Modification and Exploitation of a Poliovirus-induced Membrane Complex by Superinfecting ME Virus

Abstract

SUMMARYThe replication of Mouse Elberfeld (ME) virus was accelerated when HEp-2 cells were mixedly infected with poliovirus in the presence of guanidine. The latent period of the replication of ME virus was shortened by 3 h when cells were preinfected for at least 2 h with poliovirus and inhibited by guanidine. Simultaneous infection with poliovirus and ME virus resulted in a shortening by 1 h of the latent period of ME virus replication. The accelerated replication of ME virus was shown to be due to modification and exploitation of a membrane complex induced by poliovirus in the presence of guanidine; on superinfection ME virus successively modified this potiovirus-induced complex of 470S ('light' complex) into a 'heavy' complex of 700S specific for ME virus.

A Poliovirus-induced Cytoplasmic Membrane Complex is Exploited by the RNA Polymerase of Superinfecting Mouse Elberfeld (ME) Virus

“…complementation does not take place between ME and poliovirus reproduction. As we were able to show recently (Zeichhardt et al, 1981), poliovirus inhibited by guanidine, nevertheless, induces a cytoplasmic membrane complex of 470S (Fig. 1).…”

“…Under these conditions non-reproducing ME virus determines the membrane changes at the cell surface (Zeichhardt et al, 1982). (ii) In a mixed infection in the presence of guanidine, poliovirus reproduction is inhibited (Baltimore et al, 1963); however, reproduction of ME virus is advanced (Diefenthal et al, 1973;Zeichhardt et al, 1981). The reproduction of ME virus is accelerated by 1 h in a simultaneous mixed infection and by as much as 3 h when the cells are preinfected with poliovirus for at least 2 h. Under these conditions poliovirus is not rescued by superinfecting ME virus, i.e.…”

“…---, Separation profile of homogenates of cells preinfected with poliovirus for 4 h and superinfected with ME virus for 6 h, with guanidine present throughout infection (P_4ME6 + G); modification of the preexisiting 470S complex into an ME virus-specific membrane complex of 700S. A single infection with ME virus for 8 h also leads to the formation of a 700S complex (not shown, see Zeichhardt et al, 1981).…”

“…Growth of HEp-2 cells in monolayer cultures, propagation of poliovirus type 1 (strain Mahoney) and ME virus (laboratory strain), double infection experiments with both viruses and separation of cytoplasmic membrane complexes were performed as described previously (Zeichhardt et aL, 1981). In brief, the conditions for superinfection were chosen according to the kinetics of reproduction of ME virus in poliovirus-preinfected cells.…”

“…From this it has been concluded that ME virus is reproduced with a shortened latent period by exploiting the 'poliovirus/guanidine'-induced and preexisting membrane complex for its own reproduction. The present communication shows that the modified complex serves as a site for ME virusspecific RNA synthesis.Growth of HEp-2 cells in monolayer cultures, propagation of poliovirus type 1 (strain Mahoney) and ME virus (laboratory strain), double infection experiments with both viruses and separation of cytoplasmic membrane complexes were performed as described previously (Zeichhardt et aL, 1981). In brief, the conditions for superinfection were chosen according to the kinetics of reproduction of ME virus in poliovirus-preinfected cells.…”