Models for matrix-assisted desorption by a laser-pulse

Johnson, Russell B.

doi:10.1016/0168-1176(94)04070-2

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Desorption is mainly induced by sudden heating of matrix crystals during laser excitation. Desorption processes in MALDI have been described quantitatively in more detail than ionization [40][41][42][43]. The desorption efficiency of materials depends on the intrinsic properties of the samples and the excitation conditions, such as the molecular weight [43], crystal morphology [44][45][46] and laser conditions [5,[47][48][49].…”

Section: (B) Desorptionmentioning

confidence: 99%

Critical factors determining the quantification capability of matrix-assisted laser desorption/ionization– time-of-flight mass spectrometry

Wang

Lai

et al. 2016

Phil. Trans. R. Soc. A.

View full text Add to dashboard Cite

Quantitative analysis with mass spectrometry (MS) is important but challenging. Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) MS offers superior sensitivity, resolution and speed, but such techniques have numerous disadvantages that hinder quantitative analyses. This review summarizes essential obstacles to analyte quantification with MALDI-TOF MS, including the complex ionization mechanism of MALDI, sensitive characteristics of the applied electric fields and the mass-dependent detection efficiency of ion detectors. General quantitative ionization and desorption interpretations of ion production are described. Important instrument parameters and available methods of MALDI-TOF MS used for quantitative analysis are also reviewed.This article is part of the themed issue 'Quantitative mass spectrometry'.

show abstract