Models and simulations as enabling technologies for bioprinting process design

Conti, Michele; Santesarti, Gianluca; Scocozza, Franca; Marino, Michele

doi:10.1016/b978-0-323-85430-6.00009-1

Cited by 4 publications

(7 citation statements)

References 113 publications

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“…Finally, there is the bioprinting strategy, an additive manufacturing technique, which incorporates bioinks that are constituted of cells resuspended in biocompatible materials. 57…”

Section: Modeling the Lymphoid Microenvironment In 3d For Blood Cance...mentioning

confidence: 99%

“…Finally, there is the bioprinting strategy, an additive manufacturing technique, which incorporates bioinks that are constituted of cells resuspended in biocompatible materials. 57 Seeded scaffolds enable culture of cells in 3D without stressing them and giving them complete freedom to organize, but have 2 main limits: the technical variability produced by multiple seedings, and difficulty in obtaining a specific, customized, and precise architecture and compartmentalization. The 3D bioprinting has a great versatility and it allows the generation of multiple scaffolds with high levels of reproducibility, enabling precise control of scaffold composition, spatial distribution of cells and matrix, and specific architecture of the structure.…”

Section: Scaffold-based Modelsmentioning

confidence: 99%

“…The 3D bioprinting has a great versatility and it allows the generation of multiple scaffolds with high levels of reproducibility, enabling precise control of scaffold composition, spatial distribution of cells and matrix, and specific architecture of the structure. 57 In literature, there are multiple examples showing the potentialities of this technique. 58,59 Wu et al fabricated a model of MM BM niche through bioprinting.…”

Section: Scaffold-based Modelsmentioning

confidence: 99%

“…The key approach in this context could be a stepwise increase in the complexity of the 3D culture, which is also necessary for another delicate and important aspect—the standardization and reproducibility of the models 1 , 3 . The use of novel techniques such as 3D bioprinting and dynamic growth in bioreactors can be of great help 10 , 57 , 60 . The bioprinting technique is helpful in several aspects, in particular to reproducing the architectural complexity of lymphoid tissues, because it is possible to depose predefined structures with different compositions 57 .…”

Section: Challengesmentioning

confidence: 99%

“…Differently from scaffold‐free spheroids, those cultures remain mixed with the hydrogel, which can be used as a structural and functional support for their development. Finally, there is the bioprinting strategy, an additive manufacturing technique, which incorporates bioinks that are constituted of cells resuspended in biocompatible materials 57 …”

Section: Modeling the Lymphoid Microenvironment In 3d For Blood Cance...mentioning

confidence: 99%

See 4 more Smart Citations

Emerging Strategies in 3D Culture Models for Hematological Cancers

Barozzi

Scielzo

2023

HemaSphere

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In vitro cell cultures are fundamental and necessary tools in cancer research and personalized drug discovery. Currently, most cells are cultured using two-dimensional (2D) methods, and drug testing is mainly performed in animal models. However, new and improved methods that implement three-dimensional (3D) cell-culturing techniques provide compelling evidence that more advanced experiments can be performed, yielding valuable new insights. In 3D cell-culture experiments, the cell environment can be manipulated to mimic the complexity and dynamicity of the human tissue microenvironment, possibly leading to more accurate representations of cell-to-cell interactions, tumor biology, and predictions of drug response. The 3D cell cultures can also potentially provide alternative ways to study hematological cancers and are expected to eventually bridge the gap between 2D cell culture and animal models. The present review provides an overview of the complexity of the lymphoid microenvironment and a summary of the currently used 3D models that aim at recreating it for hematological cancer research. We here dissect the differences and challenges between, and potential advantages of, different culture methods and present our vision of the most promising future strategies in the hematological field.

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“…Finally, there is the bioprinting strategy, an additive manufacturing technique, which incorporates bioinks that are constituted of cells resuspended in biocompatible materials. 57…”

Section: Modeling the Lymphoid Microenvironment In 3d For Blood Cance...mentioning

confidence: 99%

Section: Scaffold-based Modelsmentioning

confidence: 99%

Section: Scaffold-based Modelsmentioning

confidence: 99%

Section: Challengesmentioning

confidence: 99%

Section: Modeling the Lymphoid Microenvironment In 3d For Blood Cance...mentioning

confidence: 99%

See 3 more Smart Citations

Emerging Strategies in 3D Culture Models for Hematological Cancers

Barozzi

Scielzo

2023

HemaSphere

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A Phase-field model of cell motility in biodegradable hydrogel scaffolds for tissue engineering applications

Gaziano,

Marino

2023

Comput Mech

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Computational modeling of cell motility and clusters formation in enzyme-sensitive hydrogels

Gaziano,

Marino

2024

Meccanica

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In this paper, we propose an extension of a previous model of cell motility in tissue engineering applications recently developed by the authors. Achieving large-scale production of neo-tissue through biofabrication technologies remains challenging owing to the need of thoroughly optimizing all the relevant process variables, a task hardly attainable through solely trial and error approaches. Therefore, the present work is intended to provide a valid and effective computational-based support for neo-tissue formation, with a specific focus on the preliminary phase of such process, in which cells move through a polymeric scaffold (hydrogel) and then compact into clusters. Cell motility is modeled by resorting to the phase-field method, and by incorporating diffusion of nutrients from the external culture bath as well as the expression by cells of chemoattractant substances that bias the random path they otherwise would follow. The previous model has been enriched by additionally encompassing the secretion of enzymes by cells that cleave the crosslinks between the hydrogel polymer chains. As such, in the present model hydrogel degradation exhibits spatio-temporal variations in its chemo-physical properties related to the local amount of enzymes, which deeply affects cell motility. Numerical results showcase the pivotal importance of the cells micro-environment properties for their crawling in hydrogel scaffolds, opening towards the development of a predictive computational-aided optimization tool for neo-tissue growth in bioprinted scaffolds.

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Models and simulations as enabling technologies for bioprinting process design

Cited by 4 publications

References 113 publications

Emerging Strategies in 3D Culture Models for Hematological Cancers

Emerging Strategies in 3D Culture Models for Hematological Cancers

A Phase-field model of cell motility in biodegradable hydrogel scaffolds for tissue engineering applications

Computational modeling of cell motility and clusters formation in enzyme-sensitive hydrogels

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