Yasinovatka is one of around 30 prehistoric cemetery sites of fisher-hunter-foragers located along the Dnieper River in southern Ukraine. Dating to c. 5540-4930 cal BC, the skeletal remains at Yasinovatka suggest that around sixty-eight individuals were interred at the cemetery, during three broad phases of interment: A-type burials (c. 5540-4930 cal BC), Ƃ1 pit burials (c. 5550-4750 cal BC), and Ƃ2 pit burials (c. 4980-4460 cal BC). The burials are characterized, in part, by the inclusion of a number of Mariupol-type plates of boar tusk, in addition to deer tooth pendants, Unio shells, knife-like flint blades, Cyprinidae teeth, sherds of Neolithic pottery, and significant deposits of ochre in the later burial pits. Here we analyse δ 13 C and δ 15 N values for 50 human bone collagen samples from the site. The majority of the isotope results show a fisher-hunter-forager population reliant predominantly on freshwater aquatic proteins, which is in keeping with previous dietary isotope studies in the area. Two individuals however have δ 15 N values that are clearly depleted when compared with the main population; these reflect dietary protein intakes based on plant and animal terrestrial resources rather than the predominant focus on aquatic resources. Notably, the δ 13 C values of these anomalous individuals are not enriched compared with the fauna samples analysed from the region; this supports the possibility that they were incomers to the area, potentially from a nearby agrarian population.