Modelling the relative contribution of seed nitrogen reserves and external nitrogen uptake during heterotrophic growth in Medicago truncatula

Brunel‐Muguet, Sophie; Mary, Bruno; Dürr, Carolyne

doi:10.1007/s11104-014-2182-x

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…Gaining knowledge on genetic variation and architecture of traits associated with seedling performance such as traits optimizing N acquisition (Kiba and Krapp 2016), seed biomass allocation to the seedling organs, or nitrogen use efficiency during this phase is a first step necessary for the identification and incorporation of new traits and alleles in breeding programs. Only few works, on small number of genotypes, studied allocation of seed biomass between seedling organs during heterotrophic growth (Dürr and Mary 1998, Brunel et al 2009, Brunel‐Muguet et al 2015). Phenotypic plasticity can also provide relevant adaptive traits in response to climatic variability or management actions (Wu et al 2011, Yu et al 2014, Casadebaig et al 2015, Mašková and Herben 2018, Scheepens et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Exploring natural diversity of Medicago truncatula reveals physiotypes and loci associated with the response of seedling performance to nitrate supply

Hdech

Aubry

Alibert

et al. 2020

Physiologia Plantarum

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Seedling pre‐emergence is a critical phase of development for successful crop establishment because of its susceptibility to environmental conditions. In a context of reduced use of inorganic fertilizers, the genetic bases of the response of seedlings to nitrate supply received little attention. This issue is important even in legumes where nitrate absorption starts early after germination, before nodule development. Natural variation of traits characterizing seedling growth in the absence or presence of nitrate was investigated in a core collection of 192 accessions of Medicago truncatula. Plasticity indexes to the absence of nitrate were calculated. The genetic determinism of the traits was dissected by genome‐wide association study (GWAS). The absence of nitrate affected seed biomass mobilization and root/shoot length ratio. However, the large range of genetic variability revealed different seedling performances within natural diversity. A principal component analysis (PCA) carried out with plasticity indexes highlighted four physiotypes of accessions differing in relationships between seedling elongation and seed biomass partitioning traits in response to the absence of nitrate. Finally, GWAS revealed 45 associations with single or combined traits corresponding to coordinates of accessions on PCA, as well as two clusters of genes encoding sugar transporters and glutathione transferases surrounding loci associated with seedling elongation traits.

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Section: Introductionmentioning

confidence: 99%

Exploring natural diversity of Medicago truncatula reveals physiotypes and loci associated with the response of seedling performance to nitrate supply

Hdech

Aubry

Alibert

et al. 2020

Physiologia Plantarum

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“…After sprouting, the radicle protrudes from the seed coat to explore the soil for nutrients (Benoit et al 2015, Durr and Mary 1998, Li et al 2019. Radicle growth depends on both seed mineral reserves and nutrient uptake from the substrate (Brunel-Muguet et al 2015). This is especially true in small seeds (Peñaloza and Duran, 2015), such as ryegrass.…”

Section: Resultsmentioning

confidence: 99%

Metal phytotoxicity thresholds in copper smelter-contaminated soils

Zhikharev

Sahakyan

Tepanosyan

et al. 2022

Idesia

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Environmental problems associated with non-ferrous smelter operations are a problem in many countries around the world. Copper production in Armenia has resulted in metal contamination of soils and general degradation of environmental quality. In this study we attempted to determine the thresholds of phytotoxicity of metals in soils contaminated by a copper smelter in Armenia. The studied soils were sampled in the Alaverdi valley, located ~200 km north of Yerevan. The range of metal concentrations in the studied soils was very wide, e.g., total copper in the soil was 87-5885 mg kg -1 . Most soils had nearly neutral pH (6.9 ± 0.57), and average organic matter content (6.3 ± 1.9). Some soils were saline. Perennial ryegrass root elongation was used as an operational method to assess phytotoxicity. We calculated a contamination index for each soil studied and plotted root length as a function of soil contamination index. Root growth was mainly affected by the contamination index based on total metal concentration, while the contamination index based on extractable metal concentration proved to be a weak predictor. Soil organic matter had a stimulatory (positive) effect, whereas electrical conductivity had a toxic (negative) effect on root growth. Despite these confounding factors, we were able to derive phytotoxicity thresholds for metals (EC 10 , EC 25 , EC 50 ) based on the contamination index. In conclusion, we discuss the practical importance of this study and future research needs concerning contaminated soils in the Alaverdi Valley.

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“…In Brassicaceae species, radicle protrusion occurs approximately 2 days after sowing (Dell'Aquila et al 2000;Russo et al 2010). Radicle growth depends on both mineral seed reserves and nutrient uptake from the substrate (Brunel-Muguet et al 2015). This is especially true in small seeds (Peñaloza and Durán 2015), such as mustard seeds.…”

Section: Differences In Root Elongation Results Under the Two Protocolsmentioning

confidence: 99%

Root Elongation Method for the Quality Assessment of Metal-Polluted Soils: Whole Soil or Soil-Water Extract?

Prudnikova

Neaman

Терехова

et al. 2020

J Soil Sci Plant Nutr

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Root elongation method may be implemented using two internationally accepted protocols: exposing plants to either soil-water extract or whole soil. But which of the two protocols is more suitable for root elongation analysis undertaken for the quality assessment of metal-polluted soils? Soils were sampled at various distances from the site of the Middle Urals Copper Smelter located in Russia. White mustard was used as a bioindicator. We observed considerable differences in root elongation under the two protocols. In plants grown in whole soil, root length inversely correlated with pollution index, but in soil-water extract, metal concentrations had no effect on root length. Nutrient and metal concentrations in the soil-water extract were not buffered, due to the absence of the solid soil phase. It is for this reason that in highly polluted soils, root growth was greater in soil-water extracts rather than in whole soils, whereas in background soils (in the absence of toxicity), root growth was greater in whole soils compared with soil-water extracts. The quantity, intensity, and capacity factors are a plausible explanation for the differences in root length between the two protocols. The soil-water extract does not represent actual soil with respect to the desorptiondissolution reactions that take place between the soil solid phase and the soil solution. For this reason, whole soil protocol should be used for measuring root elongation given that only under this protocol, direct contact between metal-polluted soil and test organisms correctly replicates the risks inherent in the actual soil habitat.

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Modelling the relative contribution of seed nitrogen reserves and external nitrogen uptake during heterotrophic growth in Medicago truncatula

Cited by 5 publications

References 22 publications

Exploring natural diversity of Medicago truncatula reveals physiotypes and loci associated with the response of seedling performance to nitrate supply

Exploring natural diversity of Medicago truncatula reveals physiotypes and loci associated with the response of seedling performance to nitrate supply

Metal phytotoxicity thresholds in copper smelter-contaminated soils

Root Elongation Method for the Quality Assessment of Metal-Polluted Soils: Whole Soil or Soil-Water Extract?

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