Modern Synthetic Avenues for the Preparation of Functional Fluorophores

Abstract

Biomedical research relies on the fast and accurate profiling of specific biomolecules and cells in a non‐invasive manner. Functional fluorophores are powerful tools for such studies. As these sophisticated structures are often difficult to access through conventional synthetic strategies, new chemical processes have been developed in the past few years. In this Minireview, we describe the most recent advances in the design, preparation, and fine‐tuning of fluorophores by means of multicomponent reactions, C−H activation processes, cycloadditions, and biomolecule‐based chemical transformations.

Multiple Multicomponent Reactions: Unexplored Substrates, Selective Processes, and Versatile Chemotypes in Biomedicine

“…The need for novel functional fluorophores has prompted the development of new synthetic strategies to prepare probes not accessible through classical synthesis, and MCRs constitute a valuable platform to afford them . The versatility of the novel multiple GBBRs allows for the fine tuning of the structural and spectral properties of the adducts.…”

SCOTfluors: Small, Conjugatable, Orthogonal, and Tunable Fluorophores for In Vivo Imaging of Cell Metabolism

“…[*] S. Benson, [+] Several strategies have been described to optimize the optical properties of fluorophores for live-cell imaging. [5] For instance,t he replacement of oxygen atoms with geminal dimethyl groups in rhodamine and fluorescein produced redshifted fluorophores with enhanced properties for bioimaging. [6] We envisioned that the synthesis of nitrobenzodiazoles with different groups bridging the nitroaminoaniline core would render multi-color fluorophores with tunable emission and enhanced capabilities for metabolite imaging in live cells.…”

Ikusgai-eremu elektromagnetiko osoan zehar igorpen sintonizagarria duten BODIPY laser-koloratzaileak

“…Kromoforo hauen arrakasta sintesi organikoan [1] eta bereizmen handiko teknika espektroskopikoetan [2] izandako aurrerapausoei esker sustatu da. Esaterako, fluoreszentzia-irudiekin erlazionatutako tekniken aurrerapen teknologikoak direla eta, koloratzaileak markatzaile fluoreszente modura asko erabiltzen dira (1. irudia) [3].…”