Modestobacter lapidis sp. nov. and Modestobacter muralis sp. nov., isolated from a deteriorated sandstone historic building in Salamanca, Spain

Abstract

A polyphasic study was undertaken to establish the taxonomic status of two Modestobacter strains isolated from the surface of deteriorated sandstone of a historic building in Salamanca, Spain. The strains, isolates MDVD1(T) and MON 3.1(T), were found to have chemotaxonomic and morphological properties consistent with their classification in the genus Modestobacter and to form distinct phyletic lines in the Modestobacter 16S rRNA gene tree. Isolate MDVD1(T) was found to be closely related to the type strain of Modestobacter versicolor (98.7 % similarity) and isolate MON 3.1(T) to the type strain of Modestobacter multiseptatus (98.6 % similarity). The isolates were distinguished readily from one another and from the Modestobacter type strains by a broad range of phenotypic properties, by qualitative and quantitative differences in fatty acid profiles and by BOX fingerprint patterns. On the basis of these data, it is proposed that the isolates be classified in the genus Modestobacter as Modestobacter lapidis sp. nov. and Modestobacter muralis sp. nov., with isolates MON 3.1(T) (CECT 8844(T) = DSM 100206(T)) and MDVD1(T) (CECT 8845(T) = DSM 100205(T)) as the respective type strains.

Modestobacter altitudinis sp. nov., a novel actinobacterium isolated from Atacama Desert soil

“…The chemotaxonomic, cultural and morphological properties of the isolates were consistent with their classification in the genus Modestobacter [1][2][3][4]8]. The isolates were aerobic, Gram-stain-positive, non-motile, formed short rods and coccoid-shaped cells which tended to remain aggregated ( The isolates grew particularly well on ISP media 2 and 6 producing black colonies on the former and dark brown or light-olive ones the latter (Table 1).…”

“…It is worth mentioning that in previously studies [8,10,24] glycophosphatidylinositol (GPI) was annotated as phosphatidylinositol mannoside (PIM); this compound has been found in representatives of Modestobacter species, apart from M. multiseptatus [1,4]. Consequently, the polar lipid profiles of isolates 1G4 T , 1G51 and 1G52 accord with those found in the Maximum-likelihood (ML) and maximum-parsimony (MP) phylogenetic trees based on the 16S rRNA gene sequences were inferred using the genome-to-genome distance calculator (GGDC) web server [39] adapted to single genes and multiple sequence alignments generated using ClustalW [40].…”

“…Table 1. Continued [4,8], Modestobacter marinus from deep-sea sediment [9] and Modestobacter caceresii and 'Modestobacter excelsi' from hyper-arid Atacama Desert soils [3,10]. Culture-independent studies show that Modestobacter strains are key components of Atacama Desert soils [11,12] characterised by scarcity of liquid water, low concentrations of organic carbon and high solar irradiation [13][14][15].…”

“…The isolates were examined for chemotaxonomic, cultural and morphological properties known to be of value in the classification of Modestobacter species [3,4]. Motility and micromorphological features were acquired according to Trujillo et al [8] and cultural properties recorded from tryptone-yeast extract, yeast extract-malt extract, oatmeal, inorganic-salts starch, glucose-asparagine, peptone-yeast extract-iron and tyrosine agar (International Streptomyces Project [ISP] media 1-7 respectively [26]) plates following incubation at 28 °C for 14 days; colony colours were determined by comparison against colour charts [27]. In turn, chemotaxonomic properties of the isolates were established using standard procedures.…”

Draft Genome Sequence ofModestobactersp. strain VKM Ac-2676 from soil in the area of sharply continental climate

“…The genus Modestobacter (family Geodermatophilaceae , phylum “Actinobacteria”) includes six validly described species (LPSN; http://www.bacterio.net) (1). A seventh species has recently been proposed (2).…”