Models for Monocytic Cells in the Tumor Microenvironment

Lee, Sharon Wei Ling; Adriani, Giulia; Kamm, Roger D.; Gillrie, Mark R.

doi:10.1007/978-3-030-35723-8_7

Cited by 8 publications

(5 citation statements)

References 296 publications

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“…different cell migration-inducing mechanisms that rely on biochemical, mechanical and topographical properties of the surrounding environment [19]- [22]. At the same time, a better understanding of the mechanisms by which cells migrate may lead to the development of novel therapeutic strategies for controlling tumor invasiveness and metastasis, mainly known as migrastatic drugs [23].…”

Section: Accepted Manuscript N O T C O P Y E D I T E Dmentioning

confidence: 99%

Confined Cell Migration and Asymmetric Hydraulic Environments to Evaluate the Metastatic Potential of Cancer Cells

Juste-Lanas

Guerrero

Camacho-Gómez

et al. 2022

Journal of Biomechanical Engineering

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Metastasis, a hallmark of cancer development, is also the leading reason for most cancer-related deaths. Furthermore, cancer cells are highly adaptable to microenvironments and can migrate along pre-existing channel-like tracks of anatomical structures. However, more representative three-dimensional models are required to reproduce the heterogeneity of metastatic cell migration in vivo to further understand the metastasis mechanism and develop novel therapeutic strategies against it. Here, we designed and fabricated different microfluidic-based devices that recreate confined migration and diverse environments with asymmetric hydraulic resistances. Our results show different migratory potential between metastatic and nonmetastatic cancer cells in confined environments. Moreover, although nonmetastatic cells have not been tested against barotaxis due to their low migration capacity, metastatic cells present an enhanced preference to migrate through the lowest resistance path, being sensitive to barotaxis. This device, approaching the study of metastasis capability based on confined cell migration and barotactic cell decisions, may pave the way for the implementation of such technology to determine and screen the metastatic potential of certain cancer cells.

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Section: Accepted Manuscript N O T C O P Y E D I T E Dmentioning

confidence: 99%

Confined Cell Migration and Asymmetric Hydraulic Environments to Evaluate the Metastatic Potential of Cancer Cells

Juste-Lanas

Guerrero

Camacho-Gómez

et al. 2022

Journal of Biomechanical Engineering

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“…[21][22][23][24] Among the existing 3D culture platforms, microfluidic systems represent a promising tool to recreate the spatial tissue architecture in a highly controllable fashion to recapitulate the in vivo microenvironment and test immunotherapeutic strategies. [25][26][27] 3D microfluidic models allow a real-time analysis of cellular interactions, the possibility to mimic the main steps of the metastatic cascade and elucidate critical factors in the tumor progression. [28][29][30][31][32][33] For instance, the involvement of macrophages in regulating the intravasation of breast cancer cells through an endothelial layer when co-cultured in a microfluidic device was demonstrated by Zervantonakis et al 34 In a microfluidic-based lung carcinoma model, Bai et al investigated the contribution of tumor-associated macrophages (TAMs) in modulating the epithelial-to-mesenchymal transition (EMT) of cancer cell aggregates.…”

Section: Introductionmentioning

confidence: 99%

A 3D pancreatic tumor model to study T cell infiltration

et al. 2021

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“…Nonetheless, the performance in prolonged survival depends on the individual immune microenvironment [ 34 ]. Tumor-infiltrating monocytes are critical regulators in the tumor immune microenvironment, modulating tumor growth and metastasis [ 35 , 36 ]. The activity depends on the plasticity of monocytes in response to the stimuli of TME.…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Prognostic Biomarkers Associated with Monocyte Infiltration in Lung Squamous Cell Carcinoma

Liu

Yan

Chen

et al. 2022

BioMed Research International

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The five-year survival rate of lung squamous cell carcinoma is significantly lower than that of other cancer types. It is therefore urgent to discover novel prognosis biomarkers and therapeutic targets and understand their correction with infiltrating immune cells to improve the prognosis of patients with lung squamous cell carcinoma. In this study, we employed robust rank aggregation algorithms to overcome the shortcomings of small sizes and potential bias in each Gene Expression Omnibus dataset of lung squamous cell carcinoma and identified 513 robust differentially expressed genes including 220 upregulated and 293 downregulated genes from six microarray datasets. Functional enrichment analysis showed that these robust differentially expressed genes were obviously involved in the extracellular matrix and structure organization, epidermis development, cell adhesion molecule binding, p53 signaling pathway, and interleukin-17 signaling pathway to affect the progress of lung squamous cell carcinoma. We further identified six hub genes from 513 robust differentially expressed genes by protein-protein interaction network and 10 topological analyses. Moreover, the results of immune cell infiltration analysis from six integrated Gene Expression Omnibus datasets and our sequencing transcriptome data demonstrated that the abundance of monocytes was significantly lower in lung squamous cell carcinoma compared to controls. Immune correlation analysis and survival analysis of hub genes suggested that three hub genes, collagen alpha-1(VII) chain, mesothelin, and chordin-like protein 1, significantly correlated with tumor-infiltrating monocytes as well as may be potential prognostic biomarkers and therapy targets in lung squamous cell carcinoma. The investigation of the correlation of hub gene markers and infiltrating monocytes can also improve to well understand the molecular mechanisms of lung squamous cell carcinoma development.

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Models for Monocytic Cells in the Tumor Microenvironment

Cited by 8 publications

References 296 publications

Confined Cell Migration and Asymmetric Hydraulic Environments to Evaluate the Metastatic Potential of Cancer Cells

Confined Cell Migration and Asymmetric Hydraulic Environments to Evaluate the Metastatic Potential of Cancer Cells

A 3D pancreatic tumor model to study T cell infiltration

Identification of Potential Prognostic Biomarkers Associated with Monocyte Infiltration in Lung Squamous Cell Carcinoma

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