Modelling hypercholesterolaemia in rats using high cholesterol diet

Cunha, Luiza Ferracini; Ongaratto, Mariana Aubin; Endres, Marcelo; Barschak, Alethéa Gatto

doi:10.1111/iep.12387

Cited by 16 publications

(10 citation statements)

References 46 publications

(75 reference statements)

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“…Decreased GSH coupled with increased MDA concentrations in heart and liver indicate hyperlipidemia and compromised antioxidant status. The elevations in serum cholesterol, TG and LDL levels observed in this study were in agreement with those reported in several studies (Binmowyna Alfaris, Almnaizel, Alsayadi, & Abdo al sanea, 2021;Cunha, Ongaratto, Endres, & Barschak, 2021). It has been reported that high serum abnormally levels of TG and LDL are associated with an increased risk for atherosclerosis (Nwozo, Kasumu, & Oyinloye, 2015).…”

Section: Discussionsupporting

confidence: 92%

The beneficial effects of virgin olive oil against oxidative stress induced by hypercholesterolemia in rats

Kribeche

Idoui

2022

Acta Sci. Health Sci.

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Cardiovascular disease (CVD) remains the major cause of mortality in the world, typically claiming a third of all deaths. The primary cause of CVD is atherosclerosis. Therefore, timely prevention and therapy of atherosclerosis are able to reduce the risk of the development of its clinical manifestations. Anti-atherosclerotic activity of medicinal plants mainly appears in their multiple effects. This study was carried out to evaluate the hypolipidemic activity of virgin olive oil in experimentally induced hyperlipemic Wistar. A total of 24 rats were randomly allocated to 4 equal groups and treated as follows for 50 days: (1) Normal control (NC); that were fed with a standart diet; (2) High Cholesterol Diet Control (HCD); which received high cholesterol diet for 50 days; (3) Animals receiving high cholesterol diet for 50 days, after this period the animals are fed for eight days by the standard food and receiving by gavage virgin olive oil (HCD+VOO) and (4) Animals fed for eight days with the standard food and receiving by gavage olive oil (VOO). High Cholesterol Diet containing yolk egg and coconut oil. Results showed that olive oil caused a significant (p < 0.01) reduction in serum levels of Total Cholesterol (TC), Triglycerides (TG), Low–Density Lipoprotein Cholesterol (LDL) and Atherogenic Index Serum (AIS). The results also demonstrated a significant (p < 0.01) increase in High–Density Lipoprotein Cholesterol (HDL). Moreover, virgin olive oil induced a significant reduction in liver lipid content. On the other hand, a High cholesterol diet induced oxidative stress was measured by estimating reduced glutathione level and amount of thiobarbituric acid reactive substances (TBARS) formed as an index of lipid peroxidation in a liver and a heart. Virgin olive oil supplementation attenuated all these variations. Our observations of the study indicate that the virgin olive oil has a significant antihyperlipidemic potential.

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Section: Discussionsupporting

confidence: 92%

The beneficial effects of virgin olive oil against oxidative stress induced by hypercholesterolemia in rats

Kribeche

Idoui

2022

Acta Sci. Health Sci.

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“…Hypercholesterolemia in animal models mimicked dyslipidemia in humans. Wistar rats induced by a high cholesterol feed can become a model of hypercholesterolemia (Cunha et al, 2021). Cholesterol levels of hypercholesterolemic rats in this study were more than 97 mg/dL.…”

Section: Discussionmentioning

confidence: 50%

Red dragon (Hylocereus polyrhizus) fruit peel extract increased the motility and viability of spermatozoa of hypercholesterolemic rats (Rattus norvegicus)

Niken Meyliana Sari,

M. Gandul Atik Yuliani,

Eduardus Bimo Aksono Herupradoto

et al. 2023

Ovz

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This study aims to determine the effect of red dragon (Hylocereus polyrhizus) fruit peel extract (RDFPE) on spermatozoa motility and viability of hypercholesterolemic rats (Rattus norvegicus) as a model. Twenty male rats were randomly divided into negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), and treatment 3 (T3) groups. All rats were given 2 mL of high cholesterol feed orally every day for 28 days. On day-15, all rats were measured for their blood cholesterol levels, followed by treatment for 14 days. Rats in the NC, PC, T1, T2, and T3 groups were treated with 1% Na-CMC, Simvastatin 10 mg/kg BW, and RDFPE of 500, 750, and 1000 mg/kg BW, respectively. On day-29, all rats were sacrificed to evaluate spermatozoa viability and motility. The results showed that spermatozoa viability and motility in the hypercholesterolemic rats (NC) group were the lowest (p <0.05) among the groups. Treatment of hypercholesterolemic rats with Simvastatin 10 mg/kg BW (PC) group showed higher (p <0.05) spermatozoa viability and motility compared to the NC group. RDFPE dose of 1000 mg/kg BW (T3 group) resulted in higher (p <0.05) spermatozoa viability and motility compared to other RDFPE doses (T1 and T2) and the control (NC) groups, and it was similar (p >0.05) compared with the Simvastatin treated (PC) group. It could be concluded that the administration of 1000 mg/kg BW ethanolic extract of red dragon fruit (Hylocereus polyrhizus) peel increased the viability and motility of spermatozoa of hypercholesterolemic rats (Rattus norvegicus) which were the same as the group of rats given Simvastatin 10 mg/kg BW.

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“…Because dietary intake of cholesterol is a critical factor in the etiopathogenesis of hypercholesterolemia, it is common for the HCD to induce hypercholesterolemia in rats ( Cunha et al, 2021 ). It was shown that rats fed a HCD for 4 weeks could be used as an experimental animal model for hypercholesterolemia, and these rats showed a significant increase in blood cholesterol, especially LDL ( Fidèle et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Paeoniflorin alleviates liver injury in hypercholesterolemic rats through the ROCK/AMPK pathway

et al. 2022

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Paeoniflorin (PF) is the main active component in Paeonia lactiflora Pall, and it has multiple effects. However, the precise mechanism of PF in hypercholesterolemia is unclear. In this study, rats were either fed a high-cholesterol diet (HCD) for 4 weeks to establish the hypercholesterolemic model or administered normal saline or PF (20 mg/kg/day). PF significantly reduced liver weight and the liver index. PF reduced hepatic lipid deposition and inflammation, improved serum lipid metabolism, and significantly inhibited serum and hepatic oxidative stress and the inflammatory response. PF treatment caused a marked decrease in the phosphorylated myosin phosphatase target subunit (p-MYPT)-1, nuclear sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthase (FAS) levels, and an increase in the low-density lipoprotein receptor (LDLR) and phosphorylated-AMP-activated protein kinase (p-AMPK). Thus, PF could alleviate liver injury in hypercholesterolemic rats, and the specific mechanism may be related to the antioxidant, anti-inflammatory properties, and ROCK/AMPK/SREBP-1c signaling pathway.

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Modelling hypercholesterolaemia in rats using high cholesterol diet

Abstract: The present review summarizes the current understanding of the metabolic profile involved in hypercholesterolaemia in rats.

Cited by 16 publications

References 46 publications

The beneficial effects of virgin olive oil against oxidative stress induced by hypercholesterolemia in rats

The beneficial effects of virgin olive oil against oxidative stress induced by hypercholesterolemia in rats

Red dragon (Hylocereus polyrhizus) fruit peel extract increased the motility and viability of spermatozoa of hypercholesterolemic rats (Rattus norvegicus)

Paeoniflorin alleviates liver injury in hypercholesterolemic rats through the ROCK/AMPK pathway

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