Modelling the dynamics of vesicle reshaping and scission under osmotic shocks

Campos, Christian Vanhille; Šarić, Anđela

doi:10.1039/d0sm02012e

Cited by 21 publications

(19 citation statements)

References 39 publications

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“…Upon osmotic reversal, the deformed vesicles exhibited reproducible formation of "daughter vesicles" through an endocytosis-like process in order to quickly incorporate the external medium, indicating that a sizeable bending energy (> 10 3 times the thermal energy) is stored in the deformed vesicles. These experimental results are broadly in line with previous experimental and computational studies on more complex, multicomponent vesicle systems, where both osmotic deformation and daughter vesicle formation have been observed [8][9][10][11][12]. Thus, even though neither of these studies were directly focussed on quantitatively determining the deformation threshold, the overall picture is that GUVs exhibit a significant tolerance to osmotic gradients before becoming visibly deformed from spherical shape.…”

Section: Introductionsupporting

confidence: 88%

Thermal fluctuations and osmotic stability of lipid vesicles

Wennerström,

Sparr,

Stenhammar

2022

Preprint

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Section: Introductionsupporting

confidence: 88%

Thermal fluctuations and osmotic stability of lipid vesicles

Wennerström,

Sparr,

Stenhammar

2022

Preprint

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“…2 A and B ). The time evolution of the filament tension and the tension transferred into the membrane curvature ( 24 ) is shown in Fig. 2 C for each case.…”

Section: Resultsmentioning

confidence: 99%

Physical mechanisms of ESCRT-III–driven cell division

Harker-Kirschneck

Hafner

Yao

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Living systems propagate by undergoing rounds of cell growth and division. Cell division is at heart a physical process that requires mechanical forces, usually exerted by assemblies of cytoskeletal polymers. Here we developed a physical model for the ESCRT-III–mediated division of archaeal cells, which despite their structural simplicity share machinery and evolutionary origins with eukaryotes. By comparing the dynamics of simulations with data collected from live cell imaging experiments, we propose that this branch of life uses a previously unidentified division mechanism. Active changes in the curvature of elastic cytoskeletal filaments can lead to filament perversions and supercoiling, to drive ring constriction and deform the overlying membrane. Abscission is then completed following filament disassembly. The model was also used to explore how different adenosine triphosphate (ATP)-driven processes that govern the way the structure of the filament is changed likely impact the robustness and symmetry of the resulting division. Comparisons between midcell constriction dynamics in simulations and experiments reveal a good agreement with the process when changes in curvature are implemented at random positions along the filament, supporting this as a possible mechanism of ESCRT-III–dependent division in this system. Beyond archaea, this study pinpoints a general mechanism of cytokinesis based on dynamic coupling between a coiling filament and the membrane.

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“…4e ). This suggests that the enveloping membrane of the superstructure protects the colony from the initial osmotic shock by acting as a barrier, and reshapes itself in response 44 , possibly also relieving membrane tension by drawing additional lipid material from the attached multilamellar reservoir. It was shown recently that hypertonic conditions cause the compression of magnetically-assembled vesicle clusters and decrease the permeability into the cluster 12 .…”

Section: Resultsmentioning

confidence: 99%

Colony-like Protocell Superstructures

Spustová

Katke

Villalmanzo

et al. 2021

Preprint

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We report the formation, growth, and dynamics of model protocell superstructures on solid surfaces, resembling single cell colonies. These structures, consisting of several layers of lipidic compartments enveloped in a dome-shaped outer lipid bilayer, emerged as a result of spontaneous shape transformation of lipid agglomerates deposited on thin film aluminum surfaces. Collective protocell structures were observed to be mechanically more stable compared to isolated spherical compartments. We show that the model colonies encapsulate DNA and accommodate non-enzymatic, strand displacement DNA reactions. The membrane envelope is able to disassemble and expose individual daughter protocells, which can migrate and attach via nano-tethers to distant surface locations, while maintaining their encapsulated contents. Some colonies feature 'exo-compartments', which spontaneously extend out of the enveloping bilayer, internalize DNA, and merge again with the superstructure. A continuum elastohydrodynamic theory that we developed reveals that the subcompartment formation must be governed by attractive van der Waals (vdW) interactions between the membrane and surface. The balance between membrane bending and vdW interactions yields a critical length scale of 273 nm, above which the membrane invaginations can form subcompartments. The findings support our hypotheses that in extension of the 'lipid world hypothesis', protocells may have existed in the form of colonies, potentially benefiting from the increased mechanical stability provided by a superstructure.

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Modelling the dynamics of vesicle reshaping and scission under osmotic shocks

Abstract: We study the effects of osmotic shocks on lipid vesicles via coarse-grained molecular dynamics simulations by explicitly considering the solute in the system. We find that depending on their nature...

Cited by 21 publications

References 39 publications

Thermal fluctuations and osmotic stability of lipid vesicles

Thermal fluctuations and osmotic stability of lipid vesicles

Physical mechanisms of ESCRT-III–driven cell division

Colony-like Protocell Superstructures

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