Modelling the effect of pH and water activity in the growth of<i>Aspergillus fumigatus</i>isolated from corn silage

Alonso, Veronica Andrea; Cavaglieri, Lilia Reneé; Ramos, Ana M. González; Torres, Adriana Mabel; Marı́n, Sonia

doi:10.1111/jam.13395

Cited by 21 publications

(15 citation statements)

References 40 publications

(66 reference statements)

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“…The GIT organs (rumen, abomasum, jejunum, cecum and colon) were opened and the digesta was mixed and collected into tubes: rumen (8 tubes of 50 mL), abomasums (4 tubes of 15 mL), jejunum (4 tubes of 15 mL), cecum (4 tubes of 15 mL) and colon (4 tubes of 15 mL). Ruminal sample pH was measured (PHS-100 portable acidity meter, Tianqi Mdt InfoTech, Ltd., ShangHai, China) [13]; Rumen samples were filtered through four layers of cheesecloth into 3 tubes. Subsequently, this rumen fluid (approximately 20 mL) was mixed with 5mL solution of 25% metaphosphoric acid and preserved at -20˚C for…”

Section: Animals and Sample Collectionmentioning

confidence: 99%

Relationship between true digestibility of dietary phosphorus and gastrointestinal bacteria of goats

et al. 2020

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The present research was conducted to evaluate the connection between the true digestibility of Phosphorus (TDP) in diet and bacterial community structure in the gastrointestinal tract (GIT) of goats. Twenty-eight Nubian goats were chosen and metabolic experiment was conducted to analyze TDP of research animals. Eight goats were grouped into the high digestibility of phosphorus (HP) phenotype, and another 8 were grouped into the low digestibility of phosphorus (LP) phenotype. And from the rumen, abomasum, jejunim, cecum and colon content of the goats, bacterial 16S rRNA gene amplicons were sequenced. In the rumen 239 genera belonging to 23 phyla, in abomasum 319 genera belonging to 30 phyla, in jejunum 248 genera belonging to 36 phyla, in colon 248 genera belonging to 25 phyla and in cecum 246 genera belonging to 23 phyla were noticed. In addition, there was a significant correlation between the TDP and the abundance of Ruminococcaceae_UCG-010, Rumino-coccus_2, Ruminococcaceae_UCG-014, Selenomonas_1 and Prevotella in the rumen, Lachnospiraceae_ND3007_group, Saccharofermentans, Ruminococcus_1, Ruminococca-ceae_UCG-014, Lachnospiraceae_XPB1014_group and Desulfovibrio in the abomasum, Prevotella, Clostridium_sensu_stricto_1, Fibrobacter, Desulfovibrio and Ruminococcus_2 in the jejunum, Ruminococcaceae_UCG-014 in the colon, and Desulfovibrio in the cecum. Present research trial recommended that the community of gastrointestinal microbiota is a factor affecting TDP in goats.

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Section: Animals and Sample Collectionmentioning

confidence: 99%

Relationship between true digestibility of dietary phosphorus and gastrointestinal bacteria of goats

et al. 2020

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“…A general aspect in the growth kinetics of individual hyphae is the exponential hyphal extension at a constant specific rate in the early growth phase 2 . The Baranyi model 52 used for the analysis of the germination data is a lag-exponential growth model, originally introduced for modelling bacterial growth in food but also valid for modelling growth in fungi 4 . This behaviour is described in detail by Gougouli et al .…”

Section: Discussionmentioning

confidence: 99%

HyphaTracker: An ImageJ toolbox for time-resolved analysis of spore germination in filamentous fungi

Brunk

Sputh

Doose

et al. 2018

Sci Rep

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The dynamics of early fungal development and its interference with physiological signals and environmental factors is yet poorly understood. Especially computational analysis tools for the evaluation of the process of early spore germination and germ tube formation are still lacking. For the time-resolved analysis of conidia germination of the filamentous ascomycete Fusarium fujikuroi we developed a straightforward toolbox implemented in ImageJ. It allows for processing of microscopic acquisitions (movies) of conidial germination starting with drift correction and data reduction prior to germling analysis. From the image time series germling related region of interests (ROIs) are extracted, which are analysed for their area, circularity, and timing. ROIs originating from germlings crossing other hyphae or the image boundaries are omitted during analysis. Each conidium/hypha is identified and related to its origin, thus allowing subsequent categorization. The efficiency of HyphaTracker was proofed and the accuracy was tested on simulated germlings at different signal-to-noise ratios. Bright-field microscopic images of conidial germination of rhodopsin-deficient F. fujikuroi mutants and their respective control strains were analysed with HyphaTracker. Consistent with our observation in earlier studies the CarO deficient mutant germinated earlier and grew faster than other, CarO expressing strains.

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“…Then the goats were slaughtered and the GIT organs (rumen, abomasum, jeujinu, cecum and colon) were unglued to collect rumen (8 tubes, each 50 mL), abomasums (4 tubes, each 15 mL), jejunum (4 tubes, each 15 mL), cecum (4 tubes, each 15 mL) and colon (4 tubes, each 15 mL) content. A tube of ruminal sample was subjected to measure the pH (PHS-100 portable acidity meter, Tianqi Mdt InfoTech, Ltd., ShangHai, China) [13]; a four layers of cheesecloth was used to filter the three tubes and then the fluid was collected. Subsequently, this rumen fluid (approximately 20 mL) was mixed with 5mL solution of meta-phosphoric acid and preserved at −20°C for further calculation of the volatile fatty acid concentration (VFA; acetate, propionate, and butyrate).…”

Section: Methodsmentioning

confidence: 99%

Relationship between True Digestibility of dietary Phosphorus and Gastrointestinal Bacteria of Goats

Wang

Shah

Liu

et al. 2019

Preprint

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22The present research was conducted to evaluate the connection between the true digestibility 23 of Phosphorus (TDP) in diet and bacterial community structure in the gastrointestinal tract (GIT) 24 of goats. Twenty-eight Nubian goats were chosen and metabolic experiment was conducted to 25 analyze TDP of research animals. Eight goats were grouped into the high digestibility of 26 phosphorus (HP) phenotype, and another 8 were grouped into the low digestibility of phosphorus 27 (LP) phenotype. And from the rumen, abomasum, jejunim, cecum and colon content of the goats, 28 bacterial 16S rRNA gene amplicons were sequenced. In the rumen 239 genera belonging to 23 29 phyla, in abomasum 319 genera belonging to 30 phyla, in jejunum 248 genera belonging to 36,in 30 colon 248 genera belonging to 25 phyla and in cecum 246 genera belonging to 23 phyla were 31 noticed. In addition, there was a significant correlation between the TDP and the abundance of 32 Ruminococcus_2, Selenomonas_1 33 and Prevotella in the rumen, Lachnospiraceae_ND3007_group, Saccharofermentans, 34 Ruminococcus_1, Ruminococcaceae_UCG-014, Lachnospiraceae_XPB1014_group and 35 Desulfovibrio in the abomasum, Prevotella, Clostridium_sensu_stricto_1, Fibrobacter, 36 Desulfovibrio and Ruminococcus_2 in the jejunum, Ruminococcaceae_UCG-014 in the colon, 37 and Desulfovibrio in the cecum. Present research trial recommended that the community of 38 gastrointestinal microbiota is a factor affecting TDP in goats. 3 39 Introduction 42 Phosphorus is a major mineral element that affects the growth and development of animals 43 and acts a vital biological part in the formation and growth of skeletons. Young animals develop 44 rickets, and adult animals present osteomalacia when animals lack phosphorus. In addition, 45 phosphorus also participates in the digestion and metabolism of almost all nutrients, such as 46 amino acids, lipids and carbohydrates, in animals in the form of nucleic acids, phosphoproteins 47 and phospholipids [1]. It was found that phosphorus in plant feedstuff is poorly or not at all 48 utilized by monogastric animals due to phytic acid (myo-inositol hexaphosphoric acid) binding. 49 The undigested phytate phosphorus is excreted with feces, which causes severe environmental 50 pollution problems, paying to surface water eutrophication in parts where the livestock 51 production of monogastric is intensive [2, 3] . Phytase is a type of phosphatase enzyme that 52 catalyzes the hydrolysis of phytic acid found in grains and oil seeds to myo-inositol and 53 inorganic phosphate, which are then absorbed in the small intestine [4]. Many previous 54 experiments have proven that supplementation of monogastric diets with phytase not only 55 significantly increased the bioavailability of phytic acid-bound phosphate and reduced 56 phosphorus excretion but also diminished the antinutritional effects of phytate [5, 6]. Almost all 57 of the phytases currently used in animal production are produced by microorganisms [7]. The 58 ruminant gastroint...

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Modelling the effect of pH and water activity in the growth ofAspergillus fumigatusisolated from corn silage

Cited by 21 publications

References 40 publications

Relationship between true digestibility of dietary phosphorus and gastrointestinal bacteria of goats

Relationship between true digestibility of dietary phosphorus and gastrointestinal bacteria of goats

HyphaTracker: An ImageJ toolbox for time-resolved analysis of spore germination in filamentous fungi

Relationship between True Digestibility of dietary Phosphorus and Gastrointestinal Bacteria of Goats

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