Modelling of multi-nutrient interactions in growth of the dinoflagellate microalga Protoceratium reticulatum using artificial neural networks

López-Rosales, L.; Gallardo, Juan José; Sánchez-Mirón, A.; Contreras-Gómez, Antonio; Camacho, F. Garcı́a; Grima, E. Molina

doi:10.1016/j.biortech.2013.07.141

Cited by 23 publications

(11 citation statements)

References 25 publications

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“…A genetic-algorithm-based approach has been previously shown to be quite successful for formulating a culture medium of the dinoflagellate Protoceratium reticulatum [8,11]. Genetic algorithm (GA) based methods are superior to the conventional statistical experimental designs that have been commonly used for developing microbial culture media.…”

Section: Introductionmentioning

confidence: 99%

An optimal culture medium for growing Karlodinium veneficum : Progress towards a microalgal dinoflagellate-based bioprocess

et al. 2015

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Section: Introductionmentioning

confidence: 99%

An optimal culture medium for growing Karlodinium veneficum : Progress towards a microalgal dinoflagellate-based bioprocess

et al. 2015

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“…Aseptic operation was used as production of KTXs for medical investigational purposes requires the use of a well-defined pure culture. As toxin productivity and growth of diverse dinoflagellates are strongly influenced by nutrition (Dagenais-Bellefeuille and Morse, 2013; López-Rosales et al, 2013), a culture medium specifically developed for production of KTXs by K. veneficum (López-Rosales et al, 2015b) was used. The culture systems were designed and compared using the energy dissipation rate fields obtained by computational fluid dynamics (CFD) simulations.…”

Section: Introductionmentioning

confidence: 99%

Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract

López-Rosales

Sánchez-Mirón

Camacho

et al. 2018

Bioresource Technology

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A pilot-scale bioprocess was developed for the production of karlotoxin-enriched extracts of the marine algal dinoflagellate Karlodinium veneficum. A bubble column and a flat-panel photobioreactors (80-281 L) were used for comparative assessment of growth. Flow hydrodynamics and energy dissipation rates (EDR) in the bioreactors were characterized through robust computational fluid dynamic simulations. All cultures were conducted monoseptically outdoors. Bubble column (maximum cell productivity in semicontinuous operation of 58 × 10 cell mL day) proved to be a better culture system for this alga. In both reactors, the local EDR near the headspace, and in the sparger zone, were more than one order of magnitude higher than the average value in the whole reactor (=4 × 10 W kg). Extraction of the culture and further purification resulted in the desired KTXs extracts. Apparently, the alga produced three congeners KTXs: KmTx-10 and its sulfated derivative (sulfo-KmTx-10) and KmTx-12. All congeners possessed hemolytic activity.

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“…Field and culture-based studies with HAB dinoflagellates support the idea that exogenous B vitamins (i.e., B 1 , B 7 , B 12 ) have the potential to broadly influence marine biomass productivity and associated composition [ 313 ]. Tests with P. reticulatum and K. veneficum , using artificial neural networks as predictive tool for nonlinear interactions among all nutrients in culture media, suggest that micronutrients and vitamins (even to lower concentrations) are relatively more significant than macronutrients toward growth of both microalgae [ 314 , 315 ].…”

Section: Biotoxin and Bioactive Productionmentioning

confidence: 99%

Biotechnological and Pharmacological Applications of Biotoxins and Other Bioactive Molecules from Dinoflagellates

2017

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Abstract:The long-lasting interest in bioactive molecules (namely toxins) produced by (microalga) dinoflagellates has risen in recent years. Exhibiting wide diversity and complexity, said compounds are well-recognized for their biological features, with great potential for use as pharmaceutical therapies and biological research probes. Unfortunately, provision of those compounds is still far from sufficient, especially in view of an increasing demand for preclinical testing. Despite the difficulties to establish dinoflagellate cultures and obtain reasonable productivities of such compounds, intensive research has permitted a number of advances in the field. This paper accordingly reviews the characteristics of some of the most important biotoxins (and other bioactive substances) produced by dinoflagellates. It also presents and discusses (to some length) the main advances pertaining to dinoflagellate production, from bench to large scale-with an emphasis on material published since the latest review available on the subject. Such advances encompass improvements in nutrient formulation and light supply as major operational conditions; they have permitted adaptation of classical designs, and aided the development of novel configurations for dinoflagellate growth-even though shearing-related issues remain a major challenge.

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Modelling of multi-nutrient interactions in growth of the dinoflagellate microalga Protoceratium reticulatum using artificial neural networks

Cited by 23 publications

References 25 publications

An optimal culture medium for growing Karlodinium veneficum : Progress towards a microalgal dinoflagellate-based bioprocess

An optimal culture medium for growing Karlodinium veneficum : Progress towards a microalgal dinoflagellate-based bioprocess

Pilot-scale outdoor photobioreactor culture of the marine dinoflagellate Karlodinium veneficum: Production of a karlotoxins-rich extract

Biotechnological and Pharmacological Applications of Biotoxins and Other Bioactive Molecules from Dinoflagellates

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