Modelling Learning and Memory in Drosophila to Understand Intellectual Disabilities

Mariano, Vittoria; Achsel, Tilmann; Bagni, Claudia; Kanellopoulos, Alexandros K.

doi:10.1016/j.neuroscience.2020.07.034

Cited by 32 publications

(25 citation statements)

References 280 publications

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“…Cognition ( Figure 1 F): For the untrained eye, Drosophila melanogaster seems to be a simple, unsophisticated organism, but the flies exhibit some advanced behaviors, such as courtship, learning and memory. Pavlovian conditioning is a test in which the flies are exposed to two odors, one of which is selectively avoided or selected due to previous experience of that odor with a negative (e.g., electric shocks) or positive (e.g., food with sugar) event [ 32 , 33 ]. In an additional test, courtship conditioning, the male courtship is suppressed as a result of the fly being continuously rejected by newly mated females [ 32 ].…”

Section: Methods To Study Proteotoxicity In Drosophilamentioning

confidence: 99%

“…Pavlovian conditioning is a test in which the flies are exposed to two odors, one of which is selectively avoided or selected due to previous experience of that odor with a negative (e.g., electric shocks) or positive (e.g., food with sugar) event [ 32 , 33 ]. In an additional test, courtship conditioning, the male courtship is suppressed as a result of the fly being continuously rejected by newly mated females [ 32 ]. For a short time after mating, females have no interest in mating again and are therefore unreceptive towards interested males.…”

Section: Methods To Study Proteotoxicity In Drosophilamentioning

confidence: 99%

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Exploring Aβ Proteotoxicity and Therapeutic Candidates Using Drosophila melanogaster

Elovsson

Bergkvist

Brorsson

2021

IJMS

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Alzheimer’s disease is a widespread and devastating neurological disorder associated with proteotoxic events caused by the misfolding and aggregation of the amyloid-β peptide. To find therapeutic strategies to combat this disease, Drosophila melanogaster has proved to be an excellent model organism that is able to uncover anti-proteotoxic candidates due to its outstanding genetic toolbox and resemblance to human disease genes. In this review, we highlight the use of Drosophila melanogaster to both study the proteotoxicity of the amyloid-β peptide and to screen for drug candidates. Expanding the knowledge of how the etiology of Alzheimer’s disease is related to proteotoxicity and how drugs can be used to block disease progression will hopefully shed further light on the field in the search for disease-modifying treatments.

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Section: Methods To Study Proteotoxicity In Drosophilamentioning

confidence: 99%

Section: Methods To Study Proteotoxicity In Drosophilamentioning

confidence: 99%

Exploring Aβ Proteotoxicity and Therapeutic Candidates Using Drosophila melanogaster

Elovsson

Bergkvist

Brorsson

2021

IJMS

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“…The small stem cell pool and mammal-like lineage composition make the Drosophila type II neural lineage an attractive model to study the complex brain development process. In addition, many important genes and signaling pathways are conserved throughout evolution (Homem and Knoblich, 2012;Mariano et al, 2020;Ogawa and Vallender, 2014), which makes revealing the molecular mechanisms of Drosophila type II neuron differentiation a meaningful primer to study the human analogs in the outer subventricular zone.…”

Section: Drosophila Type II Neural Lineages As a Model System To Study Complex Neurogenesis Processesmentioning

confidence: 99%

The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis

Michki

Sanjasaz

et al. 2021

Cell Reports

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The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third instar larval brains to study this process of the type II NB lineage. Combining prior knowledge, in silico analyses, and in situ validation, our multi-informatic investigation describes the molecular landscape from a single developmental snapshot. 17 markers are identified to differentiate distinct maturation stages. 30 markers are identified to specify the stem cell origin and/or cell division numbers of INPs, and at least 12 neuronal subtypes are identified. To foster future discoveries, we provide annotated tables of pairwise gene-gene correlation in single cells and MiCV, a web tool for interactively analyzing scRNA-seq datasets. Taken together, these resources advance our understanding of the neural differentiation process at the molecular level.

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“…Development from embryo to adult takes about 10 days and adult lifespan 1–2 months ( Johnson and Stolzing, 2019 ), allowing metabolic investigations on hundreds of offspring either at juvenile (larva and pupa) or adult stages. Given its powerful genetics, the Drosophila model has proved an efficient strategy for the study of several types of human pathologies, including metabolic ( Perrimon et al, 2016 ; Musselman and Kuhnlein, 2018 ; Baenas and Wagner, 2019 ), neurological ( Coll-Tane et al, 2019 ; Link and Bellen, 2020 ; Mariano et al, 2020 ; Salazar et al, 2020 ), cardiac ( Birse et al, 2010 ; Piazza and Wessells, 2011 ; Diop et al, 2015 ; Guida et al, 2019 ), digestive ( Musselman and Kuhnlein, 2018 ; Nayak and Mishra, 2019 ), and nephrocytic ( Millet-Boureima et al, 2018 ; Rani and Gautam, 2018 ) comorbidities. In addition, tumor models may be induced in larvae or adult flies by genome manipulation ( Pagliarini and Xu, 2003 ; Igaki et al, 2006 ; Dong et al, 2007 ; Gonzalez, 2013 ; Hirabayashi et al, 2013 ; Samji et al, 2021 ).…”

Section: The Fruit-fly Modelmentioning

confidence: 99%

Drosophila melanogaster: A Powerful Tiny Animal Model for the Study of Metabolic Hepatic Diseases

Moraes

Montagne

2021

Front. Physiol.

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Animal experimentation is limited by unethical procedures, time-consuming protocols, and high cost. Thus, the development of innovative approaches for disease treatment based on alternative models in a fast, safe, and economic manner is an important, yet challenging goal. In this paradigm, the fruit-fly Drosophila melanogaster has become a powerful model for biomedical research, considering its short life cycle and low-cost maintenance. In addition, biological processes are conserved and homologs of ∼75% of human disease-related genes are found in the fruit-fly. Therefore, this model has been used in innovative approaches to evaluate and validate the functional activities of candidate molecules identified via in vitro large-scale analyses, as putative agents to treat or reverse pathological conditions. In this context, Drosophila offers a powerful alternative to investigate the molecular aspects of liver diseases, since no effective therapies are available for those pathologies. Non-alcoholic fatty liver disease is the most common form of chronic hepatic dysfunctions, which may progress to the development of chronic hepatitis and ultimately to cirrhosis, thereby increasing the risk for hepatocellular carcinoma (HCC). This deleterious situation reinforces the use of the Drosophila model to accelerate functional research aimed at deciphering the mechanisms that sustain the disease. In this short review, we illustrate the relevance of using the fruit-fly to address aspects of liver pathologies to contribute to the biomedical area.

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Modelling Learning and Memory in Drosophila to Understand Intellectual Disabilities

Cited by 32 publications

References 280 publications

Exploring Aβ Proteotoxicity and Therapeutic Candidates Using Drosophila melanogaster

Exploring Aβ Proteotoxicity and Therapeutic Candidates Using Drosophila melanogaster

The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis

Drosophila melanogaster: A Powerful Tiny Animal Model for the Study of Metabolic Hepatic Diseases

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