A lot of different chemical reactions take place in the biochemical process of biogas formation but the most important of them include the reaction of bonding carbon dioxide with hydrogen and the decomposition of acetic acid. Other factors, such as temperature, pH, etc., only limit the amount of methane or, in extreme cases, they even stop the process of methane formation. The paper presents an analysis of the influence of the amount of available carbon in the substrate and inoculum on biogas production, as well as of the validity of the relation between methane production and carbon/hydrogen ratio which is often mentioned in the literature. The analyses were made on the basis of the results of several dozen laboratory experiments on methane production for five groups of substrates: cultivated plants, animal faeces, plant waste, animal waste and municipal waste. This provided the basis for the formulation of the conclusion that there is no significant relation between the carbon/hydrogen ratio and methane production, and an alternative biogas calculator was suggested to estimate methane production with the known content of carbon in the substrate and inoculum. This calculator was also adapted to the conditions of agricultural biogas plants, and then it was tested in those conditions. It should also be mentioned that the innovative aspect of the study presented herein is the model developed for the estimation of methane production on the basis of carbon content only, providing estimates with a smaller error than in the case of the calculators!