Modelling nutrient transport in hollow fibre membrane bioreactors for growing three-dimensional bone tissue

Ye, Hua; Das, Diganta Bhusan; Triffitt, James T.; Cui, Zhanfeng

doi:10.1016/j.memsci.2005.07.040

Cited by 74 publications

(72 citation statements)

References 24 publications

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“…This includes, but is not limited to micro fluidic systems, 2, 3 nutrient transport in bloodflow, 4,5 single and multiphase transport in porous media, [6][7][8][9][10][11][12] and transport in groundwater systems. [13][14][15][16] The basic idea behind Taylor dispersion is simple.…”

Section: Introductionmentioning

confidence: 99%

The impact of inertial effects on solute dispersion in a channel with periodically varying aperture

et al. 2012

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We investigate solute transport in channels with a periodically varying aperture, when the flow is still laminar but sufficiently fast for inertial effects to be nonnegligible. The flow field is computed for a two-dimensional setup using a finite element analysis, while transport is modeled using a random walk particle tracking method. Recirculation zones are observed when the aspect ratio of the unit cell and the relative aperture fluctuations are sufficiently large; under non-Stokes flow conditions, the flow in non-reversible, which is clearly noticeable by the horizontal asymmetry in the recirculation zones. After characterizing the size and position of the recirculation zones as a function of the geometry and Reynolds number, we investigate the corresponding behavior of the longitudinal effective diffusion coefficient. We characterize its dependence on the molecular diffusion coefficient D m , the Péclet number, the Reynolds number, and the geometry. The proposed relation is a generalization of the well-known Taylor-Aris relationship relating the longitudinal dispersion coefficient to D m and the Péclet number for a channel of constant aperture at sufficiently low Reynolds number. Inertial effects impact the exponent of the Péclet number in this relationship; the exponent is controlled by the relative amplitude of aperture fluctuations. For the range of parameters investigated, the measured dispersion coefficient always exceeds that corresponding to the parallel plate geometry under Stokes conditions; in other words, boundary fluctuations always result in increased dispersion. The transient approach to the asymptotic regime is also studied and characterized quantitatively. We show that the measured characteristic time to attain asymptotic conditions is controlled by two competing effects: (i) the trapping of particles in the near-immobile zone and, (ii) the enhanced mixing in the central zone where most of the flow takes place (mainstream), due to its thinning. C 2012 American Institute of Physics. [http://dx

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Section: Introductionmentioning

confidence: 99%

The impact of inertial effects on solute dispersion in a channel with periodically varying aperture

et al. 2012

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“…To overcome nutrient diffusion limitations, biological angiogenesis is the primary requirement to generate dense tissues. However, angiogenesis remains a technical challenge in tissue engineering (9). Within this context, HFBs were designed to mimic the native blood capillary system in the aim to enhance the molecule transport and mass exchange between the fluid (i.e., medium) and the cell phase by introducing a velocity flow field (i.e., convective term).…”

Section: Three-dimensional Tissuesmentioning

confidence: 99%

Hollow Fiber Bioreactor Technology for Tissue Engineering Applications

et al. 2016

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Hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological vascular networks and engineer organs and tissues in vitro. The reason for this lies in the interesting features of this bioreactor type, including excellent mass transport properties. Indeed, hollow fiber bioreactors allow limitations to be overcome in nutrient transport by diffusion, which is often an obstacle to engineer sizable constructs in vitro. This work reviews the existing literature relevant to hollow fiber bioreactors in organ and tissue engineering applications. To this purpose, we first classify the hollow fiber bioreactors into 2 categories: cylindrical and rectangular. For each category, we summarize their main applications both at the tissue and at the organ level, focusing on experimental models and computational studies as predictive tools for designing innovative, dynamic culture systems. Finally, we discuss future perspectives on hollow fiber bioreactors as in vitro models for tissue and organ engineering applications.

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“…With the recent exponential growth of the field of tissue engineering [40,41], numerous scaffold materials and designs have been described in literature. Discussion of all different type of scaffold is beyond of scope of this thesis.…”

Section: Scaffoldsmentioning

confidence: 99%

“…Rapid prototyping is capable of directly producing complex, 3D scaffolds by joining liquids or by melt extrusion (bioplotter), powders (stereo-lithography), and sheet materials (fused by compression) one layer at a time using computer-aided design [36,41,42]. Rapid prototyping offers the potential to precisely control the morphology, geometry, and overall shape of the scaffold and may enable the creation of 3D scaffolds that matches the anatomical defects.…”

Section: Scaffoldsmentioning

confidence: 99%

“…cell attachment), whereas macro-pores (i.e. >50µm) influence tissue function, for example, pores 50-400µm in size are typically suggested for bone in-growth in relation to vascularity [39].With the recent exponential growth of the field of tissue engineering [40,41], numerous scaffold materials and designs have been described in literature. Discussion of all different type of scaffold is beyond of scope of this thesis.…”

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confidence: 99%

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Membrane supported scaffold architectures for tissue engineering

Bettahalli

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Prof. Dr. J. Vienken (Fresenius Medical Care, Germany) Dr. C. Legallais (Université de Technologie, France) Author -Narasimha Murthy Srivatsa BettahalliTitle -"Membrane supported scaffold architectures for tissue engineering"PhD Thesis, University of Twente, Enschede, The NetherlandsThe research described in this thesis was financially supported by STW (Utrecht, NL) (Project number -TKG.6716)Printed by: Gildeprint Drukkerijen, Enschede, The Netherlands Cover designed by NM Srivatsa BettahalliThe front cover shows dual flow glass bioreactor with 3D Free form fabricated scaffold integrated with hollow fibers (set-up artwork by Jonathan B Bennink of Tingle Visuals) and the back cover shows fluorescence microscope image of pre-labeled C2C12 cells cultured dynamically on multilayer cell-electrospun construct in dual flow perfusion bioreactor for 7 days (fluorescent image taken by Hemant Unadkat) and several SEM pictures at the top (PLLA hollow fiber, non-woven electrospun sheet and corrugated fiber) taken by the author. -3]. Along with the potential economic benefits from advanced tissue engineering technologies, reduced costs due to the availability of less expensive treatments for major medical problems is obvious, but indirect savings and dramatic improvements in treatment outcomes and quality of life for patients may prove to be even more important. There are several artificial tissues that are already being used for medical treatment (with limitations) which include fabricated skin, cartilage, blood vessels, bone, ligament and tendon [4][5][6]. It is also hoped that at least some of the whole organs like, liver, lung, kidney, pancreas, breast, intestine, etc. will become available off-the-shelf for treatment in the near future [7, 8]. MEMBRANE SUPPORTED SCAFFOLD ARCHITECTURES FOR TISSUE ENGINEERINGBesides the therapeutic application where the tissue is either grown in a patient (in-vivo) or outside the patient (in-vitro) and then transplanted to the patient, tissue engineering can have diagnostic applications where the tissue is made in-vitro and used for testing drug metabolism and uptake, toxicity, and pathogenicity [9][10][11][12]. The foundation of tissue engineering / regenerative medicine for either therapeutic or diagnostic applications is the ability to exploit living cells in a variety of ways.A tissue engineer first has to consider the function of the tissue -must it be strong? Elastic?Should it release certain proteins, like insulin from the pancreas, or filter toxins, like the kidneys? Then a 3D support must be designed that, when combined with cells, will eventually duplicate those functions, and continue to function properly in the body for years to come.Depending on the type of tissue, the design process can involve a variety of disciplines including mechanical / chemical engineering, molecular biology, physiology, medicine, polymer chemistry, and nanotechnology. Additionally, the scaffold can be constructed with or designed to release growth factors that can beneficially manipulate cell b...

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Modelling nutrient transport in hollow fibre membrane bioreactors for growing three-dimensional bone tissue

Cited by 74 publications

References 24 publications

The impact of inertial effects on solute dispersion in a channel with periodically varying aperture

The impact of inertial effects on solute dispersion in a channel with periodically varying aperture

Hollow Fiber Bioreactor Technology for Tissue Engineering Applications

Membrane supported scaffold architectures for tissue engineering

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