Moderate SMFs attenuate bone loss in mice by promoting directional osteogenic differentiation of BMSCs

Abstract

Background Osteoporosis is a common metabolic bone disease without effective treatment. Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to differentiate into multiple cell types. Increased adipogenic differentiation or reduced osteogenic differentiation of BMSCs might lead to osteoporosis. Whether static magnetic fields (SMFs) might influence the adipo-osteogenic differentiation balance of BMSCs remains unknown. Methods The effects of SMFs on lineage differentiation of BMSCs and development of osteoporosis were determined by various biochemical (RT-PCR and Western blot), morphological (staining and optical microscopy), and micro-CT assays. Bioinformatics analysis was also used to explore the signaling pathways. Results In this study, we found that SMFs (0.2–0.6 T) inhibited the adipogenic differentiation of BMSCs but promoted their osteoblastic differentiation in an intensity-dependent manner. Whole genomic RNA-seq and bioinformatics analysis revealed that SMF (0.6 T) decreased the PPARγ-mediated gene expression but increased the RUNX2-mediated gene transcription in BMSCs. Moreover, SMFs markedly alleviated bone mass loss induced by either dexamethasone or all-trans retinoic acid in mice. Conclusions Taken together, our results suggested that SMF-based magnetotherapy might serve as an adjunctive therapeutic option for patients with osteoporosis.

Iron Oxide Nanoparticles Combined with Static Magnetic Fields in Bone Remodeling

“…An SMF of 15 mT promoted proliferation, ALP activity, and mineralized nodule formation in hBMSCs in a time-dependent manner and upregulated the expression of osteogenic marker genes, indicating that 15 mT SMF promotes osteogenic differentiation and biomineralization in hBMSCs [ 50 ]. Similarly, osteoblastogenesis was also enhanced by an SMF of 0.2, 0.4, and 0.6 T in BMSCs from rats or mice [ 51 , 52 ]. Zheng et al [ 53 ] showed that an SMF of 1 mT increased cell proliferation, osteogenic differentiation, and biomineralization in hDPSCs.…”

Epigenetic therapy targeting bone marrow mesenchymal stem cells for age-related bone diseases

“…SMF (0.2–0.6 T) promotes BMSC osteogenesis and inhibits adipogenic differentiation in an intensity-dependent manner. RNA-seq analysis of the entire genome in BMSCs has shown that SMF (0.6 T) could reduce PPARγ expression and increase RUNX2 transcription [ 115 ]. Moreover, recent research also reported a novel and effective strategy, namely nanosecond pulsed electric fields (nsPEFs) stimulation.…”

Effect of Static Magnetic Field on Bone Marrow Cellular Density

“…Whereas, the 180 mT has reduced the bone mineral densities significantly in distal regions (Xu, et al, 2010). Osteoporosis also examined under the influence of moderate 0.2-0.6 mT magnetic field (Chen, et al, 2020). It was found that the magnetic field inhibits the adipogenic differentiation of bone marrowderived mesenchymal stem cells depends on the intensity of exposure.…”