Modern approaches to the production of inactivated vaccines against chicken cholera

Rozhdestvenskaya, T.N.; Karimova, L.; Pankratov, S. V.; Ruzina, Anna; Tomina, E.V.

doi:10.32634/0869-8155-2022-361-7-8-68-73

Cited by 3 publications

(3 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Однако на данный момент наряду с достигнутыми положительными результатами, связанными с эффективностью применения противобактериальных инактивированных вакцин в промышленном птицеводстве, зачастую существует проблема с проявлением их остаточной реактогенности. Данную проблему во многом можно решить с помощью использования в изготовление вакцин современных масляных адъювантов нового поколения [7].…”

Section: противобактериальные вакцины для птиц изготовленные на основ...unclassified

“…Бесспорно, проблему реактогенности инактивированных противобактериальных вакцин во многом можно решить за счет применения современных масляных адъювантов нового поколения, но это, к сожалению, не всегда позволяет решить вопрос в полной мере. В тоже время реактогенность противобактериальных вакцин можно существенно снизить за счет использования в изготовлении препаратов очищенных антигенных комплексов бактериальных клеток без содержания каких-либо «балластных» клеточных структур микробной клетки, которые не участвуют в формировании иммунного ответа к возбудителю болезни [7]. The use of antimicrobial drugs and vaccinoprophylaxis are the main ways to prevent and combat most bacterial diseases.…”

Section: противобактериальные вакцины для птиц изготовленные на основ...unclassified

See 1 more Smart Citation

Antibacterial vaccines for poultry made on the basis of the adjuvant ICTYOLANE<sup>TM</sup> 11

Pankratov

2024

jour

Self Cite

View full text Add to dashboard Cite

The use of antimicrobial drugs and vaccinoprophylaxis are the main ways to prevent and combat most bacterial diseases. However, the unsystematic use of antimicrobials without taking into account the sensitivity of pathogens to drugs often does not allow achieving the desired results. On the other hand, the use of a properly selected vaccine, taking into account the epizootic situation in the farm, is one of the safe and effective tools for controlling diseases of bacterial etiology. In this regard, the results presented in this article of testing samples of vaccines against bacterial diseases of birds, made on the basis of a modern oil adjuvant ICTYOLANETM 11, are interesting and timely. For research three vaccine samples were manufactured based on the oil adjuvant ICTYOLANETM 11. The first sample of the vaccine is against avian salmonellosis, the second is against avian pasteurellosis and the third is against avian respiratory mycoplasmosis. Analysis of the results showed that all vaccine samples made on the basis of the oil adjuvant ICTYOLANETM 11 met the specified parameters in terms of viscosity and stability, ensured the formation of humoral immunity of the required level and fully met the requirements for drugs of this class. But along with good physico-chemical and immunological indicators, vaccines against salmonellosis and pasteurellosis of birds showed reactogenic properties to one degree or another, while the vaccine against respiratory mycoplasmosis of birds was areactogenic. Based on the obtained research results, it can be concluded that the use of the adjuvant ICTYOLANETM 11 in the production of a vaccine against avian respiratory mycoplasmosis makes it possible to obtain a safe and effective immunobiological preparation.

show abstract

Section: противобактериальные вакцины для птиц изготовленные на основ...unclassified

Antibacterial vaccines for poultry made on the basis of the adjuvant ICTYOLANE<sup>TM</sup> 11

Pankratov

2024

jour

Self Cite

View full text Add to dashboard Cite

show abstract

“…ИФА занял прочное место в лабораторной диагностики пастереллеза птиц. Так тест системы ИФА производства IDEXX, BioChek и ID.vet достаточно широко используются в промышленном птицеводстве и являются незаменим инструментом для серологической диагностики пастереллеза птиц, а также определения уровня поствакцинального иммунитета и эффективности проведенной вакцинации птиц [2,6].…”

Section: результаты и обсуждениеunclassified

Modern approaches in the diagnosis of avian Pasteurellosis

Pankratov

Abgaryan

2023

jour

Self Cite

View full text Add to dashboard Cite

Studies carried out in many countries of the world demonstrate considerable variability in the virulence properties of pasteurellosis. The variety of antigenicity and toxicity serovars of the pathogen determines a number of specific features of display of poultry pasteurellosis, especially in associated infections when several causative agents of infectious diseases of bacterial and/or viral etiology circulate in one farm. In mixed infections, birds show a variety of clinical signs, most notably a respiratory syndrome characterized by respiratory involvement (sinusitis, conjunctivitis, laryngitis, tracheitis, bronchitis, pneumonia, and aerosacculitis) and tissue swelling in the suborbital sinuses, intermandibular spaces, and ear flaps.This situation makes it difficult to timely and promptly conduct effective therapeutic and anti-epizootic measures. However, it is necessary to identify the etiology of the disease as soon as possible, methodically and comprehensively, taking into account epizootic data, clinical signs, pathological anatomical changes and with obligatory confirmation by laboratory tests.At present time laboratory methods for diagnostics of avian pasteurellosis are regulated by Methodical instructions (MI) on laboratory diagnostics of pasteurellosis of animals and birds approved by the Chief Veterinary Department on August 20, 1992 № 22-7/82.According to the approved MI, the laboratory diagnosis of avian pasteurellosis includes microscopy of smears and fingerprints, isolation of pasteurella cultures and their identification, and, if necessary, bioassaying.It should be noted that the laboratory diagnosis of pasteurellosis according to the MI is a laborious and time-consuming process.Apart from the methods described in the MI, the serological method - enzyme immunoassay (ELISA) and molecular biological method - polymerase chain reaction (PCR) are currently widely and successfully used in laboratory practice for diagnosis of avian pasteurellosis, which are not reflected in the approved MI, but allow relatively simple and rapid reliable results.

show abstract

Use of MONTANIDE ISA 78 VG as adjuvant for the manufacture of antibacterial vaccines for chickens

Pankratov

2024

Agrarnaâ nauka

View full text Add to dashboard Cite

Relevance. The use of antimicrobial agents and vaccine prophylaxis are the main ways to prevent and control most diseases of bacterial etiology. At the same time, uncontrolled use of antimicrobial agents without determining the sensitivity of microorganisms, as a rule, does not give the opportunity to obtain a positive therapeutic result. At the same time, a competently designed scheme of inactivated vaccine administration, taking into account the epizootic situation, is an effective and safe tool for controlling bacterial diseases.In this regard, the results of testing of vaccine samples against bacterial diseases of birds based on the modernized oil adjuvant Montanide ISA 78 VG presented in this article are interesting and modern.Methods. Four vaccine samples were manufactured for research based on the oil adjuvant Montanide ISA 78 VG. The first sample of the vaccine is against avian salmonellosis, the second — against avian pasteurellosis, the third — against avian respiratory mycoplasmosis, the fourth — against avian hemophilosis. All manufactured vaccine samples were investigated for sterility, stability and viscosity of the emulsion according to generally accepted methods. To determine the reactogenicity and antigenic activity of the vaccine samples, young egg-laying hens aged 25–40 days were used.Results. Analysis of the obtained results of physical and biological properties of tested inactivated vaccines against bacterial diseases of birds allows us to conclude that the vaccine against respiratory mycoplasmosis of birds made on the basis of adjuvant Montanide ISA 78 VG is a safe and effective immunobiological preparation.

show abstract

Modern approaches to the production of inactivated vaccines against chicken cholera

Cited by 3 publications

References 14 publications

Antibacterial vaccines for poultry made on the basis of the adjuvant ICTYOLANE<sup>TM</sup> 11

Antibacterial vaccines for poultry made on the basis of the adjuvant ICTYOLANE<sup>TM</sup> 11

Modern approaches in the diagnosis of avian Pasteurellosis

Use of MONTANIDE ISA 78 VG as adjuvant for the manufacture of antibacterial vaccines for chickens

Contact Info

Product

Resources

About