Modelling of multi-conjugate adaptive optics for spatially variant aberrations in microscopy

Simmonds, Richard; Booth, Martin J.

doi:10.1088/2040-8978/15/9/094010

Cited by 43 publications

(32 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…43 Another consequence of deeper focusing is the increase in scattering. The methods used so far in adaptive microscopes have provided only a fixed aberration correction for each image, but it is known that the aberrations can change across the field of view.…”

Section: Resultsmentioning

confidence: 99%

Adaptive Optics for Fluorescence Microscopy

Booth¹,

Patton²

2014

Fluorescence Microscopy

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Adaptive Optics for Fluorescence Microscopy

Booth¹,

Patton²

2014

Fluorescence Microscopy

View full text Add to dashboard Cite

“…More recently, this same principle of conjugate AO has made its way to the microscopy community. Reports in both simulation [6][7][8] and experiment [9][10][11][12][13] have demonstrated the FOV advantage of conjugate AO as well as its feasibility. For example, conjugate AO has been applied in scanning microscopy configurations using both one- 9 and twophoton 10,11 microscopy.…”

Section: Introductionmentioning

confidence: 93%

Widefield fluorescence microscopy with sensor-based conjugate adaptive optics using oblique back illumination

Bifano

Mertz

2016

J. Biomed. Opt.

View full text Add to dashboard Cite

fluorescence microscopy with sensor-based conjugate adaptive optics using oblique back illumination," J. Abstract. We describe a wavefront sensor strategy for the implementation of adaptive optics (AO) in microscope applications involving thick, scattering media. The strategy is based on the exploitation of multiple scattering to provide oblique back illumination of the wavefront-sensor focal plane, enabling a simple and direct measurement of the flux-density tilt angles caused by aberrations at this plane. Advantages of the sensor are that it provides a large measurement field of view (FOV) while requiring no guide star, making it particularly adapted to a type of AO called conjugate AO, which provides a large correction FOV in cases when sample-induced aberrations arise from a single dominant plane (e.g., the sample surface). We apply conjugate AO here to widefield (i.e., nonscanning) fluorescence microscopy for the first time and demonstrate dynamic wavefront correction in a closed-loop implementation.

show abstract

“…Simulations have shown the potential benefit of this approach, although practical implementations are still to be seen. 21,22 ADAPTIVE OPTICS METHODS FOR MICROSCOPY Recent progress in AO microscopy has been enabled by two main factors. The first factor is the commercial availability of compact deformable mirrors and spatial light modulators as adaptive correction elements.…”

Section: Applications Of Adaptive Microscopymentioning

confidence: 99%

Adaptive optical microscopy: the ongoing quest for a perfect image

2014

Self Cite

View full text Add to dashboard Cite

Adaptive optics is becoming a valuable tool for high resolution microscopy, providing correction for aberrations introduced by the refractive index structure of specimens. This is proving particularly promising for applications that require images from deep within biological tissue specimens. We review recent developments in adaptive microscopy, including methods and applications. A range of advances in different microscope modalities is covered and prospects for the future are discussed. INTRODUCTIONOptical microscopes have for a long time played a vital role in biomedical research. Their ability to provide multidimensional structural and functional information about a specimen in a non-invasive manner has permitted numerous scientific advances. These microscopes rely upon high-quality optical systems to operate at the optimum resolution, which is determined by the diffraction limit of light for conventional microscopes. However, even with perfect optical components, the performance of the microscope is affected by the optical properties of the specimen. Spatial variations in refractive index introduce aberrations as the light passes through the specimen-a problem that is exacerbated when imaging deeps into tissue. These aberrations detrimentally affect the resolution and contrast of microscope images, ultimately limiting the depth at which imaging is practical. Adaptive optics (AO) was introduced into microscopy in order to overcome this limitation. Dynamic correction elements, such as deformable mirrors (DMs) or spatial light modulators (SLMs) have been employed to compensate specimen-induced aberrations (Figure 1). New wavefront sensing and control schemes have been developed to adapt the correction elements.The first demonstrations of adaptive optical microscopy took place in the early 2000s. In the few years that followed, there were several innovative advances that set the scene for further research. The research in this period is well covered by two review articles 1,2 and a recent compiled volume, 3 so this will not be reproduced here. This present article provides a review of advances in aberration correction in adaptive microscopy that have taken place in the last half-decade. During this period, a wide range of AO techniques have emerged, which have considerably enhanced the available toolkit. Furthermore, the benefit of correction of specimen-induced aberrations has been shown in different areas, particularly (although not exclusively) in biomedical applications. We outline these technological

show abstract

Modelling of multi-conjugate adaptive optics for spatially variant aberrations in microscopy

Cited by 43 publications

References 16 publications

Adaptive Optics for Fluorescence Microscopy

Adaptive Optics for Fluorescence Microscopy

Widefield fluorescence microscopy with sensor-based conjugate adaptive optics using oblique back illumination

Adaptive optical microscopy: the ongoing quest for a perfect image

Contact Info

Product

Resources

About