Modificação da técnica de alotransplante pulmonar unilateral em ratos<A HREF="#tit1">*</A>

Abstract

The increasing demand in transplantation research requires efficient and less expensive animal models in order to obtain reliable results that are reproducible in larger animal models and, ultimately, applied clinically. The model of unilateral left lung transplantation in rats has proven to be a useful alternative for those purposes. We demonstrate a technical modification of this model, which consists of the isolation and ligation of the contralateral (right) pulmonary artery, allowing blood circulation exclusively in the transplanted lung. This model is feasible and reproducible. However, the short survival time restricts the assessment of the transplanted lung to a maximum period of three hours.

Endobronchial Perfluorocarbon Reduces Inflammatory Activity before and after Lung Transplantation in an Animal Experimental Model

“…The animals were prepared for pulmonary perfusion as described elsewhere [28]. Preservation was performed using 20 mL of low-potassium dextran glucose preservation solution (LPD) at 4°C via antegrade cannulation of the pulmonary artery with drainage of the effluent via the left atrium.…”

“…Orthotopic left lung transplantation was performed using the modified cuff technique we previously described [28]. Briefly, after general anesthesia with intraperitoneal ketamine (100 mg/kg) and xylazine (15 mg/kg), orotracheal intubation, mechanical ventilation, anticoagulation (300 U heparin), and median sternolaparotomy, the donor rat lungs were submitted to an antegrade flush via the pulmonary artery with 20 mL of cold (4°C) LPD solution at a pressure of 20 cm H 2 O.…”

Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation

“…Two hours following brain death, the donor rat lungs were flushed in an antegrade manner with 20 ml of cold (4°C), low-potassium dextran solution (LPD-Perfadex®; Vitrolife, Göteborg, Sweden) at a pressure of 20 cmH 2 O via the pulmonary artery, as described elsewhere 10. The heart-lung block was extracted with the lungs inflated at the end-tidal volume.…”

“…Then, they underwent a left thoracotomy, and the pulmonary vessels and left bronchus were anastomosed using a standard cuff technique 10. The arterial clamps were released approximately 3 h after harvest.…”

Ferret Lung Transplant: An Orthotopic Model of Obliterative Bronchiolitis

“…The ferret left lung vasculature anatomy makes it technically challenging to implant the upper lobe and because the lower lobe is large, we implanted only the lower lobe. The arterial and venous cuffs were prepared as described for rodent models (17), using 12-gauge intravenous angiocatheters. The donor lung was kept covered in Perfadex-soaked gauze, in a sterile petri dish over ice.…”