Applied Polymer Sci

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To increase the compatibility between cellulose fibers and polyester matrix an original method for grafting hydrophobic oligoesters onto cellulose was proposed. Two kinds of cellulose substrates were employed as cellulose films and microcrystalline cellulose powder. Different oligoesters containing reactive end groups based on poly(DL-lactic acid) PDL-LA, poly(e-caprolactone) PCL and poly(3-hydroxyalkanoate)s PHA were first prepared and characterized by size exclusion chromatography (SEC), nuclear magnetic resonance (NMR), and differential scanning calorimetry (DSC). The carboxylic end groups of the polyesters were activated using thionyl chloride (SOCl 2 ) to increase the esterification reaction with the hydroxyl groups of the cellulose. The esterification was realized in a heterogenous medium without any catalyst by deposition of chloride oligoesters in solution (2-100 g L À1 ) onto cellulose film at different temperatures (25-105 C) during 1-12 h. The successful grafting on the various substrates was confirmed on the basis of FTIR spectroscopy, contact angle measurement, X-ray photoelectron spectroscopy (XPS) and thermogravimetric analysis (TGA). In particular, it is shown that a small quantity of grafted oligoesters led to a significant increase of the hydrophobic character of the cellulose with a contact angle near 130. The increase of hydrophobicity of cellulose is independent of the nature and length of grafting oligoesters.

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“…Supporting previous findings, we found PHBV to be more hydrophobic than TCP. 53 As a result, the adsorption of adhesion proteins to PHBV from the culture medium may have been inferior to their adsorption on TCP. This in turn may explain the observed differences between the substrates in cell attachment.…”

Section: Kumarasuriyar Et Almentioning

confidence: 99%

Poly(β-hydroxybutyrate-co-β-hydroxyvalerate) Supports in Vitro Osteogenesis

et al. 2005

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Studies have demonstrated that polymeric biomaterials have the potential to support osteoblast growth and development for bone tissue repair. Poly(beta-hydroxybutyrate-co-beta-hydroxyvalerate) (PHBV), a bioabsorbable, biocompatible polyhydroxy acid polymer, is an excellent candidate that, as yet, has not been extensively investigated for this purpose. As such, we examined the attachment characteristics, self-renewal capacity, and osteogenic potential of osteoblast-like cells (MC3T3-E1 S14) when cultured on PHBV films compared with tissue culture polystyrene (TCP). Cells were assayed over 2 weeks and examined for changes in morphology, attachment, number and proliferation status, alkaline phosphatase (ALP) activity, calcium accumulation, nodule formation, and the expression of osteogenic genes. We found that these spindle-shaped MC3T3-E1 S14 cells made cell-cell and cell-substrate contact. Time-dependent cell attachment was shown to be accelerated on PHBV compared with collagen and laminin, but delayed compared with TCP and fibronectin. Cell number and the expression of ALP, osteopontin, and pro-collagen alpha1(I) mRNA were comparable for cells grown on PHBV and TCP, with all these markers increasing over time. This demonstrates the ability of PHBV to support osteoblast cell function. However, a lag was observed for cells on PHBV in comparison with those on TCP for proliferation, ALP activity, and cbfa-1 mRNA expression. In addition, we observed a reduction in total calcium accumulation, nodule formation, and osteocalcin mRNA expression. It is possible that this cellular response is a consequence of the contrasting surface properties of PHBV and TCP. The PHBV substrate used was rougher and more hydrophobic than TCP. Although further substrate analysis is required, we conclude that this polymer is a suitable candidate for the continued development as a biomaterial for bone tissue engineering.

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MODIFICATION DE SURFACE DE POLY (HYDROXYBUTYRATE-CO-9% HYDROXYVALERATE) PAR POLYMERISATION PAR PLASMA d'ALCOOL ALLYLIQUE

Cited by 11 publications

References 6 publications

Polymeric Grafting of Acrylic Acid onto Poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Surface Functionalization for Tissue Engineering Applications

Polymeric Grafting of Acrylic Acid onto Poly(3-hydroxybutyrate-co-3-hydroxyvalerate): Surface Functionalization for Tissue Engineering Applications

Grafting biodegradable polyesters onto cellulose

Poly(β-hydroxybutyrate-co-β-hydroxyvalerate) Supports in Vitro Osteogenesis

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