Modelling the reaction mechanism of ribulose‐1,5‐bisphosphate carboxylase/oxygenase and consequences for kinetic parameters

Tcherkez, Guillaume

doi:10.1111/pce.12066

Cited by 69 publications

(97 citation statements)

References 65 publications

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“…The reasons for this are unclear. Furthermore, the interpretation of the E a for K c is not straightforward because there is no Michaelis-Menten complex between Rubisco and CO 2 (Tcherkez 2013). Hochachka and Somero (2002) describe a similar covariation in the k cat and K M of lactate dehydrogenase (LDH) in fish from differing thermal habitat.…”

Section: Discussionmentioning

confidence: 99%

Temperature dependence of in vitro Rubisco kinetics in species of Flaveria with different photosynthetic mechanisms

et al. 2015

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There is general consensus in the literature that plants with different photosynthetic mechanisms (i.e. C3 vs. C4) have Rubiscos characterised by different kinetic performances. However, potential differences in the temperature dependencies of Rubisco kinetic parameters between C3 and C4 plants are uncertain. Accordingly, six species of Flaveria with contrasting photosynthetic mechanisms (C3, C3/C4 and C4) were selected and their Rubisco Michaelis-Menten constants for CO2 and RuBP (K c and K RuBP), carboxylase catalytic turnover rate ([Formula: see text]) and CO2/O2 specificity factor (S c/o) were measured between 10 and 40 °C. The results confirmed different Rubisco characteristics between C3 and C4 plants. Rubisco from the C3 species had higher E a for K c and [Formula: see text] than that from C4 species, which were translated into differences in the temperature response of the carboxylase catalytic efficiency ([Formula: see text]/K c). However, E a did not differ for S c/o or K RuBP. Although a mechanism remains uncertain, it appears that the Asp/Glu-149-Ala and Met-309-Ile substitutions lead to differences in the temperature responses of catalysis between C3 and C4 Rubiscos in Flaveria. Therefore, the above observations are consistent with the fact that C3 species have a higher photosynthetic efficiency and ecological dominance in cool environments, with respect to C4 species in temperate environments.

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Section: Discussionmentioning

confidence: 99%

Temperature dependence of in vitro Rubisco kinetics in species of Flaveria with different photosynthetic mechanisms

et al. 2015

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“…To combat photorespiration, it appears that Rubisco in plants has evolved to become more specific for CO 2 , but achieving specificity in evolution appears to have been at the expense of speed of catalysis. Indeed, the catalytic rate of Rubisco from plants is one of the slowest of any enzyme-catalyzed reactions at $3.7 per active site per second (Parry et al, 2013;Tcherkez, 2013;Zhu et al, 2004b). Modern forms of Rubisco therefore represent a compromise between specificity (t) and catalytic rate (k cat ).…”

Section: Targets For Increasing Crop Photosynthetic Efficiencymentioning

confidence: 99%

Meeting the Global Food Demand of the Future by Engineering Crop Photosynthesis and Yield Potential

Long

Marshall‐Colón

Zhu

2015

Cell

823

580

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Increase in demand for our primary foodstuffs is outstripping increase in yields, an expanding gap that indicates large potential food shortages by mid-century. This comes at a time when yield improvements are slowing or stagnating as the approaches of the Green Revolution reach their biological limits. Photosynthesis, which has been improved little in crops and falls far short of its biological limit, emerges as the key remaining route to increase the genetic yield potential of our major crops. Thus, there is a timely need to accelerate our understanding of the photosynthetic process in crops to allow informed and guided improvements via in-silico-assisted genetic engineering. Potential and emerging approaches to improving crop photosynthetic efficiency are discussed, and the new tools needed to realize these changes are presented.

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“…In low irradiance, 30-277 50% of the total pool of Rubisco is active (Pearcy, 1988;Lan et al, 1992;Carmo-Silva 278 and Salvucci, 2013). The remainder is activated with a τ of 3-5 minutes after switching 279 to high irradiance (Pearcy, 1988;Woodrow and Mott, 1989;Kaiser et al, 2016;Taylor 280 and Long, 2017 (Tcherkez, 2013). Rubisco activates more quickly at higher CO 2 partial pressures, 284 both in folio (Kaiser et al, 2017) and in vitro (Woodrow et al, 1996), a phenomenon that 285…”

Section: Hangartermentioning

confidence: 99%

Fluctuating Light Takes Crop Photosynthesis on a Rollercoaster Ride

2017

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Modelling the reaction mechanism of ribulose‐1,5‐bisphosphate carboxylase/oxygenase and consequences for kinetic parameters

Abstract: ABSTRACT

Cited by 69 publications

References 65 publications

Temperature dependence of in vitro Rubisco kinetics in species of Flaveria with different photosynthetic mechanisms

Temperature dependence of in vitro Rubisco kinetics in species of Flaveria with different photosynthetic mechanisms

Meeting the Global Food Demand of the Future by Engineering Crop Photosynthesis and Yield Potential

Fluctuating Light Takes Crop Photosynthesis on a Rollercoaster Ride

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