Modern Chromatographic Methods for the Identification and Quantification of Plant Growth Regulators and Their Application to Studies of the Changes in Hormonal Substances in Winter Wheat During Acclimation to Cold Stress Conditions

Wightman, F.

doi:10.1007/978-1-4684-3512-2_19

Cited by 22 publications

(13 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the study here, the maximum increase observed was a 1.5-to 3-fold increase in the ABA level of plants exposed to 10/5 C; nevertheless, the increase under high or low temperature regimes is consistent and significant. Similar increases in free ABA levels were observed in the leaves and crown tissue of wheat plants subjected to gradually decreasing diurnal temperature regimes (25). Therefore, it seems that temperature stress, per se, causes an increase in ABA levels of plants.…”

Section: Resultssupporting

confidence: 66%

“…Minimal and constant total ABA levels were also observed in plants exposed to 25/15 C for all sampling times (Fig. 3).…”

Section: Resultsmentioning

confidence: 70%

“…With the exception of the 12-h sampling, these values were significantly lower than the free ABA content of plants exposed to other temperatures. At 12 h, the free ABA contents of plants at 25 Results of the effect of temperature on conjugated ABA are presented in Figure 2. As with free ABA, lowest conjugated ABA levels were observed at 25/15 C. Conjugated ABA levels in plants subjected to 15/10 and 10/5 C continued to increase during the course of the experiment, the maximum being a 2-fold increase at 24 h, and an increase during the latter part of the experiment.…”

Section: Resultsmentioning

confidence: 99%

“…These elevated ABA levels under temperature stress may have important physiological implications (18-20, 25, 26). Wightman (25) reported that, when wheat plants were subjected to decreasing diurnal temperature conditions over a 5-week period, ABA levels were increased to 3-and 10-fold, depending on the variety. Itai et al (10) presented evidence that heat treatment increased the ABA levels in the xylem exudate of bean plants.…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Response of Tomato Plants to Stressful Temperatures

Daie¹,

Campbell²

1981

Plant Physiol.

165

View full text Add to dashboard Cite

To investigate the abscisic acid (ABA) production of tomato (Mill.) plants in response to diurnal stressful temperatures, five-week old seedlings were exposed to day/night temperatures of 10/5, 15/10, 25/15, 35/25, or 45/35 C. The daylength was 16 hours with a light intensity of approximately 400 microeinsteins per meter per second. Plant tops were sampled at 12, 24, 48, and 72 hours. Free, alkaline-hydrolyzable (conjugated), and total ABA quantities were measured using standard gas chromatographic techniques. AU temperature regimes significantly increased both free and conjugated ABA levels over concentrations in control plants (25/15 C). The highest ABA levels were observed in plants exposed to the coolest temperature of 10/5 C. Since normal water potentials were obtained in plants of al treatments, the observed ABA response was not due to temperature-induced water stress. Therefore, temperature stress, like several other environmental stresses, induces the plant to produce high levels of ABA. Because of the similar involvement of ABA in temperature-induced and other environmental stresses, ABA may be a common mediator for all plant stresses.ABA is a naturally occurring compound of major importance in regulating plant growth and development. It has been implicated in a variety of physiological processes (1,11,13,15) and is found in elevated levels under several stressful conditions (3,4,7,8,16,18,23 Samples for ABA determination were harvested at 0, 12, 24, 48, and 72 h. Zero sampling time (09:00 h) was 3 h after the lights came on; therefore, all samples, except those at 12 h, were harvested 3 h after the lights came on. The 12-h samples were taken 1 h before the end of the light period (2 1:00 h). At each harvest, three single plant replicates (exclusive of the root system) were immediately frozen on dry ice and stored in a freezer at -18 C until analyzed. A representative sample (1-3 g) of each frozen and crushed plant was weighed, homogenized in ice-cold 90% methanol (10 ml/g fresh tissue), and filtered. ABA was analyzed according to the method described by Seeley and Powell (21). The alkaline-hydrolyzable ABA (conjugated ABA) was determined by adjusting the pH of the remaining aqueous phase to 11.0 with KOH, heating it at 60 C for 45 min, and re-extracting with methylene chloride. Acidic fractions were derivatized by ethereal diazomethane. ABA was quantified with a Tracor 222 Gas Chromatograph equipped with a Ni3 electron capture detector. Column packing was 3% OV-25 on Gas Chrom Q (100 to 200 mesh support). Purified N2 at a flow rate of 80 ml/min was used as the carrier gas.

show abstract

Section: Resultssupporting

confidence: 66%

“…Minimal and constant total ABA levels were also observed in plants exposed to 25/15 C for all sampling times (Fig. 3).…”

Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Response of Tomato Plants to Stressful Temperatures

Daie¹,

Campbell²

1981

Plant Physiol.

165

View full text Add to dashboard Cite

show abstract

“…This hypothesis is based on three experimental observations. First, the endogenous levels of ABA increase when plants are exposed to low, hardening temperatures (17). Second, the exogenous application of ABA as a foliar spray or in hydroponic solution increases the hardiness of some plant species (1, 1 1), although others apparently do not respond (7).…”

mentioning

confidence: 99%

The Effect of Abscisic Acid on the Freezing Tolerance of Callus Cultures of Lotus corniculatus L.

Keith¹,

McKersie²

1986

Plant Physiol.

View full text Add to dashboard Cite

ABSTRACIThe effects ofgrowth temperature (2°C and 24C), abscisic acid (ABA) concentration, duration of exposure to ABA, and light were assessed for their ability to induce acclimation to freezing temperatures in callus cultures of Lotus corniculatus L. cv Leo, a perennial forage legume. The maximal expression of freezing tolerance was achieved on B% media containing 10 molar ABA, at 24°C for 7 or 14 days. Under these culture conditions, the freezing tolerance of the callus approximated that observed in field grown plants. In contrast, low temperatures (2°C) induced only a limited degree of freezing tolerance in these cultures. Viability was assessed by tetrazolium reduction and by regrowth of the callus. The two assays often differed in their estimates of absolute freezing tolerance. Regression analysis of the temperature profile suggested that there may be two or more distinct populations of cells differing in freezing tolerance, which may have contributed to the variability between viability assays.Winter annual and perennial plants have the ability to acclimate to freezing stress in response to environmental signals such as low temperature or short daylength (4). Since the acclimation process is associated with a period of slow growth and often dormancy, it has been proposed that the induction of freezing tolerance may be mediated by shifts in the hormonal balance, specifically in the levels of ABA (9). This hypothesis is based on three experimental observations. First, the endogenous levels of ABA increase when plants are exposed to low, hardening temperatures (17). Second, the exogenous application of ABA as a foliar spray or in hydroponic solution increases the hardiness of some plant species (1, 1 1), although others apparently do not respond (7). Third, the inclusion of ABA in the culture media induces freezing tolerance in both potato callus (1) Tomes. They were originally produced from the axillary buds of plants that were selected on the basis of their vigor and performance in culture. Callus cultures were grown according to the method of Swanson and Tomes (15) and 100 x 20 mm Petri dishes in a 16 h photoperiod at 35 ,uE m-2 s-' supplied by General Electric warm white fluorescent lights. The callus was subcultured every 3 weeks onto B5 media (5) containing 8 g L' agar, 1 mg L-' 2,4-D, and without kinetin. When included, ABA was added from a lOx stock solution prepared by initially dissolving ABA in 4 ml ethanol and diluting to 100 ml with distilled H20. The other nutrients were added from stock solution dissolved in distilled H20.Acclimation and Application of Freezing Stress. Approximately 0.5 g of callus from the stock culture was placed on a sheet of Whatman No. 50 fiber filter paper in a 60 x 20 mm Petri dish that contained a layer of B5 agar media, either with or without ABA. Following the required acclimation treatment, the callus and filter paper were transferred to a Petri dish that contained a layer of moistened sterile sand, covered by a second sheet of filter paper. The moistened layer of ...

show abstract

Water-in-Plants Bibliography, volume 5 1979

Pospíšilová¹,

Solárová²

1980

View full text Add to dashboard Cite

Modern Chromatographic Methods for the Identification and Quantification of Plant Growth Regulators and Their Application to Studies of the Changes in Hormonal Substances in Winter Wheat During Acclimation to Cold Stress Conditions

Cited by 22 publications

References 82 publications

Response of Tomato Plants to Stressful Temperatures

Response of Tomato Plants to Stressful Temperatures

The Effect of Abscisic Acid on the Freezing Tolerance of Callus Cultures of Lotus corniculatus L.

Water-in-Plants Bibliography, volume 5 1979

Contact Info

Product

Resources

About